Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002221                                                              19     665
NP_001895                                                              19     665
NP_001091680                                                           19     665
NP_068831                                                              19     665
NP_001091679                                                           19     665
XP_006721941                                                           19     665
XP_006721940                                                           19     665
XP_006721939                                                           19     665
XP_006721938                                                           19     665
XP_006721937                                                           19     665
XP_006721936                                                           19     665
XP_006721935                                                           19     665
XP_006721934                                                           19     665
XP_005257370                                                           19     665
XP_006713048                                                           19     665
XP_006713047                                                           19     665
XP_006713046                                                           19     665
XP_005264943                                                           19     665
NP_079408                                                              17    2528
XP_006714089                                                           17    2528
XP_006714088                                                           17    2528
XP_005262715                                                           17    2528
NP_857594                                                              16    5631
XP_005270529                                                           16    5631
NP_001093207                                                           15    9605
NP_115708                                                              15    9605
>NP_002221
          Length = 745

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 258 EGAKMAVR 265
>NP_001895
          Length = 781

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 267 EGAKMAVR 274
>NP_001091680
          Length = 781

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 267 EGAKMAVR 274
>NP_068831
          Length = 745

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 258 EGAKMAVR 265
>NP_001091679
          Length = 781

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 267 EGAKMAVR 274
>XP_006721941
          Length = 745

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 258 EGAKMAVR 265
>XP_006721940
          Length = 745

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 258 EGAKMAVR 265
>XP_006721939
          Length = 745

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 258 EGAKMAVR 265
>XP_006721938
          Length = 745

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 258 EGAKMAVR 265
>XP_006721937
          Length = 745

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 258 EGAKMAVR 265
>XP_006721936
          Length = 745

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 258 EGAKMAVR 265
>XP_006721935
          Length = 762

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 275 EGAKMAVR 282
>XP_006721934
          Length = 783

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 296 EGAKMAVR 303
>XP_005257370
          Length = 783

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 296 EGAKMAVR 303
>XP_006713048
          Length = 720

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 267 EGAKMAVR 274
>XP_006713047
          Length = 774

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 260 EGAKMAVR 267
>XP_006713046
          Length = 774

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 260 EGAKMAVR 267
>XP_005264943
          Length = 781

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EGAKMAVR 8
           EGAKMAVR
Sbjct: 267 EGAKMAVR 274
>NP_079408
          Length = 1342

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EGAKMAVRF 9
           +GAKM  RF
Sbjct: 952 DGAKMVARF 960
>XP_006714089
          Length = 1281

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EGAKMAVRF 9
           +GAKM  RF
Sbjct: 952 DGAKMVARF 960
>XP_006714088
          Length = 1283

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EGAKMAVRF 9
           +GAKM  RF
Sbjct: 893 DGAKMVARF 901
>XP_005262715
          Length = 1320

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EGAKMAVRF 9
           +GAKM  RF
Sbjct: 930 DGAKMVARF 938
>NP_857594
          Length = 191

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  GAKMAVRF 9
          GA+ AVRF
Sbjct: 74 GARTAVRF 81
>XP_005270529
          Length = 241

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   GAKMAVRF 9
           GA+ AVRF
Sbjct: 124 GARTAVRF 131
>NP_001093207
          Length = 315

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  EGAKMAVRF 9
          +GA  AVRF
Sbjct: 25 QGAVRAVRF 33
>NP_115708
          Length = 315

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  EGAKMAVRF 9
          +GA  AVRF
Sbjct: 25 QGAVRAVRF 33
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.135    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 294,825
Number of extensions: 277
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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