Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     509
XP_005262424                                                           20     509
XP_005247327                                                           17    3301
XP_005247324                                                           17    3301
XP_006713624                                                           17    3301
XP_005247323                                                           17    3301
XP_005247322                                                           17    3301
NP_001159588                                                           17    4312
NP_003266                                                              17    4312
XP_005267581                                                           17    4312
NP_057212                                                              17    4312
NP_542377                                                              16    5631
NP_542378                                                              16    5631
NP_005983                                                              16    5631
NP_036292                                                              16    5631
NP_001265645                                                           16    5631
NP_003254                                                              16    5631
NP_004599                                                              16    5631
NP_005986                                                              16    5631
XP_006724375                                                           16    5631
XP_005261326                                                           16    5631
XP_005255580                                                           16    5631
XP_005266987                                                           16    5631
XP_006715521                                                           16    5631
XP_005262723                                                           16    5631
XP_005262722                                                           16    5631
XP_006714091                                                           16    5631
XP_005262720                                                           16    5631
XP_005262719                                                           16    5631
NP_001275949                                                           16    7355
NP_789786                                                              16    7355
NP_659464                                                              16    7355
XP_005276824                                                           16    7355
XP_005276823                                                           16    7355
XP_005276822                                                           16    7355
XP_006726278                                                           16    7355
XP_005276821                                                           16    7355
XP_006726277                                                           16    7355
XP_005276820                                                           16    7355
XP_005276819                                                           16    7355
XP_005276818                                                           16    7355
XP_006721643                                                           16    7355
XP_005248961                                                           16    7355
XP_005248520                                                           16    7355
XP_005248519                                                           16    7355
XP_005248518                                                           16    7355
XP_006714633                                                           16    7355
XP_005248517                                                           16    7355
XP_006714632                                                           16    7355
XP_005248516                                                           16    7355
XP_005248515                                                           16    7355
XP_005248514                                                           16    7355
NP_660152                                                              15    9605
>NP_722576
          Length = 3080

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EFSVLGTRM 9
            EF VLGTRM
Sbjct: 1862 EFPVLGTRM 1870
>XP_005262424
          Length = 2875

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EFSVLGTRM 9
            EF VLGTRM
Sbjct: 1657 EFPVLGTRM 1665
>XP_005247327
          Length = 274

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EFSVLGTR 8
           +FS LGTR
Sbjct: 257 QFSTLGTR 264
>XP_005247324
          Length = 429

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EFSVLGTR 8
           +FS LGTR
Sbjct: 412 QFSTLGTR 419
>XP_006713624
          Length = 432

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EFSVLGTR 8
           +FS LGTR
Sbjct: 415 QFSTLGTR 422
>XP_005247323
          Length = 435

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EFSVLGTR 8
           +FS LGTR
Sbjct: 418 QFSTLGTR 425
>XP_005247322
          Length = 435

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EFSVLGTR 8
           +FS LGTR
Sbjct: 418 QFSTLGTR 425
>NP_001159588
          Length = 359

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   EFSVLGTR 8
           +FS++GTR
Sbjct: 228 KFSIVGTR 235
>NP_003266
          Length = 359

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   EFSVLGTR 8
           +FS++GTR
Sbjct: 228 KFSIVGTR 235
>XP_005267581
          Length = 509

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  FSVLGTRM 9
          FS+LGT M
Sbjct: 7  FSLLGTEM 14
>NP_057212
          Length = 874

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EFSVLGTRM 9
           EF+VL TR+
Sbjct: 447 EFTVLATRI 455
>NP_542377
          Length = 398

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EFSVLGTRM 9
           EF+ LGT M
Sbjct: 122 EFNQLGTEM 130
>NP_542378
          Length = 495

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EFSVLGTRM 9
           EF+ LGT M
Sbjct: 122 EFNQLGTEM 130
>NP_005983
          Length = 372

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EFSVLGTRM 9
           EF+ LGT M
Sbjct: 122 EFNQLGTEM 130
>NP_036292
          Length = 621

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EFSVLGTRM 9
           +  +LGTRM
Sbjct: 563 QLDILGTRM 571
>NP_001265645
          Length = 621

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EFSVLGTRM 9
           +  +LGTRM
Sbjct: 563 QLDILGTRM 571
>NP_003254
          Length = 786

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FSVLGTRM 9
           F+V GTRM
Sbjct: 331 FTVSGTRM 338
>NP_004599
          Length = 436

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EFSVLGTRM 9
           EFS +GT M
Sbjct: 103 EFSSVGTEM 111
>NP_005986
          Length = 385

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  EFSVLGTRM 9
          EF+ LGT M
Sbjct: 77 EFNQLGTEM 85
>XP_006724375
          Length = 495

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EFSVLGTRM 9
           EF+ LGT M
Sbjct: 122 EFNQLGTEM 130
>XP_005261326
          Length = 545

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EFSVLGTRM 9
           EF+ LGT M
Sbjct: 172 EFNQLGTEM 180
>XP_005255580
          Length = 436

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EFSVLGTRM 9
           EFS +GT M
Sbjct: 103 EFSSVGTEM 111
>XP_005266987
          Length = 597

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EFSVLGTRM 9
           +  +LGTRM
Sbjct: 539 QLDILGTRM 547
>XP_006715521
          Length = 621

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EFSVLGTRM 9
           +  +LGTRM
Sbjct: 563 QLDILGTRM 571
>XP_005262723
          Length = 786

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FSVLGTRM 9
           F+V GTRM
Sbjct: 331 FTVSGTRM 338
>XP_005262722
          Length = 786

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FSVLGTRM 9
           F+V GTRM
Sbjct: 331 FTVSGTRM 338
>XP_006714091
          Length = 786

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FSVLGTRM 9
           F+V GTRM
Sbjct: 331 FTVSGTRM 338
>XP_005262720
          Length = 786

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FSVLGTRM 9
           F+V GTRM
Sbjct: 331 FTVSGTRM 338
>XP_005262719
          Length = 786

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FSVLGTRM 9
           F+V GTRM
Sbjct: 331 FTVSGTRM 338
>NP_001275949
          Length = 797

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EFSVLGTR 8
           +FS+LG R
Sbjct: 533 DFSILGKR 540
>NP_789786
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 463 EFSVLG 468
>NP_659464
          Length = 814

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EFSVLGTR 8
           +FS+LG R
Sbjct: 533 DFSILGKR 540
>XP_005276824
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 338 EFSVLG 343
>XP_005276823
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 338 EFSVLG 343
>XP_005276822
          Length = 421

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 373 EFSVLG 378
>XP_006726278
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 386 EFSVLG 391
>XP_005276821
          Length = 438

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 390 EFSVLG 395
>XP_006726277
          Length = 506

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 458 EFSVLG 463
>XP_005276820
          Length = 510

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 462 EFSVLG 467
>XP_005276819
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 463 EFSVLG 468
>XP_005276818
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 463 EFSVLG 468
>XP_006721643
          Length = 345

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EFSVLGTRM 9
           E SVLG+R+
Sbjct: 328 EASVLGSRL 336
>XP_005248961
          Length = 842

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EFSVLGTR 8
           +FS+LG R
Sbjct: 533 DFSILGKR 540
>XP_005248520
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 338 EFSVLG 343
>XP_005248519
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 338 EFSVLG 343
>XP_005248518
          Length = 421

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 373 EFSVLG 378
>XP_006714633
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 386 EFSVLG 391
>XP_005248517
          Length = 438

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 390 EFSVLG 395
>XP_006714632
          Length = 506

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 458 EFSVLG 463
>XP_005248516
          Length = 510

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 462 EFSVLG 467
>XP_005248515
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 463 EFSVLG 468
>XP_005248514
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EFSVLG 6
           EFSVLG
Sbjct: 463 EFSVLG 468
>NP_660152
          Length = 159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  FSVLGT 7
          FS+LGT
Sbjct: 47 FSILGT 52
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.139    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 430,426
Number of extensions: 645
Number of successful extensions: 53
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 53
Number of HSP's successfully gapped: 53
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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