Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              18    1135
NP_001035957                                                           18    1135
XP_006721991                                                           18    1135
XP_006721990                                                           18    1135
XP_006721989                                                           18    1135
XP_005258041                                                           18    1135
XP_006721988                                                           18    1135
XP_006721987                                                           18    1135
XP_006721986                                                           18    1135
XP_005258040                                                           18    1135
XP_006721985                                                           18    1135
NP_006002                                                              17    3301
NP_001004441                                                           16    7355
XP_006714662                                                           16    7355
NP_001275514                                                           15    9605
NP_741982                                                              15    9605
NP_203744                                                              15    9605
NP_116246                                                              15    9605
XP_006724408                                                           15    9605
XP_005261849                                                           15    9605
XP_005261848                                                           15    9605
XP_005254200                                                           15    9605
NP_001005326                                                           15    9605
>NP_000258
          Length = 2818

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EFKALKMLS 9
            EFKALK LS
Sbjct: 1562 EFKALKTLS 1570
>NP_001035957
          Length = 2839

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EFKALKMLS 9
            EFKALK LS
Sbjct: 1583 EFKALKTLS 1591
>XP_006721991
          Length = 1632

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EFKALKMLS 9
            EFKALK LS
Sbjct: 1593 EFKALKTLS 1601
>XP_006721990
          Length = 2823

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EFKALKMLS 9
            EFKALK LS
Sbjct: 1593 EFKALKTLS 1601
>XP_006721989
          Length = 2823

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EFKALKMLS 9
            EFKALK LS
Sbjct: 1593 EFKALKTLS 1601
>XP_005258041
          Length = 2836

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EFKALKMLS 9
            EFKALK LS
Sbjct: 1562 EFKALKTLS 1570
>XP_006721988
          Length = 2846

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EFKALKMLS 9
            EFKALK LS
Sbjct: 1572 EFKALKTLS 1580
>XP_006721987
          Length = 2849

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EFKALKMLS 9
            EFKALK LS
Sbjct: 1593 EFKALKTLS 1601
>XP_006721986
          Length = 2854

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EFKALKMLS 9
            EFKALK LS
Sbjct: 1580 EFKALKTLS 1588
>XP_005258040
          Length = 2857

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EFKALKMLS 9
            EFKALK LS
Sbjct: 1583 EFKALKTLS 1591
>XP_006721985
          Length = 2867

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EFKALKMLS 9
            EFKALK LS
Sbjct: 1593 EFKALKTLS 1601
>NP_006002
          Length = 375

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   EFKALKML 8
           EFKALK L
Sbjct: 351 EFKALKSL 358
>NP_001004441
          Length = 514

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EFKALKML 8
           EFK+ KML
Sbjct: 490 EFKSKKML 497
>XP_006714662
          Length = 514

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EFKALKML 8
           EFK+ KML
Sbjct: 490 EFKSKKML 497
>NP_001275514
          Length = 332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FKALKML 8
           +KALKM+
Sbjct: 236 YKALKMM 242
>NP_741982
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FKALKML 8
           +KALKM+
Sbjct: 236 YKALKMM 242
>NP_203744
          Length = 863

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EFKALKML 8
           +FK +KML
Sbjct: 838 KFKTMKML 845
>NP_116246
          Length = 547

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FKALKML 8
           +KALKM+
Sbjct: 236 YKALKMM 242
>XP_006724408
          Length = 788

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EFKALKML 8
           +FK +KML
Sbjct: 763 KFKTMKML 770
>XP_005261849
          Length = 870

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EFKALKML 8
           +FK +KML
Sbjct: 845 KFKTMKML 852
>XP_005261848
          Length = 872

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EFKALKML 8
           +FK +KML
Sbjct: 847 KFKTMKML 854
>XP_005254200
          Length = 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   FKALKML 8
           +KALKM+
Sbjct: 236 YKALKMM 242
>NP_001005326
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   FKALKMLS 9
           FKA  MLS
Sbjct: 234 FKAFSMLS 241
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.131    0.318 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 269,303
Number of extensions: 300
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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