Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              20     509
NP_001256971                                                           17    2528
NP_001256970                                                           17    2528
NP_115748                                                              17    2528
NP_008919                                                              17    4312
NP_008969                                                              17    4312
NP_001191762                                                           16    5631
NP_001191760                                                           16    5631
NP_060545                                                              16    5631
NP_001191761                                                           16    5631
XP_005265322                                                           16    5631
NP_476506                                                              16    7355
NP_476505                                                              16    7355
NP_004360                                                              16    7355
NP_476508                                                              16    7355
NP_476507                                                              16    7355
XP_005246123                                                           16    7355
XP_005246122                                                           16    7355
XP_006712316                                                           16    7355
NP_003493                                                              15    9605
NP_851393                                                              15    9605
NP_009029                                                              15    9605
NP_658911                                                              15    9605
NP_005788                                                              15    9605
NP_001826                                                              15    9605
NP_001137505                                                           15    9605
NP_004090                                                              15    9605
NP_001257456                                                           15    9605
NP_001257455                                                           15    9605
XP_006724391                                                           15    9605
XP_005261338                                                           15    9605
XP_005277858                                                           15    9605
XP_006724390                                                           15    9605
XP_006724389                                                           15    9605
XP_005277857                                                           15    9605
XP_005255666                                                           15    9605
XP_005255665                                                           15    9605
XP_005255664                                                           15    9605
XP_005270453                                                           15    9605
XP_005270452                                                           15    9605
XP_005270451                                                           15    9605
NP_660329                                                              15    9605
NP_006836                                                              15    9605
>NP_000312
          Length = 928

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EEYDSSIVF 9
           EEYDS IVF
Sbjct: 747 EEYDSIIVF 755
>NP_001256971
          Length = 191

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  EEYDSSI 7
          EEYDSS+
Sbjct: 73 EEYDSSL 79
>NP_001256970
          Length = 177

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  EEYDSSI 7
          EEYDSS+
Sbjct: 59 EEYDSSL 65
>NP_115748
          Length = 177

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  EEYDSSI 7
          EEYDSS+
Sbjct: 59 EEYDSSL 65
>NP_008919
          Length = 967

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EEYDSSIVF 9
           E YD++I+F
Sbjct: 348 EHYDTAILF 356
>NP_008969
          Length = 930

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EEYDSSIVF 9
           E YD++I+F
Sbjct: 357 EHYDAAILF 365
>NP_001191762
          Length = 549

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   EEYDSSIVF 9
           E+YD  ++F
Sbjct: 127 EDYDKYVIF 135
>NP_001191760
          Length = 627

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   EEYDSSIVF 9
           E+YD  ++F
Sbjct: 238 EDYDKYVIF 246
>NP_060545
          Length = 660

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   EEYDSSIVF 9
           E+YD  ++F
Sbjct: 238 EDYDKYVIF 246
>NP_001191761
          Length = 594

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   EEYDSSIVF 9
           E+YD  ++F
Sbjct: 172 EDYDKYVIF 180
>XP_005265322
          Length = 621

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   EEYDSSIVF 9
           E+YD  ++F
Sbjct: 238 EDYDKYVIF 246
>NP_476506
          Length = 1237

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   YDSSIVF 9
           +DSS+VF
Sbjct: 391 FDSSLVF 397
>NP_476505
          Length = 1036

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   YDSSIVF 9
           +DSS+VF
Sbjct: 190 FDSSLVF 196
>NP_004360
          Length = 3177

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   YDSSIVF 9
           +DSS+VF
Sbjct: 597 FDSSLVF 603
>NP_476508
          Length = 2971

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   YDSSIVF 9
           +DSS+VF
Sbjct: 391 FDSSLVF 397
>NP_476507
          Length = 2570

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   YDSSIVF 9
           +DSS+VF
Sbjct: 190 FDSSLVF 196
>XP_005246123
          Length = 2770

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   YDSSIVF 9
           +DSS+VF
Sbjct: 190 FDSSLVF 196
>XP_005246122
          Length = 2977

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   YDSSIVF 9
           +DSS+VF
Sbjct: 597 FDSSLVF 603
>XP_006712316
          Length = 3010

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   YDSSIVF 9
           +DSS+VF
Sbjct: 597 FDSSLVF 603
>NP_003493
          Length = 862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   EEYDSSIVF 9
           +E+D  +VF
Sbjct: 829 DEFDCGVVF 837
>NP_851393
          Length = 826

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   EEYDSSIVF 9
           +E+D  +VF
Sbjct: 793 DEFDCGVVF 801
>NP_009029
          Length = 1640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YDSSIV 8
           YDSS+V
Sbjct: 900 YDSSVV 905
>NP_658911
          Length = 215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EEYDSSIV 8
           E YD+SI+
Sbjct: 102 ERYDASIL 109
>NP_005788
          Length = 215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EEYDSSIV 8
           E YD+SI+
Sbjct: 102 ERYDASIL 109
>NP_001826
          Length = 1583

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YDSSIV 8
           YDSS+V
Sbjct: 900 YDSSVV 905
>NP_001137505
          Length = 282

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  EEYDSSIVF 9
          +EY+ ++VF
Sbjct: 46 KEYERAVVF 54
>NP_004090
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  EEYDSSIVF 9
          +EY+ +I+F
Sbjct: 58 KEYERAIIF 66
>NP_001257456
          Length = 168

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  EEYDSSIVF 9
          +EY+ +I+F
Sbjct: 58 KEYERAIIF 66
>NP_001257455
          Length = 197

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  EEYDSSIVF 9
          +EY+ +I+F
Sbjct: 58 KEYERAIIF 66
>XP_006724391
          Length = 1567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YDSSIV 8
           YDSS+V
Sbjct: 921 YDSSVV 926
>XP_005261338
          Length = 1570

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YDSSIV 8
           YDSS+V
Sbjct: 921 YDSSVV 926
>XP_005277858
          Length = 1604

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YDSSIV 8
           YDSS+V
Sbjct: 921 YDSSVV 926
>XP_006724390
          Length = 1623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YDSSIV 8
           YDSS+V
Sbjct: 883 YDSSVV 888
>XP_006724389
          Length = 1653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YDSSIV 8
           YDSS+V
Sbjct: 921 YDSSVV 926
>XP_005277857
          Length = 1661

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   YDSSIV 8
           YDSS+V
Sbjct: 921 YDSSVV 926
>XP_005255666
          Length = 862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   EEYDSSIVF 9
           +E+D  +VF
Sbjct: 829 DEFDCGVVF 837
>XP_005255665
          Length = 875

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   EEYDSSIVF 9
           +E+D  +VF
Sbjct: 842 DEFDCGVVF 850
>XP_005255664
          Length = 911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   EEYDSSIVF 9
           +E+D  +VF
Sbjct: 878 DEFDCGVVF 886
>XP_005270453
          Length = 671

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EEYDSS 6
           +EYDSS
Sbjct: 167 QEYDSS 172
>XP_005270452
          Length = 684

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EEYDSS 6
           +EYDSS
Sbjct: 180 QEYDSS 185
>XP_005270451
          Length = 716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EEYDSS 6
           +EYDSS
Sbjct: 212 QEYDSS 217
>NP_660329
          Length = 291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  EEYDSSIVF 9
          +EY+ ++VF
Sbjct: 55 KEYERAVVF 63
>NP_006836
          Length = 725

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EEYDSS 6
           +EYDSS
Sbjct: 221 QEYDSS 226
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.132    0.344 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 503,188
Number of extensions: 712
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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