Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              18    1935
XP_005262424                                                           18    1935
NP_001093321                                                           17    2528
NP_001038945                                                           17    2528
NP_071900                                                              16    5631
NP_001230175                                                           16    5631
XP_006714967                                                           16    5631
XP_006714966                                                           16    5631
XP_006714964                                                           16    5631
XP_005266016                                                           16    5631
NP_001269316                                                           16    7355
NP_005887                                                              16    7355
NP_001269315                                                           16    7355
NP_001159756                                                           16    7355
NP_006420                                                              16    7355
NP_001009570                                                           16    7355
NP_001159757                                                           16    7355
NP_001271203                                                           16    7355
NP_877497                                                              16    7355
XP_005269612                                                           16    7355
NP_055093                                                              15    9605
NP_775871                                                              15    9605
XP_006714203                                                           15    9605
>NP_722576
          Length = 3080

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EETSTNALS 9
            EETST ALS
Sbjct: 1178 EETSTYALS 1186
>XP_005262424
          Length = 2875

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EETSTNALS 9
           EETST ALS
Sbjct: 973 EETSTYALS 981
>NP_001093321
          Length = 474

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   ETSTNAL 8
           ETSTNAL
Sbjct: 388 ETSTNAL 394
>NP_001038945
          Length = 474

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   ETSTNAL 8
           ETSTNAL
Sbjct: 388 ETSTNAL 394
>NP_071900
          Length = 2696

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EETSTNA 7
           EET TNA
Sbjct: 185 EETQTNA 191
>NP_001230175
          Length = 804

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ETSTNALS 9
           +TS+NALS
Sbjct: 728 DTSSNALS 735
>XP_006714967
          Length = 1327

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EETSTNA 7
           EET TNA
Sbjct: 185 EETQTNA 191
>XP_006714966
          Length = 1499

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EETSTNA 7
           EET TNA
Sbjct: 185 EETQTNA 191
>XP_006714964
          Length = 2696

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EETSTNA 7
           EET TNA
Sbjct: 185 EETQTNA 191
>XP_005266016
          Length = 2696

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   EETSTNA 7
           EET TNA
Sbjct: 185 EETQTNA 191
>NP_001269316
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EETSTNALS 9
           +ETSTN ++
Sbjct: 323 QETSTNPIA 331
>NP_005887
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EETSTNALS 9
           +ETSTN ++
Sbjct: 323 QETSTNPIA 331
>NP_001269315
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EETSTNALS 9
           +ETSTN ++
Sbjct: 323 QETSTNPIA 331
>NP_001159756
          Length = 456

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ETSTNALS 9
           +TS NALS
Sbjct: 244 QTSVNALS 251
>NP_006420
          Length = 543

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ETSTNALS 9
           +TS NALS
Sbjct: 331 QTSVNALS 338
>NP_001009570
          Length = 339

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ETSTNALS 9
           +TS NALS
Sbjct: 127 QTSVNALS 134
>NP_001159757
          Length = 499

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ETSTNALS 9
           +TS NALS
Sbjct: 287 QTSVNALS 294
>NP_001271203
          Length = 780

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ETSTNAL 8
           ETSTN L
Sbjct: 736 ETSTNCL 742
>NP_877497
          Length = 776

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ETSTNAL 8
           ETSTN L
Sbjct: 732 ETSTNCL 738
>XP_005269612
          Length = 669

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ETSTNAL 8
           ETSTN L
Sbjct: 625 ETSTNCL 631
>NP_055093
          Length = 839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EETSTNAL 8
           +ETS NAL
Sbjct: 126 KETSENAL 133
>NP_775871
          Length = 8384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    TSTNALS 9
            T TNALS
Sbjct: 5250 TGTNALS 5256
>XP_006714203
          Length = 543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EETSTNAL 8
           +ETS NAL
Sbjct: 126 KETSENAL 133
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.270   0.0899    0.192 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 344,610
Number of extensions: 376
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.4 bits)
S2: 28 (15.4 bits)

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