Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              18    1135
NP_005180                                                              17    3301
XP_006722203                                                           17    3301
NP_004517                                                              17    4312
NP_002760                                                              16    5631
NP_001130027                                                           16    7355
NP_001130026                                                           16    7355
NP_055238                                                              16    7355
NP_036422                                                              16    7355
XP_006714650                                                           16    7355
XP_005272020                                                           16    7355
XP_006712946                                                           16    7355
XP_005247038                                                           16    7355
NP_612490                                                              15    9605
XP_005261428                                                           15    9605
XP_005261427                                                           15    9605

>NP_004251
          Length = 1208

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EEKLSSRAV 9
            EEK SSRAV
Sbjct: 1152 EEKFSSRAV 1160



>NP_005180
          Length = 532

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EKLSSRAV 9
           EKL+SRAV
Sbjct: 412 EKLASRAV 419



>XP_006722203
          Length = 447

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EKLSSRAV 9
           EKL+SRAV
Sbjct: 327 EKLASRAV 334



>NP_004517
          Length = 904

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EKLSSRAV 9
           EK+SSRA+
Sbjct: 537 EKVSSRAI 544



>NP_002760
          Length = 247

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   KLSSRAV 9
           KLSSRAV
Sbjct: 112 KLSSRAV 118



>NP_001130027
          Length = 670

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EEKLSSR 7
           +EKLSSR
Sbjct: 188 QEKLSSR 194



>NP_001130026
          Length = 718

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EEKLSSR 7
           +EKLSSR
Sbjct: 236 QEKLSSR 242



>NP_055238
          Length = 1163

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  EEKLSSR 7
          E+KLSSR
Sbjct: 45 EDKLSSR 51



>NP_036422
          Length = 766

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EEKLSSR 7
           +EKLSSR
Sbjct: 284 QEKLSSR 290



>XP_006714650
          Length = 1134

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  EEKLSSR 7
          E+KLSSR
Sbjct: 45 EDKLSSR 51



>XP_005272020
          Length = 1163

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  EEKLSSR 7
          E+KLSSR
Sbjct: 45 EDKLSSR 51



>XP_006712946
          Length = 413

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EEKLSSR 7
           +EKLSSR
Sbjct: 284 QEKLSSR 290



>XP_005247038
          Length = 549

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  EEKLSSR 7
          +EKLSSR
Sbjct: 67 QEKLSSR 73



>NP_612490
          Length = 762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EEKLSSRAV 9
           EE+L  RAV
Sbjct: 365 EEELEERAV 373



>XP_005261428
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EEKLSSRAV 9
           EE+L  RAV
Sbjct: 365 EEELEERAV 373



>XP_005261427
          Length = 771

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EEKLSSRAV 9
           EE+L  RAV
Sbjct: 365 EEELEERAV 373



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.292    0.108    0.232 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 458,674
Number of extensions: 351
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)