Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001159579                                                           19     869
NP_005509                                                              19     869
NP_005742                                                              18    1135
NP_671714                                                              18    1135
XP_006715890                                                           18    1135
XP_005250160                                                           18    1135
XP_005250159                                                           18    1135
NP_003213                                                              18    1482
NP_001615                                                              18    1482
XP_005266896                                                           18    1482
NP_937870                                                              16    5631
NP_937872                                                              16    5631
NP_116098                                                              16    5631
XP_006718108                                                           16    5631
XP_006713970                                                           16    5631
XP_006713968                                                           16    5631
NP_002917                                                              16    5631
NP_000161                                                              16    7355
NP_001171536                                                           16    7355
NP_000379                                                              16    7355
NP_620482                                                              16    7355
NP_001077084                                                           16    7355
NP_001257903                                                           16    7355
NP_056492                                                              16    7355
NP_001153069                                                           16    7355
NP_060639                                                              16    7355
XP_006721269                                                           16    7355
XP_006719162                                                           16    7355
XP_005253462                                                           16    7355
XP_005247894                                                           16    7355
XP_006713853                                                           16    7355
XP_005247893                                                           16    7355
XP_006713852                                                           16    7355
XP_006712362                                                           16    7355
NP_000433                                                              15    9605
NP_659455                                                              15    9605
XP_005276940                                                           15    9605
XP_005276939                                                           15    9605
XP_005276938                                                           15    9605
XP_005276937                                                           15    9605
XP_006722008                                                           15    9605
XP_006722007                                                           15    9605
NP_057359                                                              15    9605
>NP_001159579
          Length = 466

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   EEFTEIISQ 9
           EEFTEI++Q
Sbjct: 416 EEFTEIMNQ 424
>NP_005509
          Length = 508

 Score = 18.9 bits (37), Expect =   869
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   EEFTEIISQ 9
           EEFTEI++Q
Sbjct: 458 EEFTEIMNQ 466
>NP_005742
          Length = 3907

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EEFTEIISQ 9
            EE TEIISQ
Sbjct: 2815 EEVTEIISQ 2823
>NP_671714
          Length = 3899

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EEFTEIISQ 9
            EE TEIISQ
Sbjct: 2807 EEVTEIISQ 2815
>XP_006715890
          Length = 3860

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EEFTEIISQ 9
            EE TEIISQ
Sbjct: 2768 EEVTEIISQ 2776
>XP_005250160
          Length = 3939

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EEFTEIISQ 9
            EE TEIISQ
Sbjct: 2847 EEVTEIISQ 2855
>XP_005250159
          Length = 3952

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EEFTEIISQ 9
            EE TEIISQ
Sbjct: 2860 EEVTEIISQ 2868
>NP_003213
          Length = 450

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EEFTEIISQ 9
           +EFTE++SQ
Sbjct: 360 KEFTELLSQ 368
>NP_001615
          Length = 1723

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EEFTEIISQ 9
           E+FTEII Q
Sbjct: 698 EKFTEIIKQ 706
>XP_005266896
          Length = 2131

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    EEFTEIISQ 9
            E+FTEII Q
Sbjct: 1106 EKFTEIIKQ 1114
>NP_937870
          Length = 799

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EEFTEIISQ 9
           EEF E+IS+
Sbjct: 540 EEFFELISK 548
>NP_937872
          Length = 1447

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EEFTEIISQ 9
            EEF E+IS+
Sbjct: 1188 EEFFELISK 1196
>NP_116098
          Length = 581

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EEFTEIISQ 9
           EEF E ISQ
Sbjct: 359 EEFLERISQ 367
>XP_006718108
          Length = 560

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EEFTEIISQ 9
           EEF E ISQ
Sbjct: 359 EEFLERISQ 367
>XP_006713970
          Length = 646

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EEFTEIISQ 9
           EEF E+IS+
Sbjct: 387 EEFFELISK 395
>XP_006713968
          Length = 1447

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EEFTEIISQ 9
            EEF E+IS+
Sbjct: 1188 EEFFELISK 1196
>NP_002917
          Length = 1376

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EEFTEIISQ 9
            EEF E+IS+
Sbjct: 1188 EEFFELISK 1196
>NP_000161
          Length = 1020

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   EEFTEIISQ 9
           E+FTE++ +
Sbjct: 274 EDFTELVER 282
>NP_001171536
          Length = 1088

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EFTEIISQ 9
           +F+E+ISQ
Sbjct: 238 DFSELISQ 245
>NP_000379
          Length = 1078

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EFTEIISQ 9
           +F+E+ISQ
Sbjct: 238 DFSELISQ 245
>NP_620482
          Length = 1341

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   EEFTEIISQ 9
           EEF   ISQ
Sbjct: 264 EEFNHFISQ 272
>NP_001077084
          Length = 1314

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   EEFTEIISQ 9
           EEF   ISQ
Sbjct: 264 EEFNHFISQ 272
>NP_001257903
          Length = 5121

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    EFTEII 7
            EFTEII
Sbjct: 3634 EFTEII 3639
>NP_056492
          Length = 342

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  EFTEIISQ 9
          EFTEI S+
Sbjct: 22 EFTEITSR 29
>NP_001153069
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  EFTEIISQ 9
          EFTEI S+
Sbjct: 22 EFTEITSR 29
>NP_060639
          Length = 1747

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EEFTEIISQ 9
            E  TE++SQ
Sbjct: 1138 EPITEVVSQ 1146
>XP_006721269
          Length = 5138

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    EFTEII 7
            EFTEII
Sbjct: 3651 EFTEII 3656
>XP_006719162
          Length = 1676

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EEFTEIISQ 9
            E  TE++SQ
Sbjct: 1138 EPITEVVSQ 1146
>XP_005253462
          Length = 1747

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EEFTEIISQ 9
            E  TE++SQ
Sbjct: 1138 EPITEVVSQ 1146
>XP_005247894
          Length = 917

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  EFTEIISQ 9
          +F+E+ISQ
Sbjct: 77 DFSELISQ 84
>XP_006713853
          Length = 1078

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EFTEIISQ 9
           +F+E+ISQ
Sbjct: 238 DFSELISQ 245
>XP_005247893
          Length = 1078

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EFTEIISQ 9
           +F+E+ISQ
Sbjct: 238 DFSELISQ 245
>XP_006713852
          Length = 1078

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EFTEIISQ 9
           +F+E+ISQ
Sbjct: 238 DFSELISQ 245
>XP_006712362
          Length = 1350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   EEFTEIISQ 9
           EEF   ISQ
Sbjct: 273 EEFNHFISQ 281
>NP_000433
          Length = 738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EEFTEIISQ 9
           +EF EII Q
Sbjct: 265 QEFPEIIIQ 273
>NP_659455
          Length = 998

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EFTEIISQ 9
           EFT I+SQ
Sbjct: 244 EFTCIVSQ 251
>XP_005276940
          Length = 706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EEFTEIISQ 9
           +EF EII Q
Sbjct: 265 QEFPEIIIQ 273
>XP_005276939
          Length = 717

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EEFTEIISQ 9
           +EF EII Q
Sbjct: 265 QEFPEIIIQ 273
>XP_005276938
          Length = 719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EEFTEIISQ 9
           +EF EII Q
Sbjct: 265 QEFPEIIIQ 273
>XP_005276937
          Length = 727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EEFTEIISQ 9
           +EF EII Q
Sbjct: 265 QEFPEIIIQ 273
>XP_006722008
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EEFTEIISQ 9
           +EF EII Q
Sbjct: 265 QEFPEIIIQ 273
>XP_006722007
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EEFTEIISQ 9
           +EF EII Q
Sbjct: 265 QEFPEIIIQ 273
>NP_057359
          Length = 195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  EEFTEIISQ 9
          EE+  +ISQ
Sbjct: 56 EEYMRVISQ 64
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.306    0.127    0.298 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 407,943
Number of extensions: 697
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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