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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              20     299
XP_006713721                                                           20     299
NP_060087                                                              17    3301
NP_079107                                                              17    4312
XP_006716549                                                           17    4312
XP_006716548                                                           17    4312
XP_005251385                                                           17    4312
XP_005245852                                                           16    5631
XP_005245853                                                           16    5631
XP_005251137                                                           15    9605
NP_004465                                                              15    9605

>NP_006209
          Length = 1068

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EECQIMSSA 9
           EEC+IMSSA
Sbjct: 767 EECRIMSSA 775



>XP_006713721
          Length = 1068

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EECQIMSSA 9
           EEC+IMSSA
Sbjct: 767 EECRIMSSA 775



>NP_060087
          Length = 2555

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EECQIMSSA 9
           +ECQ+M +A
Sbjct: 297 DECQLMPNA 305



>NP_079107
          Length = 853

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EECQIMSSA 9
           E+CQ +SSA
Sbjct: 571 EKCQSVSSA 579



>XP_006716549
          Length = 646

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EECQIMSSA 9
           E+CQ +SSA
Sbjct: 478 EKCQSVSSA 486



>XP_006716548
          Length = 739

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EECQIMSSA 9
           E+CQ +SSA
Sbjct: 571 EKCQSVSSA 579



>XP_005251385
          Length = 760

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EECQIMSSA 9
           E+CQ +SSA
Sbjct: 478 EKCQSVSSA 486



>XP_005245852
          Length = 873

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  EECQIMSSA 9
          ++CQ+M +A
Sbjct: 33 QQCQLMQAA 41



>XP_005245853
          Length = 467

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  EECQIMSSA 9
          ++CQ+M +A
Sbjct: 33 QQCQLMQAA 41



>XP_005251137
          Length = 157

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   EECQIMSSA 9
           E C + SSA
Sbjct: 139 ERCHLFSSA 147



>NP_004465
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  CQIMSS 8
          C+IMSS
Sbjct: 8  CEIMSS 13



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.111    0.294 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 599,525
Number of extensions: 272
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)