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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001167558                                                           18    1135
NP_858045                                                              18    1135
NP_006525                                                              18    1135
NP_001167559                                                           18    1135
NP_690850                                                              17    3301
NP_006556                                                              16    5631
NP_005302                                                              16    5631
NP_001001549                                                           16    5631
XP_005255832                                                           16    5631
XP_006715767                                                           16    5631
XP_006715766                                                           16    5631
XP_005271818                                                           16    5631
XP_005271817                                                           16    5631
NP_001171989                                                           16    7355
NP_001171991                                                           16    7355
NP_001171990                                                           16    7355
NP_932766                                                              16    7355
NP_055464                                                              16    7355
NP_065928                                                              16    7355
XP_005256527                                                           16    7355
NP_000375                                                              15    9605
NP_689637                                                              15    9605
NP_149082                                                              15    9605
NP_653306                                                              15    9605
NP_001243888                                                           15    9605
NP_148977                                                              15    9605
NP_001277                                                              15    9605
NP_006187                                                              15    9605
NP_001273488                                                           15    9605
NP_061938                                                              15    9605
XP_005262298                                                           15    9605
XP_006721263                                                           15    9605

>NP_001167558
          Length = 1423

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EDINASLEM 9
           EDINAS EM
Sbjct: 209 EDINASPEM 217



>NP_858045
          Length = 1424

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EDINASLEM 9
           EDINAS EM
Sbjct: 209 EDINASPEM 217



>NP_006525
          Length = 1420

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EDINASLEM 9
           EDINAS EM
Sbjct: 209 EDINASPEM 217



>NP_001167559
          Length = 1415

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EDINASLEM 9
           EDINAS EM
Sbjct: 209 EDINASPEM 217



>NP_690850
          Length = 1714

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EDINASLEM 9
           ED+N  LEM
Sbjct: 476 EDLNTMLEM 484



>NP_006556
          Length = 727

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  EDINASLEM 9
          +D+N+S++M
Sbjct: 52 QDVNSSVQM 60



>NP_005302
          Length = 594

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  DINASLE 8
          D+NASLE
Sbjct: 58 DMNASLE 64



>NP_001001549
          Length = 548

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  DINASLE 8
          D+NASLE
Sbjct: 58 DMNASLE 64



>XP_005255832
          Length = 725

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  EDINASLEM 9
          +D+N+S++M
Sbjct: 52 QDVNSSVQM 60



>XP_006715767
          Length = 588

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  DINASLE 8
          D+NASLE
Sbjct: 52 DMNASLE 58



>XP_006715766
          Length = 594

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  DINASLE 8
          D+NASLE
Sbjct: 58 DMNASLE 64



>XP_005271818
          Length = 594

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  DINASLE 8
          D+NASLE
Sbjct: 58 DMNASLE 64



>XP_005271817
          Length = 594

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  DINASLE 8
          D+NASLE
Sbjct: 58 DMNASLE 64



>NP_001171989
          Length = 186

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EDINASLEM 9
           +DIN+ +EM
Sbjct: 175 DDINSRVEM 183



>NP_001171991
          Length = 154

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EDINASLEM 9
           +DIN+ +EM
Sbjct: 143 DDINSRVEM 151



>NP_001171990
          Length = 218

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EDINASLEM 9
           +DIN+ +EM
Sbjct: 207 DDINSRVEM 215



>NP_932766
          Length = 269

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EDINASLEM 9
           +DIN+ +EM
Sbjct: 258 DDINSRVEM 266



>NP_055464
          Length = 953

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EDINASLE 8
           ED+ ASLE
Sbjct: 114 EDMEASLE 121



>NP_065928
          Length = 4427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EDINASLEM 9
            EDI  SL+M
Sbjct: 1514 EDIQKSLDM 1522



>XP_005256527
          Length = 4427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EDINASLEM 9
            EDI  SL+M
Sbjct: 1514 EDIQKSLDM 1522



>NP_000375
          Length = 4563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    EDINASLEM 9
            ED+N  L+M
Sbjct: 2414 EDVNKFLDM 2422



>NP_689637
          Length = 1135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EDINASLE 8
           ED+ ASLE
Sbjct: 832 EDLAASLE 839



>NP_149082
          Length = 474

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EDINASLE 8
           +D+N SLE
Sbjct: 252 QDVNYSLE 259



>NP_653306
          Length = 435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EDINASLE 8
           EDI+ SLE
Sbjct: 394 EDIHMSLE 401



>NP_001243888
          Length = 847

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EDINASLE 8
           ED+N+S++
Sbjct: 397 EDVNSSIK 404



>NP_148977
          Length = 500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EDINASLE 8
           +D+N SLE
Sbjct: 278 QDVNYSLE 285



>NP_001277
          Length = 869

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EDINASLE 8
           ED+N+S++
Sbjct: 419 EDVNSSIK 426



>NP_006187
          Length = 356

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  EDINASL 7
          EDIN+S+
Sbjct: 81 EDINSSM 87



>NP_001273488
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EDINASLE 8
           EDI+ SLE
Sbjct: 212 EDIHMSLE 219



>NP_061938
          Length = 386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EDINASLE 8
           ED+ ASLE
Sbjct: 131 EDVLASLE 138



>XP_005262298
          Length = 858

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EDINASLE 8
           ED+ ASLE
Sbjct: 555 EDLAASLE 562



>XP_006721263
          Length = 260

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  EDINASLE 8
          ED+ ASLE
Sbjct: 5  EDVLASLE 12



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.120    0.282 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 382,249
Number of extensions: 733
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)