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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              20     299
XP_006713721                                                           20     299
XP_006712935                                                           16    5631
XP_006712934                                                           16    5631
XP_006712933                                                           16    5631
NP_852118                                                              16    5631
NP_001004135                                                           15    9605
NP_004465                                                              15    9605

>NP_006209
          Length = 1068

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ECQIMSSAK 9
           EC+IMSSAK
Sbjct: 768 ECRIMSSAK 776



>XP_006713721
          Length = 1068

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ECQIMSSAK 9
           EC+IMSSAK
Sbjct: 768 ECRIMSSAK 776



>XP_006712935
          Length = 977

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   CQIMSSAK 9
           CQI +SAK
Sbjct: 260 CQIEASAK 267



>XP_006712934
          Length = 1583

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   CQIMSSAK 9
           CQI +SAK
Sbjct: 156 CQIEASAK 163



>XP_006712933
          Length = 1687

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   CQIMSSAK 9
           CQI +SAK
Sbjct: 260 CQIEASAK 267



>NP_852118
          Length = 1684

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   CQIMSSAK 9
           CQI +SAK
Sbjct: 257 CQIEASAK 264



>NP_001004135
          Length = 310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   CQIMSSAK 9
           CQIMS  K
Sbjct: 178 CQIMSVFK 185



>NP_004465
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  CQIMSS 7
          C+IMSS
Sbjct: 8  CEIMSS 13



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.112    0.303 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 418,989
Number of extensions: 213
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)