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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005912                                                              18    1135
XP_005248577                                                           18    1135
XP_005248576                                                           18    1135
NP_036424                                                              16    5631
NP_001269263                                                           16    5631
NP_001269264                                                           16    5631
NP_001245257                                                           16    5631
XP_006722776                                                           16    5631
NP_000254                                                              15    9605
NP_004932                                                              15    9605
XP_005257171                                                           15    9605
XP_005257170                                                           15    9605

>NP_005912
          Length = 1512

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EAVAIAMAM 9
            EA+AIAMAM
Sbjct: 1187 EALAIAMAM 1195



>XP_005248577
          Length = 1349

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EAVAIAMAM 9
            EA+AIAMAM
Sbjct: 1024 EALAIAMAM 1032



>XP_005248576
          Length = 1375

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EAVAIAMAM 9
            EA+AIAMAM
Sbjct: 1050 EALAIAMAM 1058



>NP_036424
          Length = 1136

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EAVAIAMA 8
           EAVA+A+A
Sbjct: 881 EAVAVALA 888



>NP_001269263
          Length = 771

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EAVAIAMA 8
           EAVA+A+A
Sbjct: 516 EAVAVALA 523



>NP_001269264
          Length = 1019

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EAVAIAMA 8
           EAVA+A+A
Sbjct: 764 EAVAVALA 771



>NP_001245257
          Length = 1152

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EAVAIAMA 8
           EAVA+A+A
Sbjct: 897 EAVAVALA 904



>XP_006722776
          Length = 1164

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EAVAIAMA 8
           EAVA+A+A
Sbjct: 909 EAVAVALA 916



>NP_000254
          Length = 743

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  EAVAIAMAM 9
          EAVA+A A+
Sbjct: 2  EAVAVAAAV 10



>NP_004932
          Length = 1220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2 AVAIAMA 8
         AVA+AMA
Sbjct: 2 AVAVAMA 8



>XP_005257171
          Length = 1181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2 AVAIAMA 8
         AVA+AMA
Sbjct: 2 AVAVAMA 8



>XP_005257170
          Length = 1218

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2 AVAIAMA 8
         AVA+AMA
Sbjct: 2 AVAVAMA 8



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.119    0.266 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 142,368
Number of extensions: 136
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)