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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_066997                                                              16    5631
NP_006744                                                              16    5631
NP_689493                                                              16    7355
NP_071898                                                              16    7355
XP_005245071                                                           16    7355
NP_001704                                                              15    9605
NP_705833                                                              15    9605
XP_005247174                                                           15    9605

>NP_066997
          Length = 923

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EATTKQE 7
           EATT+QE
Sbjct: 486 EATTQQE 492



>NP_006744
          Length = 361

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EATTKQEVV 9
           EAT  QEVV
Sbjct: 173 EATEAQEVV 181



>NP_689493
          Length = 431

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EATTKQEVV 9
           + TTK EVV
Sbjct: 385 DRTTKNEVV 393



>NP_071898
          Length = 459

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ATTKQEVV 9
           ATTKQ+ V
Sbjct: 187 ATTKQKAV 194



>XP_005245071
          Length = 430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EATTKQEVV 9
           + TTK EVV
Sbjct: 384 DRTTKNEVV 392



>NP_001704
          Length = 406

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   EATTKQEV 8
           EATT+Q++
Sbjct: 387 EATTEQQL 394



>NP_705833
          Length = 2970

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    EATTKQEV 8
            E+T KQEV
Sbjct: 2304 ESTYKQEV 2311



>XP_005247174
          Length = 2679

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    EATTKQEV 8
            E+T KQEV
Sbjct: 2013 ESTYKQEV 2020



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.289    0.104    0.230 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 415,151
Number of extensions: 421
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)