Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     665
XP_005262424                                                           19     665
NP_001172010                                                           17    2528
NP_001172004                                                           17    2528
NP_000065                                                              17    3301
NP_002151                                                              16    5631
NP_001123488                                                           16    5631
NP_001123489                                                           16    5631
NP_001170                                                              16    5631
XP_005252032                                                           16    5631
XP_006717164                                                           16    5631
XP_005252031                                                           16    5631
XP_005252030                                                           16    5631
XP_006717163                                                           16    5631
XP_006717162                                                           16    5631
XP_006717161                                                           16    5631
XP_006717160                                                           16    5631
XP_005252029                                                           16    5631
XP_006717159                                                           16    5631
XP_005263062                                                           16    5631
XP_005263061                                                           16    5631
XP_005263060                                                           16    5631
XP_005263059                                                           16    5631
XP_006714283                                                           16    5631
XP_005263056                                                           16    5631
XP_006714282                                                           16    5631
XP_006714281                                                           16    5631
XP_005263054                                                           16    5631
NP_001192273                                                           15    9605
NP_001703                                                              15    9605
NP_001020083                                                           15    9605
NP_001186434                                                           15    9605
NP_001186794                                                           15    9605
NP_001186795                                                           15    9605
NP_078980                                                              15    9605
NP_001157888                                                           15    9605
NP_899067                                                              15    9605
NP_775871                                                              15    9605
NP_001268436                                                           15    9605
NP_542403                                                              15    9605
NP_001186435                                                           15    9605
XP_006724703                                                           15    9605
XP_005260812                                                           15    9605
XP_006723651                                                           15    9605
XP_005260811                                                           15    9605
XP_005260810                                                           15    9605
XP_005260809                                                           15    9605
XP_005260808                                                           15    9605
XP_005260807                                                           15    9605
>NP_722576
          Length = 3080

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EASSKTTIT 9
            EASS+TTIT
Sbjct: 2106 EASSRTTIT 2114
>XP_005262424
          Length = 2875

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EASSKTTIT 9
            EASS+TTIT
Sbjct: 1901 EASSRTTIT 1909
>NP_001172010
          Length = 516

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EASSKTTIT 9
           EAS +TTIT
Sbjct: 437 EASKETTIT 445
>NP_001172004
          Length = 537

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EASSKTTIT 9
           EAS +TTIT
Sbjct: 458 EASKETTIT 466
>NP_000065
          Length = 261

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   EASSKTT 7
           EASSKTT
Sbjct: 129 EASSKTT 135
>NP_002151
          Length = 2201

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EASSKTTIT 9
           +A++KTT+T
Sbjct: 945 QATTKTTLT 953
>NP_001123488
          Length = 389

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EASSKTTI 8
           EASSKT +
Sbjct: 154 EASSKTVV 161
>NP_001123489
          Length = 367

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EASSKTTI 8
           EASSKT +
Sbjct: 154 EASSKTVV 161
>NP_001170
          Length = 378

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EASSKTTI 8
           EASSKT +
Sbjct: 154 EASSKTVV 161
>XP_005252032
          Length = 1564

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EASSKTTIT 9
           +A++KTT+T
Sbjct: 945 QATTKTTLT 953
>XP_006717164
          Length = 1655

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EASSKTTIT 9
           +A++KTT+T
Sbjct: 945 QATTKTTLT 953
>XP_005252031
          Length = 1655

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EASSKTTIT 9
           +A++KTT+T
Sbjct: 945 QATTKTTLT 953
>XP_005252030
          Length = 1837

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EASSKTTIT 9
           +A++KTT+T
Sbjct: 945 QATTKTTLT 953
>XP_006717163
          Length = 1928

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EASSKTTIT 9
           +A++KTT+T
Sbjct: 945 QATTKTTLT 953
>XP_006717162
          Length = 1928

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EASSKTTIT 9
           +A++KTT+T
Sbjct: 945 QATTKTTLT 953
>XP_006717161
          Length = 2019

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EASSKTTIT 9
           +A++KTT+T
Sbjct: 945 QATTKTTLT 953
>XP_006717160
          Length = 2110

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EASSKTTIT 9
           +A++KTT+T
Sbjct: 945 QATTKTTLT 953
>XP_005252029
          Length = 2110

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EASSKTTIT 9
           +A++KTT+T
Sbjct: 945 QATTKTTLT 953
>XP_006717159
          Length = 2293

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EASSKTTIT 9
           +A++KTT+T
Sbjct: 945 QATTKTTLT 953
>XP_005263062
          Length = 353

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EASSKTTI 8
           EASSKT +
Sbjct: 154 EASSKTVV 161
>XP_005263061
          Length = 356

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EASSKTTI 8
           EASSKT +
Sbjct: 154 EASSKTVV 161
>XP_005263060
          Length = 357

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EASSKTTI 8
           EASSKT +
Sbjct: 154 EASSKTVV 161
>XP_005263059
          Length = 363

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EASSKTTI 8
           EASSKT +
Sbjct: 154 EASSKTVV 161
>XP_006714283
          Length = 368

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EASSKTTI 8
           EASSKT +
Sbjct: 154 EASSKTVV 161
>XP_005263056
          Length = 378

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EASSKTTI 8
           EASSKT +
Sbjct: 154 EASSKTVV 161
>XP_006714282
          Length = 379

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EASSKTTI 8
           EASSKT +
Sbjct: 154 EASSKTVV 161
>XP_006714281
          Length = 390

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EASSKTTI 8
           EASSKT +
Sbjct: 154 EASSKTVV 161
>XP_005263054
          Length = 400

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EASSKTTI 8
           EASSKT +
Sbjct: 154 EASSKTVV 161
>NP_001192273
          Length = 468

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SSKTTIT 9
           +SKTT+T
Sbjct: 332 ASKTTVT 338
>NP_001703
          Length = 526

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SSKTTIT 9
           +SKTT+T
Sbjct: 332 ASKTTVT 338
>NP_001020083
          Length = 464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SSKTTIT 9
           +SKTT+T
Sbjct: 332 ASKTTVT 338
>NP_001186434
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EASSKTT 7
           +ASSKTT
Sbjct: 237 KASSKTT 243
>NP_001186794
          Length = 1266

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SKTTIT 9
          SKTTIT
Sbjct: 31 SKTTIT 36
>NP_001186795
          Length = 1392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SKTTIT 9
          SKTTIT
Sbjct: 31 SKTTIT 36
>NP_078980
          Length = 1317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SKTTIT 9
          SKTTIT
Sbjct: 31 SKTTIT 36
>NP_001157888
          Length = 154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  EASSKTT 7
          EASS+TT
Sbjct: 9  EASSETT 15
>NP_899067
          Length = 518

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EASSKTT 7
           +ASSKTT
Sbjct: 199 KASSKTT 205
>NP_775871
          Length = 8384

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    EASSKTT 7
            EAS KTT
Sbjct: 8085 EASDKTT 8091
>NP_001268436
          Length = 518

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EASSKTT 7
           +ASSKTT
Sbjct: 199 KASSKTT 205
>NP_542403
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EASSKTT 7
           +ASSKTT
Sbjct: 240 KASSKTT 246
>NP_001186435
          Length = 518

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EASSKTT 7
           +ASSKTT
Sbjct: 199 KASSKTT 205
>XP_006724703
          Length = 154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  EASSKTT 7
          EASS+TT
Sbjct: 9  EASSETT 15
>XP_005260812
          Length = 1277

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SKTTIT 9
          SKTTIT
Sbjct: 31 SKTTIT 36
>XP_006723651
          Length = 1287

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SKTTIT 9
          SKTTIT
Sbjct: 31 SKTTIT 36
>XP_005260811
          Length = 1298

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SKTTIT 9
          SKTTIT
Sbjct: 31 SKTTIT 36
>XP_005260810
          Length = 1328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SKTTIT 9
          SKTTIT
Sbjct: 31 SKTTIT 36
>XP_005260809
          Length = 1832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SKTTIT 9
          SKTTIT
Sbjct: 31 SKTTIT 36
>XP_005260808
          Length = 1872

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SKTTIT 9
          SKTTIT
Sbjct: 31 SKTTIT 36
>XP_005260807
          Length = 1883

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SKTTIT 9
          SKTTIT
Sbjct: 31 SKTTIT 36
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.278   0.0906    0.192 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 237,778
Number of extensions: 226
Number of successful extensions: 49
Number of sequences better than 10000.0: 49
Number of HSP's gapped: 49
Number of HSP's successfully gapped: 49
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.7 bits)
S2: 28 (15.4 bits)

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