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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           18    1135
NP_000170                                                              18    1135
NP_112484                                                              17    4312
NP_001230455                                                           17    4312
NP_001177809                                                           17    4312
XP_005274446                                                           17    4312
XP_005271297                                                           17    4312
XP_006711000                                                           17    4312
XP_005271296                                                           17    4312
NP_149976                                                              16    7355
NP_006166                                                              15    9605
NP_001248392                                                           15    9605
NP_932326                                                              15    9605
XP_005269924                                                           15    9605
XP_005269922                                                           15    9605
XP_006717933                                                           15    9605
XP_005269921                                                           15    9605

>NP_001268421
          Length = 1230

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  EASARASRE 9
          +ASARASRE
Sbjct: 22 KASARASRE 30



>NP_000170
          Length = 1360

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  EASARASRE 9
          +ASARASRE
Sbjct: 22 KASARASRE 30



>NP_112484
          Length = 247

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  EASARASRE 9
          EA ARA+RE
Sbjct: 46 EALARAARE 54



>NP_001230455
          Length = 272

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  EASARASRE 9
          EA ARA+RE
Sbjct: 46 EALARAARE 54



>NP_001177809
          Length = 277

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  EASARASRE 9
          EA ARA+RE
Sbjct: 46 EALARAARE 54



>XP_005274446
          Length = 415

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   EASARASR 8
           E SARASR
Sbjct: 372 ETSARASR 379



>XP_005271297
          Length = 268

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  EASARASRE 9
          EA ARA+RE
Sbjct: 46 EALARAARE 54



>XP_006711000
          Length = 289

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  EASARASRE 9
          EA ARA+RE
Sbjct: 46 EALARAARE 54



>XP_005271296
          Length = 302

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  EASARASRE 9
          EA ARA+RE
Sbjct: 46 EALARAARE 54



>NP_149976
          Length = 112

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  EASARASRE 9
          +A ARA+RE
Sbjct: 89 QARARAARE 97



>NP_006166
          Length = 1695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SARASRE 9
            +ARASRE
Sbjct: 1503 TARASRE 1509



>NP_001248392
          Length = 1731

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SARASRE 9
            +ARASRE
Sbjct: 1538 TARASRE 1544



>NP_932326
          Length = 1730

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SARASRE 9
            +ARASRE
Sbjct: 1538 TARASRE 1544



>XP_005269924
          Length = 1627

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SARASRE 9
            +ARASRE
Sbjct: 1434 TARASRE 1440



>XP_005269922
          Length = 1695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SARASRE 9
            +ARASRE
Sbjct: 1502 TARASRE 1508



>XP_006717933
          Length = 1696

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SARASRE 9
            +ARASRE
Sbjct: 1503 TARASRE 1509



>XP_005269921
          Length = 1696

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SARASRE 9
            +ARASRE
Sbjct: 1503 TARASRE 1509



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.288   0.0954    0.204 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 174,402
Number of extensions: 245
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)