Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              19     665
NP_005913                                                              19     665
XP_005267046                                                           19     665
XP_005267045                                                           19     665
NP_001229285                                                           17    3301
NP_001229286                                                           17    3301
NP_055490                                                              17    3301
NP_004820                                                              17    3301
NP_001138929                                                           17    3301
NP_001138930                                                           17    3301
NP_689916                                                              17    3301
XP_006715953                                                           17    3301
XP_006715952                                                           17    3301
XP_005250250                                                           17    3301
XP_006715951                                                           17    3301
XP_005250248                                                           17    3301
XP_006711121                                                           17    3301
XP_006711120                                                           17    3301
XP_006711119                                                           17    3301
NP_443070                                                              16    5631
NP_000029                                                              16    7355
NP_001120983                                                           16    7355
NP_001120982                                                           16    7355
NP_958815                                                              16    7355
NP_004512                                                              16    7355
NP_005947                                                              16    7355
NP_001010903                                                           16    7355
XP_005255486                                                           16    7355
XP_005255485                                                           16    7355
XP_005255484                                                           16    7355
XP_006720966                                                           16    7355
NP_006286                                                              15    9605
XP_005275347                                                           15    9605
XP_005275078                                                           15    9605
XP_005272926                                                           15    9605
XP_005274943                                                           15    9605
XP_005249419                                                           15    9605

>NP_006715
          Length = 1558

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EARERASRA 9
           EARERAS+A
Sbjct: 807 EARERASKA 815



>NP_005913
          Length = 1608

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EARERASRA 9
           EARERAS+A
Sbjct: 807 EARERASKA 815



>XP_005267046
          Length = 1554

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EARERASRA 9
           EARERAS+A
Sbjct: 807 EARERASKA 815



>XP_005267045
          Length = 1604

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EARERASRA 9
           EARERAS+A
Sbjct: 807 EARERASKA 815



>NP_001229285
          Length = 209

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   RERASRA 9
           RERASRA
Sbjct: 189 RERASRA 195



>NP_001229286
          Length = 192

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   RERASRA 9
           RERASRA
Sbjct: 172 RERASRA 178



>NP_055490
          Length = 2017

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    EARERASR 8
            EARERA R
Sbjct: 1973 EARERAHR 1980



>NP_004820
          Length = 304

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   RERASRA 9
           RERASRA
Sbjct: 284 RERASRA 290



>NP_001138929
          Length = 303

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   RERASRA 9
           RERASRA
Sbjct: 283 RERASRA 289



>NP_001138930
          Length = 287

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   RERASRA 9
           RERASRA
Sbjct: 267 RERASRA 273



>NP_689916
          Length = 1584

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    EARERASRA 9
            EA E+ASRA
Sbjct: 1154 EAAEKASRA 1162



>XP_006715953
          Length = 1584

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    EARERASRA 9
            EA E+ASRA
Sbjct: 1154 EAAEKASRA 1162



>XP_006715952
          Length = 1584

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    EARERASRA 9
            EA E+ASRA
Sbjct: 1154 EAAEKASRA 1162



>XP_005250250
          Length = 1584

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    EARERASRA 9
            EA E+ASRA
Sbjct: 1154 EAAEKASRA 1162



>XP_006715951
          Length = 1584

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    EARERASRA 9
            EA E+ASRA
Sbjct: 1154 EAAEKASRA 1162



>XP_005250248
          Length = 1584

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    EARERASRA 9
            EA E+ASRA
Sbjct: 1154 EAAEKASRA 1162



>XP_006711121
          Length = 1945

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    EARERASR 8
            EARERA R
Sbjct: 1901 EARERAHR 1908



>XP_006711120
          Length = 1972

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    EARERASR 8
            EARERA R
Sbjct: 1935 EARERAHR 1942



>XP_006711119
          Length = 1979

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    EARERASR 8
            EARERA R
Sbjct: 1935 EARERAHR 1942



>NP_443070
          Length = 414

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ARERASRA 9
           AR+RASR+
Sbjct: 349 ARDRASRS 356



>NP_000029
          Length = 2843

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EARERASRA 9
           EAR RAS A
Sbjct: 378 EARARASAA 386



>NP_001120983
          Length = 2825

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EARERASRA 9
           EAR RAS A
Sbjct: 360 EARARASAA 368



>NP_001120982
          Length = 2843

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EARERASRA 9
           EAR RAS A
Sbjct: 378 EARARASAA 386



>NP_958815
          Length = 371

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ARERASR 8
           +RERASR
Sbjct: 327 SRERASR 333



>NP_004512
          Length = 963

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EARERASR 8
           EA+E ASR
Sbjct: 883 EAKENASR 890



>NP_005947
          Length = 935

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EARERAS 7
           EA+ERAS
Sbjct: 260 EAKERAS 266



>NP_001010903
          Length = 652

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EARERASR 8
           E RE+ASR
Sbjct: 114 EPREKASR 121



>XP_005255486
          Length = 285

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ARERASR 8
           +RERASR
Sbjct: 241 SRERASR 247



>XP_005255485
          Length = 285

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ARERASR 8
           +RERASR
Sbjct: 241 SRERASR 247



>XP_005255484
          Length = 318

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ARERASR 8
           +RERASR
Sbjct: 274 SRERASR 280



>XP_006720966
          Length = 318

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ARERASR 8
           +RERASR
Sbjct: 274 SRERASR 280



>NP_006286
          Length = 1264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   EARERASR 8
           EARE+A++
Sbjct: 762 EAREKAAK 769



>XP_005275347
          Length = 1265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   EARERASR 8
           EARE+A++
Sbjct: 763 EAREKAAK 770



>XP_005275078
          Length = 1265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   EARERASR 8
           EARE+A++
Sbjct: 763 EAREKAAK 770



>XP_005272926
          Length = 1265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   EARERASR 8
           EARE+A++
Sbjct: 763 EAREKAAK 770



>XP_005274943
          Length = 1265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   EARERASR 8
           EARE+A++
Sbjct: 763 EAREKAAK 770



>XP_005249419
          Length = 1265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   EARERASR 8
           EARE+A++
Sbjct: 763 EAREKAAK 770



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.109    0.249 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 317,351
Number of extensions: 316
Number of successful extensions: 37
Number of sequences better than 10000.0: 37
Number of HSP's gapped: 37
Number of HSP's successfully gapped: 37
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)