Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001136098                                                           20     390
NP_000360                                                              20     390
NP_001018046                                                           20     390
XP_005268096                                                           20     390
XP_006720308                                                           20     390
XP_005268094                                                           20     390
NP_000224                                                              17    3301
NP_776183                                                              16    7355
XP_005259653                                                           16    7355
XP_005246532                                                           16    7355
XP_006712507                                                           16    7355
XP_006712506                                                           16    7355
XP_006712505                                                           16    7355
XP_006712504                                                           16    7355
XP_005246531                                                           16    7355
NP_064555                                                              16    7355
NP_001123214                                                           15    9605
NP_115970                                                              15    9605
NP_001124335                                                           15    9605
NP_006200                                                              15    9605
NP_001035181                                                           15    9605
NP_714913                                                              15    9605
XP_006716650                                                           15    9605
XP_006716649                                                           15    9605
XP_006716648                                                           15    9605
XP_006716647                                                           15    9605
XP_006715863                                                           15    9605
XP_005249952                                                           15    9605
XP_005249951                                                           15    9605
XP_005262943                                                           15    9605
XP_005262942                                                           15    9605
NP_570603                                                              15    9605
NP_055018                                                              15    9605
>NP_001136098
          Length = 274

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EALTLKLYN 9
           E LTLKLYN
Sbjct: 178 ETLTLKLYN 186
>NP_000360
          Length = 764

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EALTLKLYN 9
           E LTLKLYN
Sbjct: 178 ETLTLKLYN 186
>NP_001018046
          Length = 253

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EALTLKLYN 9
           E LTLKLYN
Sbjct: 178 ETLTLKLYN 186
>XP_005268096
          Length = 253

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EALTLKLYN 9
           E LTLKLYN
Sbjct: 178 ETLTLKLYN 186
>XP_006720308
          Length = 274

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EALTLKLYN 9
           E LTLKLYN
Sbjct: 178 ETLTLKLYN 186
>XP_005268094
          Length = 764

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EALTLKLYN 9
           E LTLKLYN
Sbjct: 178 ETLTLKLYN 186
>NP_000224
          Length = 699

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EALTLKLY 8
           E++TLKLY
Sbjct: 175 ESVTLKLY 182
>NP_776183
          Length = 530

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALTLKLYN 9
           EAL L +YN
Sbjct: 102 EALLLGIYN 110
>XP_005259653
          Length = 562

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALTLKLYN 9
           EALT  +YN
Sbjct: 420 EALTRVIYN 428
>XP_005246532
          Length = 504

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  EALTLKLYN 9
          EAL L +YN
Sbjct: 20 EALLLGIYN 28
>XP_006712507
          Length = 586

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALTLKLYN 9
           EAL L +YN
Sbjct: 102 EALLLGIYN 110
>XP_006712506
          Length = 586

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALTLKLYN 9
           EAL L +YN
Sbjct: 102 EALLLGIYN 110
>XP_006712505
          Length = 586

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALTLKLYN 9
           EAL L +YN
Sbjct: 102 EALLLGIYN 110
>XP_006712504
          Length = 586

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALTLKLYN 9
           EAL L +YN
Sbjct: 102 EALLLGIYN 110
>XP_005246531
          Length = 586

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALTLKLYN 9
           EAL L +YN
Sbjct: 102 EALLLGIYN 110
>NP_064555
          Length = 563

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALTLKLYN 9
           EALT  +YN
Sbjct: 420 EALTRVIYN 428
>NP_001123214
          Length = 344

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  ALTLKLYN 9
          A+T+KLY+
Sbjct: 28 AVTVKLYS 35
>NP_115970
          Length = 521

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALTLKLYN 9
           EAL L +YN
Sbjct: 102 EALLLGVYN 110
>NP_001124335
          Length = 888

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EALTLKLY 8
           E LTLK Y
Sbjct: 873 EILTLKTY 880
>NP_006200
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EALTLKLY 8
           E LTLK Y
Sbjct: 900 EILTLKTY 907
>NP_001035181
          Length = 863

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EALTLKLY 8
           E LTLK Y
Sbjct: 848 EILTLKTY 855
>NP_714913
          Length = 293

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   EALTLKLYN 9
           EAL   LYN
Sbjct: 222 EALKFHLYN 230
>XP_006716650
          Length = 911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EALTLKLY 8
           E LTLK Y
Sbjct: 896 EILTLKTY 903
>XP_006716649
          Length = 936

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EALTLKLY 8
           E LTLK Y
Sbjct: 921 EILTLKTY 928
>XP_006716648
          Length = 936

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EALTLKLY 8
           E LTLK Y
Sbjct: 921 EILTLKTY 928
>XP_006716647
          Length = 940

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EALTLKLY 8
           E LTLK Y
Sbjct: 925 EILTLKTY 932
>XP_006715863
          Length = 252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALTLKLYN 9
           EAL L +YN
Sbjct: 102 EALLLGVYN 110
>XP_005249952
          Length = 406

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALTLKLYN 9
           EAL L +YN
Sbjct: 102 EALLLGVYN 110
>XP_005249951
          Length = 419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALTLKLYN 9
           EAL L +YN
Sbjct: 102 EALLLGVYN 110
>XP_005262943
          Length = 293

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   EALTLKLYN 9
           EAL   LYN
Sbjct: 222 EALKFHLYN 230
>XP_005262942
          Length = 305

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   EALTLKLYN 9
           EAL   LYN
Sbjct: 234 EALKFHLYN 242
>NP_570603
          Length = 955

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EALTLKL 7
           +ALTLKL
Sbjct: 829 QALTLKL 835
>NP_055018
          Length = 977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EALTLKL 7
           +ALTLKL
Sbjct: 851 QALTLKL 857
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.132    0.338 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 413,450
Number of extensions: 523
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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