Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000213                                                              18    1135
NP_001087241                                                           18    1135
XP_005265799                                                           18    1135
XP_005265798                                                           18    1135
XP_005265797                                                           18    1135
NP_005202                                                              18    1482
NP_001275634                                                           18    1482
NP_006197                                                              17    3301
NP_002600                                                              17    3301
XP_005268521                                                           17    3301
XP_006714104                                                           17    3301
XP_005265800                                                           17    3301
XP_006714103                                                           17    3301
XP_006714102                                                           17    3301
NP_001035275                                                           17    4312
XP_006723530                                                           17    4312
XP_006723529                                                           17    4312
NP_002011                                                              16    5631
NP_891555                                                              16    5631
NP_002244                                                              16    5631
NP_775821                                                              16    5631
XP_005251455                                                           16    5631
XP_005251456                                                           16    5631
XP_005251454                                                           16    5631
NP_996898                                                              15    9605
NP_002010                                                              15    9605
NP_001166126                                                           15    9605
NP_060730                                                              15    9605
NP_002733                                                              15    9605
NP_001139754                                                           15    9605
XP_005268081                                                           15    9605
XP_005267916                                                           15    9605
XP_005247321                                                           15    9605
XP_006713623                                                           15    9605
NP_003127                                                              15    9605
>NP_000213
          Length = 976

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EALMSKLKV 9
           EALMS+LKV
Sbjct: 635 EALMSELKV 643
>NP_001087241
          Length = 972

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EALMSKLKV 9
           EALMS+LKV
Sbjct: 631 EALMSELKV 639
>XP_005265799
          Length = 973

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EALMSKLKV 9
           EALMS+LKV
Sbjct: 632 EALMSELKV 640
>XP_005265798
          Length = 976

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EALMSKLKV 9
           EALMS+LKV
Sbjct: 636 EALMSELKV 644
>XP_005265797
          Length = 977

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EALMSKLKV 9
           EALMS+LKV
Sbjct: 636 EALMSELKV 644
>NP_005202
          Length = 972

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   EALMSKLKV 9
           EALMS+LK+
Sbjct: 628 EALMSELKI 636
>NP_001275634
          Length = 972

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   EALMSKLKV 9
           EALMS+LK+
Sbjct: 628 EALMSELKI 636
>NP_006197
          Length = 1089

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EALMSKLKV 9
           +ALMS+LK+
Sbjct: 639 QALMSELKI 647
>NP_002600
          Length = 1106

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EALMSKLKV 9
           +ALMS+LK+
Sbjct: 646 QALMSELKI 654
>XP_005268521
          Length = 1042

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EALMSKLKV 9
           +ALMS+LK+
Sbjct: 582 QALMSELKI 590
>XP_006714104
          Length = 832

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EALMSKLKV 9
           +ALMS+LK+
Sbjct: 664 QALMSELKI 672
>XP_005265800
          Length = 1089

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EALMSKLKV 9
           +ALMS+LK+
Sbjct: 639 QALMSELKI 647
>XP_006714103
          Length = 1111

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EALMSKLKV 9
           +ALMS+LK+
Sbjct: 661 QALMSELKI 669
>XP_006714102
          Length = 1114

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   EALMSKLKV 9
           +ALMS+LK+
Sbjct: 664 QALMSELKI 672
>NP_001035275
          Length = 523

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EALMSKLK 8
           EALM+K+K
Sbjct: 110 EALMTKIK 117
>XP_006723530
          Length = 470

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  EALMSKLK 8
          EALM+K+K
Sbjct: 57 EALMTKIK 64
>XP_006723529
          Length = 523

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EALMSKLK 8
           EALM+K+K
Sbjct: 110 EALMTKIK 117
>NP_002011
          Length = 1298

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ALMSKLKV 9
           ALMS+LK+
Sbjct: 892 ALMSELKI 899
>NP_891555
          Length = 1363

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ALMSKLKV 9
           ALMS+LK+
Sbjct: 892 ALMSELKI 899
>NP_002244
          Length = 1356

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ALMSKLKV 9
           ALMS+LK+
Sbjct: 881 ALMSELKI 888
>NP_775821
          Length = 1326

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EALMSKLKV 9
           EAL+S LKV
Sbjct: 521 EALISTLKV 529
>XP_005251455
          Length = 1334

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EALMSKLKV 9
           EAL+S LKV
Sbjct: 529 EALISTLKV 537
>XP_005251456
          Length = 1334

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EALMSKLKV 9
           EAL+S LKV
Sbjct: 529 EALISTLKV 537
>XP_005251454
          Length = 1334

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EALMSKLKV 9
           EAL+S LKV
Sbjct: 529 EALISTLKV 537
>NP_996898
          Length = 795

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ALMSKLK 8
           ALMSK+K
Sbjct: 476 ALMSKVK 482
>NP_002010
          Length = 1338

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   EALMSKLKV 9
           +ALM++LK+
Sbjct: 873 KALMTELKI 881
>NP_001166126
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EALMSKLK 8
           EALM+K K
Sbjct: 176 EALMTKTK 183
>NP_060730
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EALMSKLK 8
           EALM+K K
Sbjct: 110 EALMTKTK 117
>NP_002733
          Length = 912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LMSKLKV 9
           LMSK+KV
Sbjct: 263 LMSKVKV 269
>NP_001139754
          Length = 455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EALMSKLK 8
           EAL+ KLK
Sbjct: 267 EALIEKLK 274
>XP_005268081
          Length = 447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EALMSKLK 8
           EAL+ KLK
Sbjct: 316 EALIEKLK 323
>XP_005267916
          Length = 920

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LMSKLKV 9
           LMSK+KV
Sbjct: 271 LMSKVKV 277
>XP_005247321
          Length = 746

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ALMSKLK 8
           ALMSK+K
Sbjct: 476 ALMSKVK 482
>XP_006713623
          Length = 778

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ALMSKLK 8
           ALMSK+K
Sbjct: 459 ALMSKVK 465
>NP_003127
          Length = 504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EALMSKLK 8
           EAL+ KLK
Sbjct: 316 EALIEKLK 323
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.122    0.272 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 242,432
Number of extensions: 309
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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