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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055099                                                              17    4312
NP_001014839                                                           17    4312
NP_001014841                                                           17    4312
NP_001073944                                                           16    5631
XP_005246602                                                           16    5631
XP_006712584                                                           16    5631
XP_005246601                                                           16    5631
XP_005246600                                                           16    5631
NP_001191109                                                           16    7355
NP_113618                                                              16    7355
NP_060334                                                              16    7355
XP_006725320                                                           16    7355
XP_006725319                                                           16    7355
XP_005256868                                                           16    7355
XP_006721649                                                           16    7355
XP_006716665                                                           16    7355
NP_116236                                                              16    7355
NP_056094                                                              15    9605
XP_006719364                                                           15    9605
XP_006719363                                                           15    9605

>NP_055099
          Length = 729

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   ALKVAARL 9
           ALK+AARL
Sbjct: 281 ALKLAARL 288



>NP_001014839
          Length = 729

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   ALKVAARL 9
           ALK+AARL
Sbjct: 281 ALKLAARL 288



>NP_001014841
          Length = 712

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   ALKVAARL 9
           ALK+AARL
Sbjct: 264 ALKLAARL 271



>NP_001073944
          Length = 761

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EALKVAARL 9
           EALK+AA +
Sbjct: 449 EALKIAANV 457



>XP_005246602
          Length = 651

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EALKVAARL 9
           EALK+AA +
Sbjct: 449 EALKIAANV 457



>XP_006712584
          Length = 657

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EALKVAARL 9
           EALK+AA +
Sbjct: 449 EALKIAANV 457



>XP_005246601
          Length = 761

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EALKVAARL 9
           EALK+AA +
Sbjct: 449 EALKIAANV 457



>XP_005246600
          Length = 761

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EALKVAARL 9
           EALK+AA +
Sbjct: 449 EALKIAANV 457



>NP_001191109
          Length = 292

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  EALKVAARL 9
          EAL++AA L
Sbjct: 79 EALEIAANL 87



>NP_113618
          Length = 401

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EALKVAAR 8
           EA+KVA R
Sbjct: 288 EAMKVAVR 295



>NP_060334
          Length = 995

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  ALKVAARL 9
          ALKVAA L
Sbjct: 85 ALKVAAHL 92



>XP_006725320
          Length = 373

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EALKVAAR 8
           EA+KVA R
Sbjct: 288 EAMKVAVR 295



>XP_006725319
          Length = 399

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EALKVAAR 8
           EA+KVA R
Sbjct: 286 EAMKVAVR 293



>XP_005256868
          Length = 373

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EALKVAAR 8
           EA+KVA R
Sbjct: 288 EAMKVAVR 295



>XP_006721649
          Length = 399

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EALKVAAR 8
           EA+KVA R
Sbjct: 286 EAMKVAVR 293



>XP_006716665
          Length = 943

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  ALKVAARL 9
          ALKVAA L
Sbjct: 85 ALKVAAHL 92



>NP_116236
          Length = 380

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EALKVAARL 9
           EAL++AA L
Sbjct: 111 EALEIAANL 119



>NP_056094
          Length = 761

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  EALKVAARL 9
          +ALK  ARL
Sbjct: 75 DALKAVARL 83



>XP_006719364
          Length = 924

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALKVAARL 9
           +ALK  ARL
Sbjct: 238 DALKAVARL 246



>XP_006719363
          Length = 944

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALKVAARL 9
           +ALK  ARL
Sbjct: 238 DALKAVARL 246



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.128    0.293 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 197,263
Number of extensions: 143
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)