Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245200                                                           19     869
NP_000240                                                              19     869
NP_001161089                                                           19     869
XP_005265218                                                           19     869
NP_958934                                                              16    5631
NP_005536                                                              16    5631
NP_060848                                                              16    5631
NP_001129494                                                           16    7355
NP_001185896                                                           16    7355
NP_000903                                                              16    7355
NP_001269833                                                           16    7355
NP_001131145                                                           16    7355
NP_703194                                                              16    7355
NP_060090                                                              16    7355
NP_036350                                                              16    7355
NP_004507                                                              16    7355
NP_060857                                                              16    7355
NP_001164608                                                           16    7355
NP_620711                                                              16    7355
XP_006722805                                                           16    7355
XP_005252142                                                           16    7355
XP_005252140                                                           16    7355
XP_005251309                                                           16    7355
XP_005247007                                                           16    7355
NP_001128527                                                           15    9605
NP_001244957                                                           15    9605
NP_001055                                                              15    9605
NP_001138775                                                           15    9605
NP_653287                                                              15    9605
NP_057191                                                              15    9605
NP_001252541                                                           15    9605
NP_057368                                                              15    9605
NP_996882                                                              15    9605
NP_004506                                                              15    9605
NP_001254738                                                           15    9605
NP_066013                                                              15    9605
XP_006726250                                                           15    9605
XP_006726210                                                           15    9605
XP_006726112                                                           15    9605
XP_006726013                                                           15    9605
XP_006725925                                                           15    9605
XP_006725825                                                           15    9605
XP_006725698                                                           15    9605
XP_006714540                                                           15    9605
XP_006713382                                                           15    9605
>NP_001245200
          Length = 687

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EALASISH 8
           EALASISH
Sbjct: 102 EALASISH 109
>NP_000240
          Length = 756

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EALASISH 8
           EALASISH
Sbjct: 102 EALASISH 109
>NP_001161089
          Length = 658

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  EALASISH 8
          EALASISH
Sbjct: 4  EALASISH 11
>XP_005265218
          Length = 687

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EALASISH 8
           EALASISH
Sbjct: 102 EALASISH 109
>NP_958934
          Length = 428

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  ALASISH 8
          ALAS+SH
Sbjct: 93 ALASLSH 99
>NP_005536
          Length = 428

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  ALASISH 8
          ALAS+SH
Sbjct: 93 ALASLSH 99
>NP_060848
          Length = 374

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LASISHA 9
           LAS+SHA
Sbjct: 200 LASLSHA 206
>NP_001129494
          Length = 964

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  ALASISH 8
          +LASISH
Sbjct: 12 SLASISH 18
>NP_001185896
          Length = 857

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  ALASISH 8
          +LASISH
Sbjct: 12 SLASISH 18
>NP_000903
          Length = 380

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALASISHA 9
           EAL S SH+
Sbjct: 297 EALGSTSHS 305
>NP_001269833
          Length = 291

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALASISHA 9
           EAL S SH+
Sbjct: 208 EALGSTSHS 216
>NP_001131145
          Length = 706

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALASISHA 9
           ALAS+ HA
Sbjct: 206 ALASLRHA 213
>NP_703194
          Length = 690

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALASISHA 9
           ALAS+ HA
Sbjct: 206 ALASLRHA 213
>NP_060090
          Length = 898

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALASISHA 9
           ALAS+ HA
Sbjct: 206 ALASLRHA 213
>NP_036350
          Length = 894

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALASISHA 9
           ALAS+ HA
Sbjct: 206 ALASLRHA 213
>NP_004507
          Length = 702

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALASISHA 9
           ALAS+ HA
Sbjct: 206 ALASLRHA 213
>NP_060857
          Length = 672

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALASISHA 9
           ALAS+ HA
Sbjct: 198 ALASLRHA 205
>NP_001164608
          Length = 658

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALASISHA 9
           ALAS+ HA
Sbjct: 184 ALASLRHA 191
>NP_620711
          Length = 737

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALASISHA 9
           A+ASI HA
Sbjct: 693 AIASIRHA 700
>XP_006722805
          Length = 855

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALASISHA 9
           ALAS+ HA
Sbjct: 206 ALASLRHA 213
>XP_005252142
          Length = 619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALASISHA 9
           ALAS+ HA
Sbjct: 198 ALASLRHA 205
>XP_005252140
          Length = 672

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALASISHA 9
           ALAS+ HA
Sbjct: 198 ALASLRHA 205
>XP_005251309
          Length = 366

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EALASISHA 9
           EAL S SH+
Sbjct: 283 EALGSTSHS 291
>XP_005247007
          Length = 696

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALASISHA 9
           A+ASI HA
Sbjct: 652 AIASIRHA 659
>NP_001128527
          Length = 623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EALASISHA 9
           +ALA + HA
Sbjct: 323 QALAKLGHA 331
>NP_001244957
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EALASISHA 9
           +ALA + HA
Sbjct: 331 QALAKLGHA 339
>NP_001055
          Length = 623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EALASISHA 9
           +ALA + HA
Sbjct: 323 QALAKLGHA 331
>NP_001138775
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EALASISH 8
           EAL S SH
Sbjct: 286 EALTSYSH 293
>NP_653287
          Length = 706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EALASISH 8
           EAL S SH
Sbjct: 280 EALTSYSH 287
>NP_057191
          Length = 1074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EALASISHA 9
           +ALA++ HA
Sbjct: 901 DALAALRHA 909
>NP_001252541
          Length = 2371

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LASISH 8
           LASISH
Sbjct: 970 LASISH 975
>NP_057368
          Length = 2376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LASISH 8
           LASISH
Sbjct: 975 LASISH 980
>NP_996882
          Length = 1551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LASISH 8
           LASISH
Sbjct: 975 LASISH 980
>NP_004506
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALASISHA 9
           ALA+I HA
Sbjct: 201 ALAAIRHA 208
>NP_001254738
          Length = 352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALASISHA 9
           ALA+I HA
Sbjct: 163 ALAAIRHA 170
>NP_066013
          Length = 1250

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EALASISH 8
           EAL S++H
Sbjct: 225 EALLSVAH 232
>XP_006726250
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EALASISH 8
           EAL S SH
Sbjct: 190 EALTSYSH 197
>XP_006726210
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EALASISH 8
           EAL S SH
Sbjct: 190 EALTSYSH 197
>XP_006726112
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EALASISH 8
           EAL S SH
Sbjct: 190 EALTSYSH 197
>XP_006726013
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EALASISH 8
           EAL S SH
Sbjct: 190 EALTSYSH 197
>XP_006725925
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EALASISH 8
           EAL S SH
Sbjct: 190 EALTSYSH 197
>XP_006725825
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EALASISH 8
           EAL S SH
Sbjct: 190 EALTSYSH 197
>XP_006725698
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EALASISH 8
           EAL S SH
Sbjct: 190 EALTSYSH 197
>XP_006714540
          Length = 1030

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EALASISHA 9
           +ALA++ HA
Sbjct: 857 DALAALRHA 865
>XP_006713382
          Length = 630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EALASISHA 9
           +ALA + HA
Sbjct: 323 QALAKLGHA 331
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.109    0.260 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 405,668
Number of extensions: 519
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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