Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005219                                                              20     509
NP_001276866                                                           17    2528
NP_004439                                                              17    2528
NP_001276865                                                           17    2528
NP_001005862                                                           17    2528
XP_005257197                                                           17    2528
XP_006721828                                                           17    2528
NP_001931                                                              17    4312
NP_001007027                                                           17    4312
NP_060549                                                              17    4312
NP_112598                                                              17    4312
NP_001157745                                                           16    5631
NP_001035904                                                           16    5631
NP_596870                                                              16    5631
NP_848561                                                              16    5631
NP_001074010                                                           16    5631
XP_005254311                                                           16    5631
XP_006720508                                                           16    5631
XP_006720507                                                           16    5631
XP_005254310                                                           16    5631
XP_005254309                                                           16    5631
XP_005254306                                                           16    5631
XP_005254305                                                           16    5631
XP_005254304                                                           16    5631
XP_005254303                                                           16    5631
XP_006720506                                                           16    5631
XP_005254302                                                           16    5631
XP_005254301                                                           16    5631
XP_006720505                                                           16    5631
XP_005254300                                                           16    5631
NP_003104                                                              16    7355
NP_001073860                                                           16    7355
NP_001243353                                                           16    7355
NP_000846                                                              16    7355
NP_000250                                                              16    7355
NP_001135967                                                           16    7355
NP_004220                                                              16    7355
NP_001193631                                                           16    7355
NP_001193630                                                           16    7355
XP_005272758                                                           16    7355
XP_005272757                                                           16    7355
XP_005272756                                                           16    7355
XP_005254460                                                           16    7355
XP_005254457                                                           16    7355
XP_005254455                                                           16    7355
XP_005254454                                                           16    7355
NP_066564                                                              15    9605
NP_055855                                                              15    9605
NP_062548                                                              15    9605
NP_689884                                                              15    9605
NP_001177162                                                           15    9605
NP_001171471                                                           15    9605
NP_660304                                                              15    9605
XP_006726201                                                           15    9605
XP_006721881                                                           15    9605
XP_006721880                                                           15    9605
>NP_005219
          Length = 1210

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EAISPKANK 9
           EA SPKANK
Sbjct: 749 EATSPKANK 757
>NP_001276866
          Length = 1055

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EAISPKANK 9
           E  SPKANK
Sbjct: 757 ENTSPKANK 765
>NP_004439
          Length = 1255

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EAISPKANK 9
           E  SPKANK
Sbjct: 757 ENTSPKANK 765
>NP_001276865
          Length = 1240

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EAISPKANK 9
           E  SPKANK
Sbjct: 742 ENTSPKANK 750
>NP_001005862
          Length = 1225

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EAISPKANK 9
           E  SPKANK
Sbjct: 727 ENTSPKANK 735
>XP_005257197
          Length = 1225

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EAISPKANK 9
           E  SPKANK
Sbjct: 727 ENTSPKANK 735
>XP_006721828
          Length = 1336

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EAISPKANK 9
           E  SPKANK
Sbjct: 838 ENTSPKANK 846
>NP_001931
          Length = 1190

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  EAISPKANK 9
          EA +PK NK
Sbjct: 61 EASTPKVNK 69
>NP_001007027
          Length = 1190

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  EAISPKANK 9
          EA +PK NK
Sbjct: 61 EASTPKVNK 69
>NP_060549
          Length = 995

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AISPKANK 9
           ++SP+ANK
Sbjct: 636 SVSPEANK 643
>NP_112598
          Length = 5088

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   ISPKANK 9
           I PKANK
Sbjct: 654 IDPKANK 660
>NP_001157745
          Length = 3065

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EAISPKAN 8
            EA+ P+AN
Sbjct: 1999 EAVDPEAN 2006
>NP_001035904
          Length = 218

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EAISPKANK 9
           EAI PK N+
Sbjct: 143 EAIPPKMNQ 151
>NP_596870
          Length = 5604

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EAISPKANK 9
            EAI PK N+
Sbjct: 5172 EAIEPKDNE 5180
>NP_848561
          Length = 254

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EAISPKANK 9
           EAI PK N+
Sbjct: 179 EAIPPKMNQ 187
>NP_001074010
          Length = 2856

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EAISPKAN 8
            EA+ P+AN
Sbjct: 1790 EAVDPEAN 1797
>XP_005254311
          Length = 1627

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EAISPKAN 8
           EA+ P+AN
Sbjct: 560 EAVDPEAN 567
>XP_006720508
          Length = 2658

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EAISPKAN 8
            EA+ P+AN
Sbjct: 1591 EAVDPEAN 1598
>XP_006720507
          Length = 2763

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EAISPKAN 8
            EA+ P+AN
Sbjct: 1696 EAVDPEAN 1703
>XP_005254310
          Length = 2906

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EAISPKAN 8
            EA+ P+AN
Sbjct: 1839 EAVDPEAN 1846
>XP_005254309
          Length = 2956

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EAISPKAN 8
            EA+ P+AN
Sbjct: 1889 EAVDPEAN 1896
>XP_005254306
          Length = 3066

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EAISPKAN 8
            EA+ P+AN
Sbjct: 1999 EAVDPEAN 2006
>XP_005254305
          Length = 3114

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EAISPKAN 8
            EA+ P+AN
Sbjct: 2048 EAVDPEAN 2055
>XP_005254304
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EAISPKAN 8
            EA+ P+AN
Sbjct: 2048 EAVDPEAN 2055
>XP_005254303
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EAISPKAN 8
            EA+ P+AN
Sbjct: 2048 EAVDPEAN 2055
>XP_006720506
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EAISPKAN 8
            EA+ P+AN
Sbjct: 2048 EAVDPEAN 2055
>XP_005254302
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EAISPKAN 8
            EA+ P+AN
Sbjct: 2048 EAVDPEAN 2055
>XP_005254301
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EAISPKAN 8
            EA+ P+AN
Sbjct: 2048 EAVDPEAN 2055
>XP_006720505
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EAISPKAN 8
            EA+ P+AN
Sbjct: 2048 EAVDPEAN 2055
>XP_005254300
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    EAISPKAN 8
            EA+ P+AN
Sbjct: 2048 EAVDPEAN 2055
>NP_003104
          Length = 879

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  EAISPKANK 9
          E ISP AN+
Sbjct: 15 ECISPVANE 23
>NP_001073860
          Length = 885

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  EAISPKANK 9
          E ISP AN+
Sbjct: 15 ECISPVANE 23
>NP_001243353
          Length = 763

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   EAISPKAN 8
           E +SPK N
Sbjct: 365 EIVSPKVN 372
>NP_000846
          Length = 732

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   EAISPKAN 8
           E +SPK N
Sbjct: 365 EIVSPKVN 372
>NP_000250
          Length = 1855

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EAISPKANK 9
            EAI PK +K
Sbjct: 1278 EAIQPKDDK 1286
>NP_001135967
          Length = 1828

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EAISPKANK 9
            EAI PK +K
Sbjct: 1278 EAIQPKDDK 1286
>NP_004220
          Length = 1454

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    AISPKANK 9
            A+SPK N+
Sbjct: 1252 ALSPKTNQ 1259
>NP_001193631
          Length = 480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  EAISPKANK 9
          E ISP AN+
Sbjct: 15 ECISPVANE 23
>NP_001193630
          Length = 688

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  EAISPKANK 9
          E ISP AN+
Sbjct: 15 ECISPVANE 23
>XP_005272758
          Length = 1338

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    AISPKANK 9
            A+SPK N+
Sbjct: 1136 ALSPKTNQ 1143
>XP_005272757
          Length = 1410

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    AISPKANK 9
            A+SPK N+
Sbjct: 1208 ALSPKTNQ 1215
>XP_005272756
          Length = 1443

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    AISPKANK 9
            A+SPK N+
Sbjct: 1252 ALSPKTNQ 1259
>XP_005254460
          Length = 1481

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EAISPKANK 9
            EAI PK +K
Sbjct: 1278 EAIQPKDDK 1286
>XP_005254457
          Length = 1853

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EAISPKANK 9
            EAI PK +K
Sbjct: 1278 EAIQPKDDK 1286
>XP_005254455
          Length = 1880

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EAISPKANK 9
            EAI PK +K
Sbjct: 1278 EAIQPKDDK 1286
>XP_005254454
          Length = 1880

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EAISPKANK 9
            EAI PK +K
Sbjct: 1278 EAIQPKDDK 1286
>NP_066564
          Length = 837

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AISPKANK 9
           +++PKAN+
Sbjct: 507 SLTPKANR 514
>NP_055855
          Length = 2098

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AISPKANK 9
           ++SP+AN+
Sbjct: 327 SVSPQANR 334
>NP_062548
          Length = 455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   ISPKAN 8
           +SPKAN
Sbjct: 269 LSPKAN 274
>NP_689884
          Length = 451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AISPKANK 9
           ++SP+AN+
Sbjct: 230 SVSPQANR 237
>NP_001177162
          Length = 91

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  ISPKANK 9
          +SP ANK
Sbjct: 44 VSPTANK 50
>NP_001171471
          Length = 548

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AISPKANK 9
           ++SP+AN+
Sbjct: 327 SVSPQANR 334
>NP_660304
          Length = 116

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  ISPKANK 9
          +SP ANK
Sbjct: 69 VSPTANK 75
>XP_006726201
          Length = 245

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  ISPKAN 8
          +SPKAN
Sbjct: 59 LSPKAN 64
>XP_006721881
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AISPKANK 9
           +++PKAN+
Sbjct: 146 SLTPKANR 153
>XP_006721880
          Length = 838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AISPKANK 9
           +++PKAN+
Sbjct: 507 SLTPKANR 514
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.291    0.110    0.273 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 675,064
Number of extensions: 749
Number of successful extensions: 56
Number of sequences better than 10000.0: 56
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)

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