Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              19     869
NP_444254                                                              19     869
NP_444253                                                              19     869
NP_444255                                                              19     869
NP_444260                                                              19     869
XP_005247551                                                           19     869
XP_005247550                                                           19     869
XP_005247549                                                           19     869
NP_444259                                                              17    2528
XP_006713712                                                           17    2528
NP_001269828                                                           17    4312
NP_001269829                                                           17    4312
NP_001269830                                                           17    4312
NP_001093867                                                           17    4312
NP_008954                                                              17    4312
XP_006712269                                                           17    4312
XP_006712268                                                           17    4312
XP_005246303                                                           17    4312
NP_064506                                                              16    5631
NP_817123                                                              16    5631
NP_001127925                                                           16    5631
XP_006717797                                                           16    7355
NP_777567                                                              16    7355
NP_056005                                                              16    7355
NP_003267                                                              15    9605
NP_783195                                                              15    9605
NP_006522                                                              15    9605
NP_848611                                                              15    9605
NP_001073920                                                           15    9605
XP_005266612                                                           15    9605
XP_006719933                                                           15    9605
XP_006719932                                                           15    9605
XP_005266610                                                           15    9605
XP_005266609                                                           15    9605
XP_005266608                                                           15    9605
XP_005266607                                                           15    9605
XP_005266606                                                           15    9605
XP_005266605                                                           15    9605
XP_005266604                                                           15    9605
XP_005266603                                                           15    9605
>NP_444256
          Length = 1794

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EADVSQAFL 9
            E DVSQAFL
Sbjct: 1669 EEDVSQAFL 1677
>NP_444254
          Length = 1845

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EADVSQAFL 9
            E DVSQAFL
Sbjct: 1720 EEDVSQAFL 1728
>NP_444253
          Length = 1914

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EADVSQAFL 9
            E DVSQAFL
Sbjct: 1789 EEDVSQAFL 1797
>NP_444255
          Length = 1863

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EADVSQAFL 9
            E DVSQAFL
Sbjct: 1738 EEDVSQAFL 1746
>NP_444260
          Length = 154

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  EADVSQAFL 9
          E DVSQAFL
Sbjct: 29 EEDVSQAFL 37
>XP_005247551
          Length = 1855

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EADVSQAFL 9
            E DVSQAFL
Sbjct: 1730 EEDVSQAFL 1738
>XP_005247550
          Length = 1856

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EADVSQAFL 9
            E DVSQAFL
Sbjct: 1731 EEDVSQAFL 1739
>XP_005247549
          Length = 1924

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EADVSQAFL 9
            E DVSQAFL
Sbjct: 1799 EEDVSQAFL 1807
>NP_444259
          Length = 153

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3  DVSQAFL 9
          DVSQAFL
Sbjct: 30 DVSQAFL 36
>XP_006713712
          Length = 1923

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3    DVSQAFL 9
            DVSQAFL
Sbjct: 1800 DVSQAFL 1806
>NP_001269828
          Length = 858

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EADVSQAFL 9
           E DVS AFL
Sbjct: 441 EDDVSMAFL 449
>NP_001269829
          Length = 840

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EADVSQAFL 9
           E DVS AFL
Sbjct: 423 EDDVSMAFL 431
>NP_001269830
          Length = 791

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EADVSQAFL 9
           E DVS AFL
Sbjct: 374 EDDVSMAFL 382
>NP_001093867
          Length = 867

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EADVSQAFL 9
           E DVS AFL
Sbjct: 450 EDDVSMAFL 458
>NP_008954
          Length = 1011

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EADVSQAFL 9
           E DVS AFL
Sbjct: 594 EDDVSMAFL 602
>XP_006712269
          Length = 838

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EADVSQAFL 9
           E DVS AFL
Sbjct: 421 EDDVSMAFL 429
>XP_006712268
          Length = 840

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EADVSQAFL 9
           E DVS AFL
Sbjct: 423 EDDVSMAFL 431
>XP_005246303
          Length = 993

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EADVSQAFL 9
           E DVS AFL
Sbjct: 576 EDDVSMAFL 584
>NP_064506
          Length = 1516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EADVSQAFL 9
           E D+S+AF+
Sbjct: 547 EFDISEAFI 555
>NP_817123
          Length = 1040

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EADVSQAFL 9
           +AD++ AFL
Sbjct: 771 DADINMAFL 779
>NP_001127925
          Length = 1059

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EADVSQAFL 9
           +AD++ AFL
Sbjct: 790 DADINMAFL 798
>XP_006717797
          Length = 238

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ADVSQAFL 9
           AD+ QA+L
Sbjct: 128 ADIKQAYL 135
>NP_777567
          Length = 417

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EADVSQA 7
           E+DVSQA
Sbjct: 348 ESDVSQA 354
>NP_056005
          Length = 260

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ADVSQAFL 9
           AD+ QA+L
Sbjct: 128 ADIKQAYL 135
>NP_003267
          Length = 694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EADVSQA 7
           +AD+SQA
Sbjct: 594 QADISQA 600
>NP_783195
          Length = 833

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EADVSQAF 8
           E D SQAF
Sbjct: 608 EGDKSQAF 615
>NP_006522
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EADVSQAF 8
           E D SQAF
Sbjct: 599 EGDKSQAF 606
>NP_848611
          Length = 746

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   EADVSQ 6
           EADVSQ
Sbjct: 359 EADVSQ 364
>NP_001073920
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   EADVSQAF 8
           EAD+ Q F
Sbjct: 112 EADIHQGF 119
>XP_005266612
          Length = 805

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EADVSQAF 8
           E D SQAF
Sbjct: 580 EGDKSQAF 587
>XP_006719933
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EADVSQAF 8
           E D SQAF
Sbjct: 599 EGDKSQAF 606
>XP_006719932
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EADVSQAF 8
           E D SQAF
Sbjct: 599 EGDKSQAF 606
>XP_005266610
          Length = 833

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EADVSQAF 8
           E D SQAF
Sbjct: 608 EGDKSQAF 615
>XP_005266609
          Length = 843

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EADVSQAF 8
           E D SQAF
Sbjct: 656 EGDKSQAF 663
>XP_005266608
          Length = 844

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EADVSQAF 8
           E D SQAF
Sbjct: 656 EGDKSQAF 663
>XP_005266607
          Length = 844

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EADVSQAF 8
           E D SQAF
Sbjct: 619 EGDKSQAF 626
>XP_005266606
          Length = 847

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EADVSQAF 8
           E D SQAF
Sbjct: 622 EGDKSQAF 629
>XP_005266605
          Length = 852

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EADVSQAF 8
           E D SQAF
Sbjct: 627 EGDKSQAF 634
>XP_005266604
          Length = 862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EADVSQAF 8
           E D SQAF
Sbjct: 637 EGDKSQAF 644
>XP_005266603
          Length = 881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   EADVSQAF 8
           E D SQAF
Sbjct: 656 EGDKSQAF 663
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.125    0.308 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 352,519
Number of extensions: 447
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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