Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              18    1135
NP_653080                                                              18    1135
XP_005267292                                                           18    1135
XP_005267291                                                           18    1135
XP_005267290                                                           18    1135
NP_001273762                                                           17    3301
NP_005246                                                              17    3301
XP_005272330                                                           17    3301
XP_005272329                                                           17    3301
XP_005272327                                                           17    3301
XP_006713937                                                           17    3301
XP_005272326                                                           17    3301
XP_005272325                                                           17    3301
NP_003793                                                              17    4312
XP_006726779                                                           17    4312
XP_006721689                                                           17    4312
XP_006710090                                                           17    4312
NP_001139512                                                           16    7355
NP_000162                                                              16    7355
NP_940880                                                              16    7355
NP_001171808                                                           16    7355
NP_001129663                                                           16    7355
NP_003865                                                              16    7355
NP_001171810                                                           16    7355
NP_056308                                                              16    7355
XP_005252949                                                           16    7355
XP_005252948                                                           16    7355
XP_005268469                                                           16    7355
NP_848632                                                              16    7355
NP_004553                                                              15    9605
NP_054642                                                              15    9605
NP_054643                                                              15    9605
NP_006149                                                              15    9605
NP_001132931                                                           15    9605
NP_689822                                                              15    9605
NP_078789                                                              15    9605
NP_036392                                                              15    9605
NP_001271342                                                           15    9605
NP_001271345                                                           15    9605
XP_005265541                                                           15    9605
XP_006713398                                                           15    9605
XP_006713397                                                           15    9605
XP_006713396                                                           15    9605
XP_005273140                                                           15    9605
XP_006711814                                                           15    9605
XP_005273139                                                           15    9605
XP_005273138                                                           15    9605
>NP_005091
          Length = 1782

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EAASKESE 8
           EAASKESE
Sbjct: 224 EAASKESE 231
>NP_653080
          Length = 1684

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EAASKESE 8
           EAASKESE
Sbjct: 126 EAASKESE 133
>XP_005267292
          Length = 1677

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EAASKESE 8
           EAASKESE
Sbjct: 119 EAASKESE 126
>XP_005267291
          Length = 1684

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EAASKESE 8
           EAASKESE
Sbjct: 126 EAASKESE 133
>XP_005267290
          Length = 1782

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EAASKESE 8
           EAASKESE
Sbjct: 224 EAASKESE 231
>NP_001273762
          Length = 1213

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AASKESES 9
           A+SKESES
Sbjct: 712 ASSKESES 719
>NP_005246
          Length = 1311

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AASKESES 9
           A+SKESES
Sbjct: 810 ASSKESES 817
>XP_005272330
          Length = 1140

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AASKESES 9
           A+SKESES
Sbjct: 810 ASSKESES 817
>XP_005272329
          Length = 1175

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AASKESES 9
           A+SKESES
Sbjct: 674 ASSKESES 681
>XP_005272327
          Length = 1236

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AASKESES 9
           A+SKESES
Sbjct: 810 ASSKESES 817
>XP_006713937
          Length = 1249

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AASKESES 9
           A+SKESES
Sbjct: 748 ASSKESES 755
>XP_005272326
          Length = 1262

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AASKESES 9
           A+SKESES
Sbjct: 761 ASSKESES 768
>XP_005272325
          Length = 1300

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AASKESES 9
           A+SKESES
Sbjct: 810 ASSKESES 817
>NP_003793
          Length = 1938

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    EAASKESES 9
            EAA KES S
Sbjct: 1472 EAAQKESRS 1480
>XP_006726779
          Length = 430

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EAASKESES 9
           EAAS+E ES
Sbjct: 101 EAASRELES 109
>XP_006721689
          Length = 438

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   EAASKESES 9
           EAAS+E ES
Sbjct: 101 EAASRELES 109
>XP_006710090
          Length = 255

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  EAASKESES 9
          EAAS+E ES
Sbjct: 78 EAASRELES 86
>NP_001139512
          Length = 457

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  AASKESES 9
          AASKE+E+
Sbjct: 21 AASKEAEA 28
>NP_000162
          Length = 449

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  AASKESES 9
          AASKE+E+
Sbjct: 21 AASKEAEA 28
>NP_940880
          Length = 832

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EAASKESE 8
           EAA +ESE
Sbjct: 226 EAAQRESE 233
>NP_001171808
          Length = 345

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  EAASKESE 8
          EAA K+SE
Sbjct: 18 EAAGKDSE 25
>NP_001129663
          Length = 851

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EAASKESE 8
           +AAS+ESE
Sbjct: 460 QAASQESE 467
>NP_003865
          Length = 328

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  EAASKESE 8
          EAA K+SE
Sbjct: 18 EAAGKDSE 25
>NP_001171810
          Length = 272

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  EAASKESE 8
          EAA K+SE
Sbjct: 18 EAAGKDSE 25
>NP_056308
          Length = 859

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EAASKESE 8
           +AAS+ESE
Sbjct: 460 QAASQESE 467
>XP_005252949
          Length = 1001

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EAASKESE 8
           EAAS++SE
Sbjct: 658 EAASEDSE 665
>XP_005252948
          Length = 1007

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EAASKESE 8
           EAAS++SE
Sbjct: 626 EAASEDSE 633
>XP_005268469
          Length = 307

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  AASKESES 9
          AASKE+E+
Sbjct: 21 AASKEAEA 28
>NP_848632
          Length = 1039

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EAASKESE 8
           EAAS++SE
Sbjct: 658 EAASEDSE 665
>NP_004553
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EAASKES 7
           EAASKE+
Sbjct: 404 EAASKET 410
>NP_054642
          Length = 437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EAASKES 7
           EAASKE+
Sbjct: 376 EAASKET 382
>NP_054643
          Length = 316

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EAASKES 7
           EAASKE+
Sbjct: 255 EAASKET 261
>NP_006149
          Length = 543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EAASKESE 8
           EAA +ESE
Sbjct: 496 EAAKEESE 503
>NP_001132931
          Length = 616

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AASKESES 9
           A +KESES
Sbjct: 307 ATTKESES 314
>NP_689822
          Length = 725

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AASKESES 9
           A +KESES
Sbjct: 307 ATTKESES 314
>NP_078789
          Length = 1478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EAASKESE 8
           EAAS+E E
Sbjct: 938 EAASRERE 945
>NP_036392
          Length = 578

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EAASKESES 9
           EAASKE  S
Sbjct: 361 EAASKELAS 369
>NP_001271342
          Length = 551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EAASKESES 9
           EAASKE  S
Sbjct: 334 EAASKELAS 342
>NP_001271345
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EAASKESES 9
           EAASKE  S
Sbjct: 279 EAASKELAS 287
>XP_005265541
          Length = 1080

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EAASKESE 8
           EAAS+E E
Sbjct: 938 EAASRERE 945
>XP_006713398
          Length = 1478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EAASKESE 8
           EAAS+E E
Sbjct: 938 EAASRERE 945
>XP_006713397
          Length = 1478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EAASKESE 8
           EAAS+E E
Sbjct: 938 EAASRERE 945
>XP_006713396
          Length = 1478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EAASKESE 8
           EAAS+E E
Sbjct: 938 EAASRERE 945
>XP_005273140
          Length = 560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AASKESES 9
           A +KESES
Sbjct: 142 ATTKESES 149
>XP_006711814
          Length = 603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AASKESES 9
           A +KESES
Sbjct: 185 ATTKESES 192
>XP_005273139
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AASKESES 9
           A +KESES
Sbjct: 348 ATTKESES 355
>XP_005273138
          Length = 781

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AASKESES 9
           A +KESES
Sbjct: 363 ATTKESES 370
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.249   0.0761    0.146 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 365,275
Number of extensions: 357
Number of successful extensions: 47
Number of sequences better than 10000.0: 47
Number of HSP's gapped: 47
Number of HSP's successfully gapped: 47
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 20 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (13.8 bits)
S2: 28 (15.4 bits)

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