Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              21     229
NP_001010909                                                           17    3301
XP_005275464                                                           17    3301
XP_005275463                                                           17    3301
XP_005275181                                                           17    3301
XP_005275180                                                           17    3301
NP_002652                                                              17    4312
NP_000691                                                              17    4312
NP_002194                                                              17    4312
NP_006740                                                              16    5631
NP_001244109                                                           16    5631
NP_001244110                                                           16    5631
NP_001098677                                                           16    5631
NP_002281                                                              16    5631
NP_001098676                                                           16    5631
XP_006716453                                                           16    5631
XP_005273672                                                           16    5631
XP_005273670                                                           16    5631
XP_006715544                                                           16    5631
XP_005267041                                                           16    5631
XP_006715543                                                           16    5631
XP_005267040                                                           16    5631
NP_003791                                                              16    7355
NP_001273357                                                           16    7355
NP_001273355                                                           16    7355
XP_006715652                                                           16    7355
NP_001186930                                                           15    9605
NP_077719                                                              15    9605
NP_060087                                                              15    9605
NP_000426                                                              15    9605
NP_004896                                                              15    9605
NP_001257293                                                           15    9605
NP_036322                                                              15    9605
NP_001257294                                                           15    9605
NP_000683                                                              15    9605
NP_005827                                                              15    9605
XP_005259981                                                           15    9605
XP_006713545                                                           15    9605
XP_006713544                                                           15    9605
XP_005270959                                                           15    9605
XP_005270958                                                           15    9605
XP_006710730                                                           15    9605
NP_057629                                                              15    9605
>NP_004351
          Length = 882

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DVNTYNTAI 9
           DVNTYN AI
Sbjct: 292 DVNTYNAAI 300
>NP_001010909
          Length = 566

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NTYNTAI 9
           NT+NTA+
Sbjct: 510 NTFNTAV 516
>XP_005275464
          Length = 572

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NTYNTAI 9
           NT+NTA+
Sbjct: 516 NTFNTAV 522
>XP_005275463
          Length = 626

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NTYNTAI 9
           NT+NTA+
Sbjct: 570 NTFNTAV 576
>XP_005275181
          Length = 572

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NTYNTAI 9
           NT+NTA+
Sbjct: 516 NTFNTAV 522
>XP_005275180
          Length = 596

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NTYNTAI 9
           NT+NTA+
Sbjct: 540 NTFNTAV 546
>NP_002652
          Length = 1265

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   DVNTYN 6
           D+NTYN
Sbjct: 854 DLNTYN 859
>NP_000691
          Length = 346

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DVNTYNTAI 9
           DVN +NT +
Sbjct: 217 DVNVFNTIL 225
>NP_002194
          Length = 1181

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    DVNTYNTAI 9
            ++NTYN  I
Sbjct: 1099 EINTYNPEI 1107
>NP_006740
          Length = 652

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  DVNTYNTAI 9
          DVN YN  +
Sbjct: 76 DVNLYNETV 84
>NP_001244109
          Length = 652

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  DVNTYNTAI 9
          DVN YN  +
Sbjct: 76 DVNLYNETV 84
>NP_001244110
          Length = 652

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  DVNTYNTAI 9
          DVN YN  +
Sbjct: 76 DVNLYNETV 84
>NP_001098677
          Length = 1816

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DVNTYNTAI 9
           D + YNTA+
Sbjct: 763 DSSAYNTAV 771
>NP_002281
          Length = 1816

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DVNTYNTAI 9
           D + YNTA+
Sbjct: 763 DSSAYNTAV 771
>NP_001098676
          Length = 1823

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DVNTYNTAI 9
           D + YNTA+
Sbjct: 770 DSSAYNTAV 778
>XP_006716453
          Length = 605

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  DVNTYNTAI 9
          DVN YN  +
Sbjct: 76 DVNLYNETV 84
>XP_005273672
          Length = 612

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  DVNTYNTAI 9
          DVN YN  +
Sbjct: 76 DVNLYNETV 84
>XP_005273670
          Length = 652

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  DVNTYNTAI 9
          DVN YN  +
Sbjct: 76 DVNLYNETV 84
>XP_006715544
          Length = 1810

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DVNTYNTAI 9
           D + YNTA+
Sbjct: 757 DSSAYNTAV 765
>XP_005267041
          Length = 1823

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DVNTYNTAI 9
           D + YNTA+
Sbjct: 770 DSSAYNTAV 778
>XP_006715543
          Length = 1823

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DVNTYNTAI 9
           D + YNTA+
Sbjct: 770 DSSAYNTAV 778
>XP_005267040
          Length = 1823

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DVNTYNTAI 9
           D + YNTA+
Sbjct: 770 DSSAYNTAV 778
>NP_003791
          Length = 597

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NTYNTAI 9
           N YNTA+
Sbjct: 537 NAYNTAM 543
>NP_001273357
          Length = 514

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NTYNTAI 9
           N YNTA+
Sbjct: 454 NAYNTAM 460
>NP_001273355
          Length = 574

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NTYNTAI 9
           N YNTA+
Sbjct: 514 NAYNTAM 520
>XP_006715652
          Length = 574

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NTYNTAI 9
           N YNTA+
Sbjct: 514 NAYNTAM 520
>NP_001186930
          Length = 1235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   VNTYN 6
           VNTYN
Sbjct: 279 VNTYN 283
>NP_077719
          Length = 2471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   VNTYN 6
           VNTYN
Sbjct: 279 VNTYN 283
>NP_060087
          Length = 2555

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   VNTYN 6
           VNTYN
Sbjct: 276 VNTYN 280
>NP_000426
          Length = 2321

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   VNTYN 6
           VNTYN
Sbjct: 255 VNTYN 259
>NP_004896
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1  DVNTYN 6
          D+N YN
Sbjct: 85 DINAYN 90
>NP_001257293
          Length = 912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   VNTYN 6
           VNTYN
Sbjct: 870 VNTYN 874
>NP_036322
          Length = 902

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   VNTYN 6
           VNTYN
Sbjct: 860 VNTYN 864
>NP_001257294
          Length = 801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   VNTYN 6
           VNTYN
Sbjct: 759 VNTYN 763
>NP_000683
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   VNTYN 6
           VNTYN
Sbjct: 470 VNTYN 474
>NP_005827
          Length = 137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1  DVNTYNT 7
          D+N +NT
Sbjct: 85 DINDFNT 91
>XP_005259981
          Length = 2269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   VNTYN 6
           VNTYN
Sbjct: 255 VNTYN 259
>XP_006713545
          Length = 900

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   VNTYN 6
           VNTYN
Sbjct: 858 VNTYN 862
>XP_006713544
          Length = 902

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   VNTYN 6
           VNTYN
Sbjct: 860 VNTYN 864
>XP_005270959
          Length = 2432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   VNTYN 6
           VNTYN
Sbjct: 240 VNTYN 244
>XP_005270958
          Length = 2432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   VNTYN 6
           VNTYN
Sbjct: 240 VNTYN 244
>XP_006710730
          Length = 2432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   VNTYN 6
           VNTYN
Sbjct: 240 VNTYN 244
>NP_057629
          Length = 327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DVNTYNTAI 9
           D+N ++TAI
Sbjct: 316 DMNPWSTAI 324
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.123    0.338 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 455,938
Number of extensions: 591
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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