Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001804                                                              18    1935
NP_001273663                                                           18    1935
NP_002481                                                              18    1935
NP_148980                                                              17    4312
NP_056990                                                              17    4312
XP_005270994                                                           17    4312
XP_005270993                                                           17    4312
NP_001186101                                                           16    5631
NP_002638                                                              16    5631
NP_653278                                                              16    5631
NP_945193                                                              16    5631
NP_660208                                                              16    5631
NP_003585                                                              16    5631
XP_006721771                                                           16    5631
XP_006721770                                                           16    5631
XP_005257122                                                           16    5631
XP_006714180                                                           16    5631
XP_005262841                                                           16    5631
XP_005262840                                                           16    5631
XP_005271334                                                           16    5631
XP_006711044                                                           16    5631
XP_006711043                                                           16    5631
XP_006711042                                                           16    5631
NP_001177766                                                           16    7355
NP_001128512                                                           16    7355
NP_004073                                                              16    7355
NP_075408                                                              16    7355
NP_001128513                                                           16    7355
NP_001177765                                                           16    7355
NP_059523                                                              16    7355
NP_149119                                                              16    7355
NP_001106177                                                           16    7355
NP_003209                                                              16    7355
NP_001030017                                                           16    7355
NP_004230                                                              16    7355
XP_005268271                                                           16    7355
XP_005268270                                                           16    7355
XP_006720384                                                           16    7355
XP_005251349                                                           16    7355
XP_005251348                                                           16    7355
XP_005249698                                                           16    7355
NP_001129396                                                           16    7355
NP_000337                                                              15    9605
NP_060147                                                              15    9605
NP_000250                                                              15    9605
NP_001135967                                                           15    9605
NP_055524                                                              15    9605
NP_653270                                                              15    9605
NP_001269949                                                           15    9605
NP_001269947                                                           15    9605
NP_002694                                                              15    9605
NP_071363                                                              15    9605
NP_065937                                                              15    9605
NP_005427                                                              15    9605
XP_005254459                                                           15    9605
XP_005254458                                                           15    9605
XP_005254457                                                           15    9605
XP_005254456                                                           15    9605
XP_005254455                                                           15    9605
XP_005254454                                                           15    9605
XP_005253611                                                           15    9605
XP_006719310                                                           15    9605
XP_006719309                                                           15    9605
XP_006719308                                                           15    9605
XP_005253610                                                           15    9605
XP_005271698                                                           15    9605
XP_005271696                                                           15    9605
XP_005271695                                                           15    9605
XP_005271694                                                           15    9605
XP_005271693                                                           15    9605
XP_005271690                                                           15    9605
XP_005271689                                                           15    9605
XP_005271688                                                           15    9605
XP_005271687                                                           15    9605
NP_061741                                                              15    9605
NP_114443                                                              15    9605
>NP_001804
          Length = 2701

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    DVNEAEKRV 9
            DVNE +K+V
Sbjct: 1838 DVNETQKKV 1846
>NP_001273663
          Length = 2580

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    DVNEAEKRV 9
            DVNE +K+V
Sbjct: 1813 DVNETQKKV 1821
>NP_002481
          Length = 154

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1  DVNEAEKRV 9
          D+NEA++RV
Sbjct: 50 DMNEAKRRV 58
>NP_148980
          Length = 1110

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DVNEAEKRV 9
           +VNE  KRV
Sbjct: 350 EVNETNKRV 358
>NP_056990
          Length = 1127

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DVNEAEKRV 9
           +VNE  KRV
Sbjct: 350 EVNETNKRV 358
>XP_005270994
          Length = 1134

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DVNEAEKRV 9
           +VNE  KRV
Sbjct: 350 EVNETNKRV 358
>XP_005270993
          Length = 1151

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DVNEAEKRV 9
           +VNE  KRV
Sbjct: 350 EVNETNKRV 358
>NP_001186101
          Length = 792

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DVNEAEKRV 9
           ++NE EKR+
Sbjct: 445 ELNETEKRL 453
>NP_002638
          Length = 887

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VNEAEKR 8
           +NE+EKR
Sbjct: 204 INESEKR 210
>NP_653278
          Length = 790

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DVNEAEKRV 9
           ++NE EKR+
Sbjct: 445 ELNETEKRL 453
>NP_945193
          Length = 801

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DVNEAEKRV 9
           ++NE EKR+
Sbjct: 456 ELNETEKRL 464
>NP_660208
          Length = 893

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NE EKRV
Sbjct: 467 NEVEKRV 473
>NP_003585
          Length = 1162

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NE EKRV
Sbjct: 658 NEVEKRV 664
>XP_006721771
          Length = 436

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DVNEAEKRV 9
          ++NE EKR+
Sbjct: 89 ELNETEKRL 97
>XP_006721770
          Length = 729

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DVNEAEKRV 9
           ++NE EKR+
Sbjct: 382 ELNETEKRL 390
>XP_005257122
          Length = 735

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DVNEAEKRV 9
           ++NE EKR+
Sbjct: 388 ELNETEKRL 396
>XP_006714180
          Length = 799

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NE EKRV
Sbjct: 467 NEVEKRV 473
>XP_005262841
          Length = 835

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NE EKRV
Sbjct: 467 NEVEKRV 473
>XP_005262840
          Length = 892

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NE EKRV
Sbjct: 466 NEVEKRV 472
>XP_005271334
          Length = 1161

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NE EKRV
Sbjct: 657 NEVEKRV 663
>XP_006711044
          Length = 1168

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NE EKRV
Sbjct: 664 NEVEKRV 670
>XP_006711043
          Length = 1169

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NE EKRV
Sbjct: 665 NEVEKRV 671
>XP_006711042
          Length = 1169

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NE EKRV
Sbjct: 665 NEVEKRV 671
>NP_001177766
          Length = 1271

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VNEAEKRV 9
           V EA+KRV
Sbjct: 489 VREAQKRV 496
>NP_001128512
          Length = 1253

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VNEAEKRV 9
           V EA+KRV
Sbjct: 476 VREAQKRV 483
>NP_004073
          Length = 1278

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VNEAEKRV 9
           V EA+KRV
Sbjct: 496 VREAQKRV 503
>NP_075408
          Length = 1144

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VNEAEKRV 9
           V EA+KRV
Sbjct: 362 VREAQKRV 369
>NP_001128513
          Length = 1139

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VNEAEKRV 9
           V EA+KRV
Sbjct: 362 VREAQKRV 369
>NP_001177765
          Length = 1236

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VNEAEKRV 9
           V EA+KRV
Sbjct: 459 VREAQKRV 466
>NP_059523
          Length = 439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DVNEAEKRV 9
           D+N+ E+RV
Sbjct: 334 DLNKKERRV 342
>NP_149119
          Length = 468

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   DVNEAEKR 8
           D NE EK+
Sbjct: 414 DANEVEKK 421
>NP_001106177
          Length = 715

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   DVNEAEKR 8
           D NE EK+
Sbjct: 661 DANEVEKK 668
>NP_003209
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DVNEAEKRV 9
           D+N+ E+RV
Sbjct: 314 DLNKKERRV 322
>NP_001030017
          Length = 601

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DVNEAEKRV 9
           D N AEK++
Sbjct: 560 DCNPAEKKI 568
>NP_004230
          Length = 1979

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DVNEAEKRV 9
           D+N+ +KRV
Sbjct: 535 DLNDEKKRV 543
>XP_005268271
          Length = 1537

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DVNEAEKRV 9
           D+N+ +KRV
Sbjct: 93  DLNDEKKRV 101
>XP_005268270
          Length = 1950

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DVNEAEKRV 9
           D+N+ +KRV
Sbjct: 506 DLNDEKKRV 514
>XP_006720384
          Length = 1978

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DVNEAEKRV 9
           D+N+ +KRV
Sbjct: 534 DLNDEKKRV 542
>XP_005251349
          Length = 449

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DVNEAEKRV 9
           D+N+ E+RV
Sbjct: 314 DLNKKERRV 322
>XP_005251348
          Length = 469

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DVNEAEKRV 9
           D+N+ E+RV
Sbjct: 334 DLNKKERRV 342
>XP_005249698
          Length = 1044

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NE EKR+
Sbjct: 314 NECEKRI 320
>NP_001129396
          Length = 1590

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NE EKR+
Sbjct: 860 NECEKRI 866
>NP_000337
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VNEAEKR 8
           +NE+EKR
Sbjct: 146 LNESEKR 152
>NP_060147
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DVNEAEKRV 9
           DV+E EK++
Sbjct: 530 DVHELEKKL 538
>NP_000250
          Length = 1855

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VNEAEKRV 9
            VNE E+RV
Sbjct: 1789 VNEFEERV 1796
>NP_001135967
          Length = 1828

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VNEAEKRV 9
            VNE E+RV
Sbjct: 1762 VNEFEERV 1769
>NP_055524
          Length = 3546

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   VNEAEK 7
           +NEAEK
Sbjct: 880 INEAEK 885
>NP_653270
          Length = 266

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DVNEAEK 7
          D N+AEK
Sbjct: 90 DANQAEK 96
>NP_001269949
          Length = 259

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EAEKRV 9
          EAEKRV
Sbjct: 16 EAEKRV 21
>NP_001269947
          Length = 302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EAEKRV 9
          EAEKRV
Sbjct: 16 EAEKRV 21
>NP_002694
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   DVNEAEKR 8
           D+N+ EK+
Sbjct: 216 DINDKEKK 223
>NP_071363
          Length = 298

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  EAEKRV 9
          EAEKRV
Sbjct: 16 EAEKRV 21
>NP_065937
          Length = 1077

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NEA KRV
Sbjct: 835 NEAPKRV 841
>NP_005427
          Length = 474

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DVNEAEKRV 9
           D  EAEKR+
Sbjct: 222 DKLEAEKRI 230
>XP_005254459
          Length = 1825

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VNEAEKRV 9
            VNE E+RV
Sbjct: 1759 VNEFEERV 1766
>XP_005254458
          Length = 1852

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VNEAEKRV 9
            VNE E+RV
Sbjct: 1786 VNEFEERV 1793
>XP_005254457
          Length = 1853

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VNEAEKRV 9
            VNE E+RV
Sbjct: 1787 VNEFEERV 1794
>XP_005254456
          Length = 1877

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VNEAEKRV 9
            VNE E+RV
Sbjct: 1811 VNEFEERV 1818
>XP_005254455
          Length = 1880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VNEAEKRV 9
            VNE E+RV
Sbjct: 1814 VNEFEERV 1821
>XP_005254454
          Length = 1880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VNEAEKRV 9
            VNE E+RV
Sbjct: 1814 VNEFEERV 1821
>XP_005253611
          Length = 304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DVNEAEK 7
          D N+AEK
Sbjct: 48 DANQAEK 54
>XP_006719310
          Length = 306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DVNEAEK 7
          D N+AEK
Sbjct: 90 DANQAEK 96
>XP_006719309
          Length = 314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DVNEAEK 7
          D N+AEK
Sbjct: 90 DANQAEK 96
>XP_006719308
          Length = 327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DVNEAEK 7
          D N+AEK
Sbjct: 90 DANQAEK 96
>XP_005253610
          Length = 346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DVNEAEK 7
          D N+AEK
Sbjct: 90 DANQAEK 96
>XP_005271698
          Length = 920

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NEA KRV
Sbjct: 678 NEAPKRV 684
>XP_005271696
          Length = 1045

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NEA KRV
Sbjct: 803 NEAPKRV 809
>XP_005271695
          Length = 1046

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NEA KRV
Sbjct: 804 NEAPKRV 810
>XP_005271694
          Length = 1078

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NEA KRV
Sbjct: 836 NEAPKRV 842
>XP_005271693
          Length = 1080

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NEA KRV
Sbjct: 838 NEAPKRV 844
>XP_005271690
          Length = 1118

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NEA KRV
Sbjct: 876 NEAPKRV 882
>XP_005271689
          Length = 1137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NEA KRV
Sbjct: 895 NEAPKRV 901
>XP_005271688
          Length = 1139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NEA KRV
Sbjct: 897 NEAPKRV 903
>XP_005271687
          Length = 1140

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NEAEKRV 9
           NEA KRV
Sbjct: 898 NEAPKRV 904
>NP_061741
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DVNEAEKRV 9
           D N+ E+RV
Sbjct: 798 DANKEERRV 806
>NP_114443
          Length = 813

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DVNEAEKRV 9
           D N+ E+RV
Sbjct: 798 DANKEERRV 806
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.121    0.293 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 510,565
Number of extensions: 977
Number of successful extensions: 76
Number of sequences better than 10000.0: 76
Number of HSP's gapped: 76
Number of HSP's successfully gapped: 76
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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