Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              19     869
NP_005913                                                              19     869
XP_005267046                                                           19     869
XP_005267045                                                           19     869
NP_006819                                                              17    4312
XP_006715385                                                           17    4312
NP_004577                                                              16    5631
XP_005274634                                                           16    5631
XP_006724570                                                           16    5631
XP_005274633                                                           16    5631
XP_005274632                                                           16    5631
XP_006724569                                                           16    5631
XP_005274630                                                           16    5631
XP_006724568                                                           16    5631
XP_005259877                                                           16    5631
NP_055790                                                              16    5631
NP_060871                                                              16    7355
NP_001274669                                                           16    7355
NP_002378                                                              16    7355
NP_001078846                                                           16    7355
NP_659438                                                              16    7355
XP_005272048                                                           16    7355
XP_006714680                                                           16    7355
NP_001106195                                                           16    7355
NP_001158152                                                           15    9605
NP_847898                                                              15    9605
NP_001828                                                              15    9605
NP_847899                                                              15    9605
NP_878314                                                              15    9605
NP_006101                                                              15    9605
NP_001263380                                                           15    9605
NP_001230575                                                           15    9605
XP_006713023                                                           15    9605
>NP_006715
          Length = 1558

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DTRELMAMK 9
            DT ELMAMK
Sbjct: 1314 DTGELMAMK 1322
>NP_005913
          Length = 1608

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DTRELMAMK 9
            DT ELMAMK
Sbjct: 1364 DTGELMAMK 1372
>XP_005267046
          Length = 1554

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DTRELMAMK 9
            DT ELMAMK
Sbjct: 1310 DTGELMAMK 1318
>XP_005267045
          Length = 1604

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DTRELMAMK 9
            DT ELMAMK
Sbjct: 1360 DTGELMAMK 1368
>NP_006819
          Length = 2202

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    DTRELMAMK 9
            D REL+A+K
Sbjct: 2123 DKRELIALK 2131
>XP_006715385
          Length = 1866

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    DTRELMAMK 9
            D REL+A+K
Sbjct: 1787 DKRELIALK 1795
>NP_004577
          Length = 740

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DTRELMAMK 9
           D R+L AMK
Sbjct: 92  DARQLYAMK 100
>XP_005274634
          Length = 710

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DTRELMAMK 9
          D R+L AMK
Sbjct: 63 DARQLYAMK 71
>XP_006724570
          Length = 711

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DTRELMAMK 9
          D R+L AMK
Sbjct: 64 DARQLYAMK 72
>XP_005274633
          Length = 711

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DTRELMAMK 9
          D R+L AMK
Sbjct: 63 DARQLYAMK 71
>XP_005274632
          Length = 712

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DTRELMAMK 9
          D R+L AMK
Sbjct: 64 DARQLYAMK 72
>XP_006724569
          Length = 712

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DTRELMAMK 9
          D R+L AMK
Sbjct: 64 DARQLYAMK 72
>XP_005274630
          Length = 739

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DTRELMAMK 9
           D R+L AMK
Sbjct: 92  DARQLYAMK 100
>XP_006724568
          Length = 740

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DTRELMAMK 9
           D R+L AMK
Sbjct: 92  DARQLYAMK 100
>XP_005259877
          Length = 1394

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DTRELMAMK 9
           DTR+  AMK
Sbjct: 219 DTRQRFAMK 227
>NP_055790
          Length = 1570

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DTRELMAMK 9
           DTR+  AMK
Sbjct: 395 DTRQRFAMK 403
>NP_060871
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DTRELMAMK 9
          DT+++ AMK
Sbjct: 44 DTKKMYAMK 52
>NP_001274669
          Length = 407

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DTRELMAMK 9
          DT+++ AMK
Sbjct: 44 DTKKMYAMK 52
>NP_002378
          Length = 829

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RELMAMK 9
           +ELMAMK
Sbjct: 620 QELMAMK 626
>NP_001078846
          Length = 1019

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RELMAMK 9
           +ELMAMK
Sbjct: 810 QELMAMK 816
>NP_659438
          Length = 166

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DTRELMAMK 9
          DT+++ AMK
Sbjct: 44 DTKKMYAMK 52
>XP_005272048
          Length = 851

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RELMAMK 9
           +ELMAMK
Sbjct: 620 QELMAMK 626
>XP_006714680
          Length = 1002

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RELMAMK 9
           +ELMAMK
Sbjct: 771 QELMAMK 777
>NP_001106195
          Length = 396

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DTRELMAMK 9
          DT+++ AMK
Sbjct: 44 DTKKMYAMK 52
>NP_001158152
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  DTRELMA 7
          DTR LMA
Sbjct: 46 DTRALMA 52
>NP_847898
          Length = 376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  DTRELMA 7
          DTR LMA
Sbjct: 49 DTRALMA 55
>NP_001828
          Length = 355

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  DTRELMA 7
          DTR LMA
Sbjct: 28 DTRALMA 34
>NP_847899
          Length = 355

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  DTRELMA 7
          DTR LMA
Sbjct: 28 DTRALMA 34
>NP_878314
          Length = 361

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   RELMAMK 9
           RE++AMK
Sbjct: 178 REMLAMK 184
>NP_006101
          Length = 341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DTRELMAMK 9
           +TRE +AM+
Sbjct: 220 ETRERLAMR 228
>NP_001263380
          Length = 263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  TRELMAMK 9
          TRE +AMK
Sbjct: 62 TREELAMK 69
>NP_001230575
          Length = 341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DTRELMAMK 9
           +TRE +AM+
Sbjct: 220 ETRERLAMR 228
>XP_006713023
          Length = 355

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  DTRELMA 7
          DTR LMA
Sbjct: 28 DTRALMA 34
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.124    0.310 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 346,234
Number of extensions: 381
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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