Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              19     665
XP_006716142                                                           19     665
XP_005250088                                                           19     665
XP_006716141                                                           19     665
XP_005250085                                                           19     665
XP_006716140                                                           19     665
XP_005250084                                                           19     665
XP_005250083                                                           19     665
XP_005250082                                                           19     665
NP_001073996                                                           17    4312
XP_006712602                                                           17    4312
NP_065789                                                              16    5631
NP_001136171                                                           16    7355
NP_001027466                                                           15    9605
NP_000053                                                              15    9605
NP_001073932                                                           15    9605
NP_001368                                                              15    9605
NP_840060                                                              15    9605
NP_001167543                                                           15    9605
NP_001167542                                                           15    9605
NP_955452                                                              15    9605
XP_006718966                                                           15    9605
XP_005248535                                                           15    9605
XP_006713543                                                           15    9605
>NP_733751
          Length = 4911

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DTMLNAQRS 9
            DTM NAQRS
Sbjct: 1627 DTMSNAQRS 1635
>XP_006716142
          Length = 4927

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DTMLNAQRS 9
            DTM NAQRS
Sbjct: 1644 DTMSNAQRS 1652
>XP_005250088
          Length = 4928

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DTMLNAQRS 9
            DTM NAQRS
Sbjct: 1644 DTMSNAQRS 1652
>XP_006716141
          Length = 4959

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DTMLNAQRS 9
            DTM NAQRS
Sbjct: 1644 DTMSNAQRS 1652
>XP_005250085
          Length = 4979

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DTMLNAQRS 9
            DTM NAQRS
Sbjct: 1644 DTMSNAQRS 1652
>XP_006716140
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DTMLNAQRS 9
            DTM NAQRS
Sbjct: 1644 DTMSNAQRS 1652
>XP_005250084
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DTMLNAQRS 9
            DTM NAQRS
Sbjct: 1644 DTMSNAQRS 1652
>XP_005250083
          Length = 4982

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DTMLNAQRS 9
            DTM NAQRS
Sbjct: 1643 DTMSNAQRS 1651
>XP_005250082
          Length = 4983

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DTMLNAQRS 9
            DTM NAQRS
Sbjct: 1644 DTMSNAQRS 1652
>NP_001073996
          Length = 2116

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DTMLNAQRS 9
           DT+L  QRS
Sbjct: 752 DTLLEVQRS 760
>XP_006712602
          Length = 2142

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DTMLNAQRS 9
           DT+L  QRS
Sbjct: 752 DTLLEVQRS 760
>NP_065789
          Length = 1771

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   DTMLNAQR 8
           D++LN+QR
Sbjct: 716 DSLLNSQR 723
>NP_001136171
          Length = 975

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  TMLNAQRS 9
          T+LN+Q+S
Sbjct: 22 TLLNSQKS 29
>NP_001027466
          Length = 500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DTMLNAQRS 9
           DT +NA R+
Sbjct: 234 DTFVNASRT 242
>NP_000053
          Length = 500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DTMLNAQRS 9
           DT +NA R+
Sbjct: 234 DTFVNASRT 242
>NP_001073932
          Length = 4314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    DTMLNAQR 8
            DT LNA R
Sbjct: 4184 DTFLNALR 4191
>NP_001368
          Length = 4307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    DTMLNAQR 8
            DT LNA R
Sbjct: 4177 DTFLNALR 4184
>NP_840060
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   DTMLNAQR 8
           DT LNA R
Sbjct: 148 DTFLNAWR 155
>NP_001167543
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   DTMLNAQR 8
           DT LNA R
Sbjct: 148 DTFLNAWR 155
>NP_001167542
          Length = 420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   DTMLNAQR 8
           DT LNA R
Sbjct: 148 DTFLNAWR 155
>NP_955452
          Length = 2590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TMLNAQRS 9
           ++LNAQR+
Sbjct: 800 SLLNAQRA 807
>XP_006718966
          Length = 4300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    DTMLNAQR 8
            DT LNA R
Sbjct: 4170 DTFLNALR 4177
>XP_005248535
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  DTMLNAQR 8
          DT LNA R
Sbjct: 77 DTFLNAWR 84
>XP_006713543
          Length = 2591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TMLNAQRS 9
           ++LNAQR+
Sbjct: 801 SLLNAQRA 808
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.114    0.284 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 307,317
Number of extensions: 331
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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