Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004351                                                              20     299
NP_001004725                                                           18    1135
NP_001005270                                                           17    3301
NP_919268                                                              17    3301
XP_005268803                                                           17    4312
XP_005268802                                                           17    4312
XP_005268801                                                           17    4312
XP_005268800                                                           17    4312
XP_005268799                                                           17    4312
NP_056090                                                              17    4312
NP_001004719                                                           16    5631
NP_001191227                                                           16    5631
NP_689650                                                              16    5631
NP_001005500                                                           16    5631
NP_787074                                                              16    7355
NP_001032895                                                           16    7355
XP_005269107                                                           16    7355
XP_006719588                                                           16    7355
XP_006719587                                                           16    7355
NP_001128634                                                           15    9605
NP_000745                                                              15    9605
NP_996769                                                              15    9605
NP_009294                                                              15    9605
NP_001128633                                                           15    9605
NP_055178                                                              15    9605
NP_001161052                                                           15    9605
NP_071364                                                              15    9605
NP_055663                                                              15    9605
NP_690054                                                              15    9605
NP_001264984                                                           15    9605
NP_775809                                                              15    9605
NP_001191005                                                           15    9605
NP_008975                                                              15    9605
NP_898884                                                              15    9605
XP_006724421                                                           15    9605
XP_006724420                                                           15    9605
XP_006724419                                                           15    9605
XP_006724418                                                           15    9605
XP_006724417                                                           15    9605
XP_006724416                                                           15    9605
XP_005261286                                                           15    9605
XP_005255055                                                           15    9605
XP_005255054                                                           15    9605
XP_005266395                                                           15    9605
XP_006716575                                                           15    9605
XP_005266862                                                           15    9605
XP_005266861                                                           15    9605
XP_006713666                                                           15    9605
XP_006713665                                                           15    9605
XP_006710675                                                           15    9605
XP_006710674                                                           15    9605
XP_006710673                                                           15    9605
XP_006710672                                                           15    9605
NP_001004726                                                           15    9605
>NP_004351
          Length = 882

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   DTFMEQKI 8
           DTFMEQKI
Sbjct: 514 DTFMEQKI 521
>NP_001004725
          Length = 309

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  DTFMEQKII 9
          DTF+E KII
Sbjct: 82 DTFVEHKII 90
>NP_001005270
          Length = 309

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  DTFMEQKII 9
          D+F E+KII
Sbjct: 82 DSFQEKKII 90
>NP_919268
          Length = 657

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DTFMEQKII 9
           DT  EQK++
Sbjct: 346 DTIQEQKVV 354
>XP_005268803
          Length = 851

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DTFMEQK 7
           DTF++QK
Sbjct: 388 DTFLQQK 394
>XP_005268802
          Length = 852

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DTFMEQK 7
           DTF++QK
Sbjct: 388 DTFLQQK 394
>XP_005268801
          Length = 985

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DTFMEQK 7
           DTF++QK
Sbjct: 200 DTFLQQK 206
>XP_005268800
          Length = 986

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DTFMEQK 7
           DTF++QK
Sbjct: 200 DTFLQQK 206
>XP_005268799
          Length = 1174

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DTFMEQK 7
           DTF++QK
Sbjct: 388 DTFLQQK 394
>NP_056090
          Length = 1173

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DTFMEQK 7
           DTF++QK
Sbjct: 388 DTFLQQK 394
>NP_001004719
          Length = 313

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  DTFMEQKII 9
          D F+E+KII
Sbjct: 84 DFFVERKII 92
>NP_001191227
          Length = 261

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  DTFMEQKI 8
          D FM QKI
Sbjct: 17 DLFMHQKI 24
>NP_689650
          Length = 261

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  DTFMEQKI 8
          D FM QKI
Sbjct: 17 DLFMHQKI 24
>NP_001005500
          Length = 313

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  DTFMEQKII 9
          D F+E+KII
Sbjct: 84 DFFVERKII 92
>NP_787074
          Length = 459

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   DTFMEQK 7
           D FM+QK
Sbjct: 155 DNFMQQK 161
>NP_001032895
          Length = 1334

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    DTFMEQ 6
            DTFM+Q
Sbjct: 1023 DTFMQQ 1028
>XP_005269107
          Length = 1289

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    DTFMEQ 6
            DTFM+Q
Sbjct: 1023 DTFMQQ 1028
>XP_006719588
          Length = 1334

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    DTFMEQ 6
            DTFM+Q
Sbjct: 1023 DTFMQQ 1028
>XP_006719587
          Length = 1334

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    DTFMEQ 6
            DTFM+Q
Sbjct: 1023 DTFMQQ 1028
>NP_001128634
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DTFMEQK 7
          DT+ EQK
Sbjct: 80 DTYCEQK 86
>NP_000745
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DTFMEQK 7
          DT+ EQK
Sbjct: 80 DTYCEQK 86
>NP_996769
          Length = 1299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    MEQKII 9
            MEQKII
Sbjct: 1042 MEQKII 1047
>NP_009294
          Length = 221

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DTFMEQK 7
          DT+ EQK
Sbjct: 30 DTYCEQK 36
>NP_001128633
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DTFMEQK 7
          DT+ EQK
Sbjct: 80 DTYCEQK 86
>NP_055178
          Length = 4579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1    DTFMEQKI 8
            + FM+QK+
Sbjct: 4572 ENFMQQKV 4579
>NP_001161052
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TFMEQK 7
           TF+EQK
Sbjct: 367 TFLEQK 372
>NP_071364
          Length = 708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   DTFMEQ 6
           DTF+EQ
Sbjct: 140 DTFIEQ 145
>NP_055663
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TFMEQK 7
           TF+EQK
Sbjct: 518 TFLEQK 523
>NP_690054
          Length = 703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   DTFMEQ 6
           DTF+EQ
Sbjct: 135 DTFIEQ 140
>NP_001264984
          Length = 4432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1    DTFMEQKI 8
            + FM+QK+
Sbjct: 4425 ENFMQQKV 4432
>NP_775809
          Length = 436

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  DTFMEQ 6
          DTFM+Q
Sbjct: 64 DTFMKQ 69
>NP_001191005
          Length = 311

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  DTFMEQK 7
          DT+++QK
Sbjct: 45 DTYLQQK 51
>NP_008975
          Length = 491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  DTFMEQK 7
          DT+++QK
Sbjct: 45 DTYLQQK 51
>NP_898884
          Length = 1177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DTFMEQKI 8
           DT+M QK+
Sbjct: 96  DTYMLQKL 103
>XP_006724421
          Length = 667

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   DTFMEQ 6
           DTF+EQ
Sbjct: 140 DTFIEQ 145
>XP_006724420
          Length = 673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   DTFMEQ 6
           DTF+EQ
Sbjct: 140 DTFIEQ 145
>XP_006724419
          Length = 698

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   DTFMEQ 6
           DTF+EQ
Sbjct: 140 DTFIEQ 145
>XP_006724418
          Length = 726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   DTFMEQ 6
           DTF+EQ
Sbjct: 140 DTFIEQ 145
>XP_006724417
          Length = 726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   DTFMEQ 6
           DTF+EQ
Sbjct: 140 DTFIEQ 145
>XP_006724416
          Length = 726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   DTFMEQ 6
           DTF+EQ
Sbjct: 140 DTFIEQ 145
>XP_005261286
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DTFMEQK 7
          DT+ EQK
Sbjct: 80 DTYCEQK 86
>XP_005255055
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TFMEQK 7
           TF+EQK
Sbjct: 518 TFLEQK 523
>XP_005255054
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   TFMEQK 7
           TF+EQK
Sbjct: 518 TFLEQK 523
>XP_005266395
          Length = 4588

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1    DTFMEQKI 8
            + FM+QK+
Sbjct: 4581 ENFMQQKV 4588
>XP_006716575
          Length = 515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  DTFMEQ 6
          DTFM+Q
Sbjct: 64 DTFMKQ 69
>XP_005266862
          Length = 438

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  DTFMEQK 7
          DT+++QK
Sbjct: 45 DTYLQQK 51
>XP_005266861
          Length = 491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1  DTFMEQK 7
          DT+++QK
Sbjct: 45 DTYLQQK 51
>XP_006713666
          Length = 1104

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  DTFMEQKI 8
          DT+M QK+
Sbjct: 23 DTYMLQKL 30
>XP_006713665
          Length = 1175

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DTFMEQKI 8
           DT+M QK+
Sbjct: 96  DTYMLQKL 103
>XP_006710675
          Length = 783

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MEQKII 9
           MEQKII
Sbjct: 370 MEQKII 375
>XP_006710674
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   MEQKII 9
           MEQKII
Sbjct: 721 MEQKII 726
>XP_006710673
          Length = 1420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    MEQKII 9
            MEQKII
Sbjct: 1150 MEQKII 1155
>XP_006710672
          Length = 1563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    MEQKII 9
            MEQKII
Sbjct: 1150 MEQKII 1155
>NP_001004726
          Length = 305

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  DTFMEQKII 9
          D+F+++K+I
Sbjct: 82 DSFIKRKVI 90
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.142    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 509,984
Number of extensions: 936
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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