Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              20     509
NP_004064                                                              17    4312
NP_056142                                                              16    7355
NP_003706                                                              16    7355
NP_001276978                                                           16    7355
NP_064450                                                              16    7355
XP_006714460                                                           16    7355
XP_006714459                                                           16    7355
XP_005273257                                                           16    7355
XP_006711311                                                           16    7355
XP_005245106                                                           16    7355
NP_054734                                                              16    7355
NP_001127835                                                           15    9605
NP_001182505                                                           15    9605
NP_001182507                                                           15    9605
NP_001182506                                                           15    9605
NP_001153777                                                           15    9605
NP_079136                                                              15    9605
NP_000333                                                              15    9605
XP_005257650                                                           15    9605
XP_005257649                                                           15    9605
XP_006721334                                                           15    9605
NP_849150                                                              15    9605
>NP_004251
          Length = 1208

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DTDQALLSL 9
           DTDQALL+L
Sbjct: 683 DTDQALLTL 691
>NP_004064
          Length = 646

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   TDQALLSL 9
           TDQALL L
Sbjct: 569 TDQALLML 576
>NP_056142
          Length = 1016

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   DTDQALLS 8
           DTD+ LLS
Sbjct: 750 DTDRPLLS 757
>NP_003706
          Length = 962

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DTDQALLSL 9
           D DQ +LSL
Sbjct: 915 DQDQKILSL 923
>NP_001276978
          Length = 973

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DTDQALLSL 9
           D DQ +LSL
Sbjct: 926 DQDQKILSL 934
>NP_064450
          Length = 1722

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   DTDQALLSL 9
           DT+QAL +L
Sbjct: 268 DTEQALSAL 276
>XP_006714460
          Length = 966

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DTDQALLSL 9
           D DQ +LSL
Sbjct: 919 DQDQKILSL 927
>XP_006714459
          Length = 969

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DTDQALLSL 9
           D DQ +LSL
Sbjct: 922 DQDQKILSL 930
>XP_005273257
          Length = 1726

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   DTDQALLSL 9
           DT+QAL +L
Sbjct: 268 DTEQALSAL 276
>XP_006711311
          Length = 950

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   DTDQALLS 8
           DTD+ LLS
Sbjct: 684 DTDRPLLS 691
>XP_005245106
          Length = 970

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   DTDQALLS 8
           DTD+ LLS
Sbjct: 704 DTDRPLLS 711
>NP_054734
          Length = 95

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DTDQALLSL 9
          D DQAL+ L
Sbjct: 62 DMDQALVDL 70
>NP_001127835
          Length = 1227

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   DQALLSL 9
           DQALLS+
Sbjct: 385 DQALLSV 391
>NP_001182505
          Length = 111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  DTDQALLSL 9
          D DQA+L +
Sbjct: 85 DIDQAMLDM 93
>NP_001182507
          Length = 111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  DTDQALLSL 9
          D DQA+L +
Sbjct: 85 DIDQAMLDM 93
>NP_001182506
          Length = 111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  DTDQALLSL 9
          D DQA+L +
Sbjct: 85 DIDQAMLDM 93
>NP_001153777
          Length = 473

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TDQALLS 8
           TDQ LLS
Sbjct: 433 TDQGLLS 439
>NP_079136
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TDQALLS 8
           TDQ LLS
Sbjct: 409 TDQGLLS 415
>NP_000333
          Length = 911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TDQALLSL 9
           ++QALLSL
Sbjct: 328 SEQALLSL 335
>XP_005257650
          Length = 846

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TDQALLSL 9
           ++QALLSL
Sbjct: 263 SEQALLSL 270
>XP_005257649
          Length = 911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TDQALLSL 9
           ++QALLSL
Sbjct: 328 SEQALLSL 335
>XP_006721334
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   TDQALLS 8
           TDQ LLS
Sbjct: 436 TDQGLLS 442
>NP_849150
          Length = 1445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   DTDQALL 7
           DT QALL
Sbjct: 670 DTQQALL 676
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.128    0.314 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 456,106
Number of extensions: 690
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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