Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           19     869
NP_000242                                                              19     869
XP_005264389                                                           19     869
NP_056046                                                              17    3301
NP_036433                                                              16    5631
XP_005250782                                                           16    5631
NP_001234926                                                           16    7355
NP_055680                                                              16    7355
NP_001123553                                                           16    7355
NP_002947                                                              16    7355
NP_002653                                                              16    7355
NP_937883                                                              16    7355
NP_001248351                                                           16    7355
XP_006719618                                                           16    7355
XP_006719617                                                           16    7355
XP_006719616                                                           16    7355
XP_006719615                                                           16    7355
XP_006719614                                                           16    7355
XP_005253652                                                           16    7355
XP_005253651                                                           16    7355
XP_005253650                                                           16    7355
XP_005247591                                                           16    7355
XP_005247590                                                           16    7355
NP_001136254                                                           15    9605
NP_116317                                                              15    9605
NP_690864                                                              15    9605
NP_060542                                                              15    9605
XP_005270794                                                           15    9605
NP_115536                                                              15    9605
>NP_001245210
          Length = 868

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DSQLKGVFT 9
           DSQLKGV T
Sbjct: 650 DSQLKGVST 658
>NP_000242
          Length = 934

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DSQLKGVFT 9
           DSQLKGV T
Sbjct: 716 DSQLKGVST 724
>XP_005264389
          Length = 921

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DSQLKGVFT 9
           DSQLKGV T
Sbjct: 716 DSQLKGVST 724
>NP_056046
          Length = 1436

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DSQLKGVFT 9
           DSQ++ +FT
Sbjct: 180 DSQMQSIFT 188
>NP_036433
          Length = 1455

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SQLKGVF 8
           SQLKG F
Sbjct: 418 SQLKGTF 424
>XP_005250782
          Length = 1441

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SQLKGVF 8
           SQLKG F
Sbjct: 418 SQLKGTF 424
>NP_001234926
          Length = 1438

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LKGVFT 9
           LKG+FT
Sbjct: 114 LKGIFT 119
>NP_055680
          Length = 1401

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    DSQLKGVFT 9
            DSQL+ +FT
Sbjct: 1053 DSQLRLLFT 1061
>NP_001123553
          Length = 1036

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DSQLKGV 7
           DS+LKG+
Sbjct: 545 DSKLKGI 551
>NP_002947
          Length = 1427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LKGVFT 9
           LKG+FT
Sbjct: 114 LKGIFT 119
>NP_002653
          Length = 1074

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DSQLKGV 7
           DS+LKG+
Sbjct: 545 DSKLKGI 551
>NP_937883
          Length = 1392

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LKGVFT 9
           LKG+FT
Sbjct: 114 LKGIFT 119
>NP_001248351
          Length = 560

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DSQLKGVF 8
           DS++KG F
Sbjct: 135 DSEIKGEF 142
>XP_006719618
          Length = 1394

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LKGVFT 9
           LKG+FT
Sbjct: 114 LKGIFT 119
>XP_006719617
          Length = 1401

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LKGVFT 9
           LKG+FT
Sbjct: 114 LKGIFT 119
>XP_006719616
          Length = 1405

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LKGVFT 9
           LKG+FT
Sbjct: 114 LKGIFT 119
>XP_006719615
          Length = 1429

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LKGVFT 9
           LKG+FT
Sbjct: 114 LKGIFT 119
>XP_006719614
          Length = 1440

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LKGVFT 9
           LKG+FT
Sbjct: 114 LKGIFT 119
>XP_005253652
          Length = 1440

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LKGVFT 9
           LKG+FT
Sbjct: 114 LKGIFT 119
>XP_005253651
          Length = 1440

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LKGVFT 9
           LKG+FT
Sbjct: 114 LKGIFT 119
>XP_005253650
          Length = 1440

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LKGVFT 9
           LKG+FT
Sbjct: 114 LKGIFT 119
>XP_005247591
          Length = 1036

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DSQLKGV 7
           DS+LKG+
Sbjct: 545 DSKLKGI 551
>XP_005247590
          Length = 1074

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DSQLKGV 7
           DS+LKG+
Sbjct: 545 DSKLKGI 551
>NP_001136254
          Length = 1481

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SQLKGV 7
           SQLKGV
Sbjct: 402 SQLKGV 407
>NP_116317
          Length = 108

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  LKGVFT 9
          +KGVFT
Sbjct: 32 MKGVFT 37
>NP_690864
          Length = 1125

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SQLKGV 7
           SQLKGV
Sbjct: 402 SQLKGV 407
>NP_060542
          Length = 2144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    QLKGVFT 9
            +LKG+FT
Sbjct: 1946 KLKGLFT 1952
>XP_005270794
          Length = 1125

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SQLKGV 7
           SQLKGV
Sbjct: 402 SQLKGV 407
>NP_115536
          Length = 1222

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   SQLKGVF 8
           SQ+KG F
Sbjct: 982 SQMKGAF 988
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.134    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 452,728
Number of extensions: 620
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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