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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              19     869
NP_009152                                                              17    4312
NP_001186582                                                           17    4312
NP_001186583                                                           17    4312
NP_001186591                                                           17    4312
NP_001186592                                                           17    4312
NP_001186590                                                           17    4312
NP_001186593                                                           17    4312
NP_003615                                                              15    9605
NP_015561                                                              15    9605
NP_015559                                                              15    9605
XP_005259721                                                           15    9605

>NP_004251
          Length = 1208

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DRVKLVDSM 9
           D VKLVDSM
Sbjct: 988 DMVKLVDSM 996



>NP_009152
          Length = 205

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  RVKLVDSM 9
          RVKL+D+M
Sbjct: 34 RVKLLDTM 41



>NP_001186582
          Length = 180

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  RVKLVDSM 9
          RVKL+D+M
Sbjct: 34 RVKLLDTM 41



>NP_001186583
          Length = 207

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  RVKLVDSM 9
          RVKL+D+M
Sbjct: 34 RVKLLDTM 41



>NP_001186591
          Length = 211

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  RVKLVDSM 9
          RVKL+D+M
Sbjct: 34 RVKLLDTM 41



>NP_001186592
          Length = 175

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  RVKLVDSM 9
          RVKL+D+M
Sbjct: 34 RVKLLDTM 41



>NP_001186590
          Length = 220

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  RVKLVDSM 9
          RVKL+D+M
Sbjct: 34 RVKLLDTM 41



>NP_001186593
          Length = 142

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  RVKLVDSM 9
          RVKL+D+M
Sbjct: 34 RVKLLDTM 41



>NP_003615
          Length = 562

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DRVKLVD 7
           DRVKL++
Sbjct: 263 DRVKLIN 269



>NP_015561
          Length = 567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DRVKLVD 7
           DRVKL++
Sbjct: 268 DRVKLIN 274



>NP_015559
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DRVKLVD 7
           DRVKL++
Sbjct: 200 DRVKLIN 206



>XP_005259721
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DRVKLVD 7
           DRVKL++
Sbjct: 195 DRVKLIN 201



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.136    0.346 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 394,274
Number of extensions: 595
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)