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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              20     509
NP_065769                                                              15    9605
NP_542165                                                              15    9605
NP_004631                                                              15    9605
NP_066015                                                              15    9605
XP_005258381                                                           15    9605
XP_006722580                                                           15    9605
XP_006722579                                                           15    9605
XP_005258380                                                           15    9605
XP_006721134                                                           15    9605
XP_005255507                                                           15    9605

>NP_000312
          Length = 928

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DQIMMFSMY 9
           DQIMM SMY
Sbjct: 701 DQIMMCSMY 709



>NP_065769
          Length = 1352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DQIMMFSMY 9
           DQI++  MY
Sbjct: 362 DQIVLTEMY 370



>NP_542165
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   QIMMFS 7
           Q+MMFS
Sbjct: 223 QVMMFS 228



>NP_004631
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   QIMMFS 7
           Q+MMFS
Sbjct: 223 QVMMFS 228



>NP_066015
          Length = 2579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DQIMMFSMY 9
           DQ+ M S+Y
Sbjct: 847 DQVGMVSLY 855



>XP_005258381
          Length = 2224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DQIMMFSMY 9
           DQ+ M S+Y
Sbjct: 492 DQVGMVSLY 500



>XP_006722580
          Length = 2532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DQIMMFSMY 9
           DQ+ M S+Y
Sbjct: 847 DQVGMVSLY 855



>XP_006722579
          Length = 2544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DQIMMFSMY 9
           DQ+ M S+Y
Sbjct: 847 DQVGMVSLY 855



>XP_005258380
          Length = 2578

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DQIMMFSMY 9
           DQ+ M S+Y
Sbjct: 847 DQVGMVSLY 855



>XP_006721134
          Length = 1089

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DQIMMFSMY 9
           DQI++  MY
Sbjct: 99  DQIVLTEMY 107



>XP_005255507
          Length = 1089

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DQIMMFSMY 9
           DQI++  MY
Sbjct: 99  DQIVLTEMY 107



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.347    0.141    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 259,385
Number of extensions: 415
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)