Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              19     869
NP_056147                                                              18    1935
NP_001273428                                                           16    5631
NP_001273425                                                           16    5631
NP_079325                                                              16    5631
NP_001273426                                                           16    5631
NP_001127835                                                           15    9605
NP_056392                                                              15    9605
NP_001138997                                                           15    9605
NP_001138995                                                           15    9605
NP_001138994                                                           15    9605
NP_001138993                                                           15    9605
NP_001138992                                                           15    9605
NP_000333                                                              15    9605
XP_005257650                                                           15    9605
XP_005257649                                                           15    9605
XP_006714532                                                           15    9605
NP_116168                                                              15    9605

>NP_004251
          Length = 1208

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DQALLSLLQ 9
           DQALL+LLQ
Sbjct: 685 DQALLTLLQ 693



>NP_056147
          Length = 361

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  DQALLSLLQ 9
          DQALL +LQ
Sbjct: 10 DQALLGILQ 18



>NP_001273428
          Length = 588

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DQALLSLLQ 9
           DQ  +SLLQ
Sbjct: 331 DQTFISLLQ 339



>NP_001273425
          Length = 641

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DQALLSLLQ 9
           DQ  +SLLQ
Sbjct: 384 DQTFISLLQ 392



>NP_079325
          Length = 641

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DQALLSLLQ 9
           DQ  +SLLQ
Sbjct: 384 DQTFISLLQ 392



>NP_001273426
          Length = 707

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DQALLSLLQ 9
           DQ  +SLLQ
Sbjct: 384 DQTFISLLQ 392



>NP_001127835
          Length = 1227

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   DQALLSL 7
           DQALLS+
Sbjct: 385 DQALLSV 391



>NP_056392
          Length = 980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   QALLSLLQ 9
           Q LLSLLQ
Sbjct: 346 QDLLSLLQ 353



>NP_001138997
          Length = 983

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   QALLSLLQ 9
           Q LLSLLQ
Sbjct: 349 QDLLSLLQ 356



>NP_001138995
          Length = 972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   QALLSLLQ 9
           Q LLSLLQ
Sbjct: 338 QDLLSLLQ 345



>NP_001138994
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   QALLSLLQ 9
           Q LLSLLQ
Sbjct: 317 QDLLSLLQ 324



>NP_001138993
          Length = 980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   QALLSLLQ 9
           Q LLSLLQ
Sbjct: 346 QDLLSLLQ 353



>NP_001138992
          Length = 980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   QALLSLLQ 9
           Q LLSLLQ
Sbjct: 346 QDLLSLLQ 353



>NP_000333
          Length = 911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   DQALLSLL 8
           +QALLSL+
Sbjct: 329 EQALLSLV 336



>XP_005257650
          Length = 846

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   DQALLSLL 8
           +QALLSL+
Sbjct: 264 EQALLSLV 271



>XP_005257649
          Length = 911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   DQALLSLL 8
           +QALLSL+
Sbjct: 329 EQALLSLV 336



>XP_006714532
          Length = 980

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   QALLSLLQ 9
           Q LLSLLQ
Sbjct: 346 QDLLSLLQ 353



>NP_116168
          Length = 743

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   DQALLSLLQ 9
           D ALL LLQ
Sbjct: 635 DGALLCLLQ 643



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.135    0.316 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 305,334
Number of extensions: 552
Number of successful extensions: 18
Number of sequences better than 10000.0: 18
Number of HSP's gapped: 18
Number of HSP's successfully gapped: 18
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)