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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_064450                                                              19     665
XP_005273257                                                           19     665
NP_829884                                                              17    4312
NP_829883                                                              17    4312
NP_001122107                                                           16    5631
NP_001122105                                                           16    5631
NP_056487                                                              16    5631
NP_065801                                                              16    7355
NP_001138830                                                           15    9605
NP_001138829                                                           15    9605
NP_056292                                                              15    9605
NP_008949                                                              15    9605
NP_001034669                                                           15    9605
NP_079467                                                              15    9605
XP_006720530                                                           15    9605
XP_006720529                                                           15    9605
XP_006720528                                                           15    9605
XP_006717006                                                           15    9605
XP_006717005                                                           15    9605
XP_006717004                                                           15    9605
XP_006717003                                                           15    9605
XP_005251736                                                           15    9605
XP_006714371                                                           15    9605
XP_006714370                                                           15    9605
XP_006714369                                                           15    9605
XP_005245556                                                           15    9605

>NP_064450
          Length = 1722

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DQAAEENSL 9
            DQ AEENSL
Sbjct: 1623 DQEAEENSL 1631



>XP_005273257
          Length = 1726

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DQAAEENSL 9
            DQ AEENSL
Sbjct: 1627 DQEAEENSL 1635



>NP_829884
          Length = 1116

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    DQAAEENSL 9
            DQAA EN L
Sbjct: 1056 DQAAWENEL 1064



>NP_829883
          Length = 1088

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1    DQAAEENSL 9
            DQAA EN L
Sbjct: 1028 DQAAWENEL 1036



>NP_001122107
          Length = 1639

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   DQAAEENSL 9
           D+A EE+SL
Sbjct: 923 DEALEEDSL 931



>NP_001122105
          Length = 1639

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   DQAAEENSL 9
           D+A EE+SL
Sbjct: 923 DEALEEDSL 931



>NP_056487
          Length = 1639

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   DQAAEENSL 9
           D+A EE+SL
Sbjct: 923 DEALEEDSL 931



>NP_065801
          Length = 1204

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DQAAEEN 7
           D+AA+EN
Sbjct: 944 DEAADEN 950



>NP_001138830
          Length = 1273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DQAAEENS 8
           +QA EEN+
Sbjct: 762 EQATEENA 769



>NP_001138829
          Length = 1273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DQAAEENS 8
           +QA EEN+
Sbjct: 762 EQATEENA 769



>NP_056292
          Length = 1273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DQAAEENS 8
           +QA EEN+
Sbjct: 762 EQATEENA 769



>NP_008949
          Length = 2325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AAEENS 8
            AAEENS
Sbjct: 1812 AAEENS 1817



>NP_001034669
          Length = 647

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DQAAEENSL 9
          D +A+ENS+
Sbjct: 61 DTSADENSV 69



>NP_079467
          Length = 932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QAAEENSL 9
           +++EENSL
Sbjct: 833 ESSEENSL 840



>XP_006720530
          Length = 1273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DQAAEENS 8
           +QA EEN+
Sbjct: 762 EQATEENA 769



>XP_006720529
          Length = 1273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DQAAEENS 8
           +QA EEN+
Sbjct: 762 EQATEENA 769



>XP_006720528
          Length = 1273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DQAAEENS 8
           +QA EEN+
Sbjct: 762 EQATEENA 769



>XP_006717006
          Length = 1760

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AAEENS 8
            AAEENS
Sbjct: 1232 AAEENS 1237



>XP_006717005
          Length = 1788

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AAEENS 8
            AAEENS
Sbjct: 1260 AAEENS 1265



>XP_006717004
          Length = 2240

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AAEENS 8
            AAEENS
Sbjct: 1812 AAEENS 1817



>XP_006717003
          Length = 2278

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AAEENS 8
            AAEENS
Sbjct: 1812 AAEENS 1817



>XP_005251736
          Length = 2325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    AAEENS 8
            AAEENS
Sbjct: 1812 AAEENS 1817



>XP_006714371
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DQAAEENSL 9
          D +A+ENS+
Sbjct: 61 DTSADENSV 69



>XP_006714370
          Length = 622

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DQAAEENSL 9
          D +A+ENS+
Sbjct: 61 DTSADENSV 69



>XP_006714369
          Length = 646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DQAAEENSL 9
          D +A+ENS+
Sbjct: 61 DTSADENSV 69



>XP_005245556
          Length = 933

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QAAEENSL 9
           +++EENSL
Sbjct: 834 ESSEENSL 841



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.282    0.103    0.235 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 499,184
Number of extensions: 759
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.7 bits)
S2: 28 (15.4 bits)