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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_036462                                                              18    1935
NP_001243397                                                           18    1935
XP_005269721                                                           18    1935
NP_443100                                                              16    5631
NP_001193594                                                           16    5631
XP_006711694                                                           16    5631
XP_005245671                                                           16    5631
NP_037444                                                              15    9605
NP_001093646                                                           15    9605
XP_005259931                                                           15    9605
XP_005259930                                                           15    9605

>NP_036462
          Length = 2073

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DLSSRLAVT 9
           D SSRLAVT
Sbjct: 394 DSSSRLAVT 402



>NP_001243397
          Length = 1890

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DLSSRLAVT 9
           D SSRLAVT
Sbjct: 394 DSSSRLAVT 402



>XP_005269721
          Length = 2073

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DLSSRLAVT 9
           D SSRLAVT
Sbjct: 394 DSSSRLAVT 402



>NP_443100
          Length = 613

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LSSRLAVT 9
           LSS+LAVT
Sbjct: 301 LSSQLAVT 308



>NP_001193594
          Length = 613

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LSSRLAVT 9
           LSS+LAVT
Sbjct: 301 LSSQLAVT 308



>XP_006711694
          Length = 596

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LSSRLAVT 9
           LSS+LAVT
Sbjct: 284 LSSQLAVT 291



>XP_005245671
          Length = 613

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   LSSRLAVT 9
           LSS+LAVT
Sbjct: 301 LSSQLAVT 308



>NP_037444
          Length = 719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  DLSSRLAV 8
          DLSS LAV
Sbjct: 30 DLSSGLAV 37



>NP_001093646
          Length = 717

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  DLSSRLAV 8
          DLSS LAV
Sbjct: 30 DLSSGLAV 37



>XP_005259931
          Length = 716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  DLSSRLAV 8
          DLSS LAV
Sbjct: 30 DLSSGLAV 37



>XP_005259930
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  DLSSRLAV 8
          DLSS LAV
Sbjct: 30 DLSSGLAV 37



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.122    0.286 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 246,632
Number of extensions: 241
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)