Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001895                                                              19     869
NP_001091680                                                           19     869
NP_001091679                                                           19     869
XP_006713048                                                           19     869
XP_005264943                                                           19     869
NP_001035232                                                           16    5631
NP_066287                                                              16    5631
NP_001035233                                                           16    5631
XP_005246813                                                           16    5631
XP_005246812                                                           16    5631
XP_005246811                                                           16    5631
XP_005246810                                                           16    5631
XP_005246808                                                           16    5631
XP_005246807                                                           16    5631
NP_113656                                                              16    7355
NP_001017440                                                           16    7355
XP_006716209                                                           16    7355
>NP_001895
          Length = 781

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  DLMELGMAM 9
          DLMEL MAM
Sbjct: 6  DLMELDMAM 14
>NP_001091680
          Length = 781

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  DLMELGMAM 9
          DLMEL MAM
Sbjct: 6  DLMELDMAM 14
>NP_001091679
          Length = 781

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  DLMELGMAM 9
          DLMEL MAM
Sbjct: 6  DLMELDMAM 14
>XP_006713048
          Length = 720

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  DLMELGMAM 9
          DLMEL MAM
Sbjct: 6  DLMELDMAM 14
>XP_005264943
          Length = 781

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  DLMELGMAM 9
          DLMEL MAM
Sbjct: 6  DLMELDMAM 14
>NP_001035232
          Length = 2005

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LMELGMA 8
           LMELG+A
Sbjct: 835 LMELGLA 841
>NP_066287
          Length = 2005

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LMELGMA 8
           LMELG+A
Sbjct: 835 LMELGLA 841
>NP_001035233
          Length = 2005

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LMELGMA 8
           LMELG+A
Sbjct: 835 LMELGLA 841
>XP_005246813
          Length = 1271

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LMELGMA 8
           LMELG+A
Sbjct: 101 LMELGLA 107
>XP_005246812
          Length = 1754

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LMELGMA 8
           LMELG+A
Sbjct: 584 LMELGLA 590
>XP_005246811
          Length = 1995

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LMELGMA 8
           LMELG+A
Sbjct: 825 LMELGLA 831
>XP_005246810
          Length = 2005

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LMELGMA 8
           LMELG+A
Sbjct: 835 LMELGLA 841
>XP_005246808
          Length = 2005

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LMELGMA 8
           LMELG+A
Sbjct: 835 LMELGLA 841
>XP_005246807
          Length = 2005

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LMELGMA 8
           LMELG+A
Sbjct: 835 LMELGLA 841
>NP_113656
          Length = 261

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ELGMAM 9
           ELGMAM
Sbjct: 102 ELGMAM 107
>NP_001017440
          Length = 219

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ELGMAM 9
          ELGMAM
Sbjct: 60 ELGMAM 65
>XP_006716209
          Length = 134

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  ELGMAM 9
          ELGMAM
Sbjct: 60 ELGMAM 65
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.139    0.368 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 402,627
Number of extensions: 484
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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