Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     509
XP_005262424                                                           20     509
NP_006794                                                              17    2528
NP_001127768                                                           17    2528
XP_006716339                                                           17    2528
NP_073617                                                              16    5631
NP_001248755                                                           16    5631
NP_003929                                                              16    5631
NP_001245240                                                           16    5631
NP_689757                                                              16    5631
XP_006722995                                                           16    5631
XP_005248007                                                           16    5631
XP_006713925                                                           16    5631
NP_777582                                                              16    7355
XP_006726821                                                           16    7355
XP_006726820                                                           16    7355
XP_006726819                                                           16    7355
XP_006726818                                                           16    7355
XP_006726817                                                           16    7355
XP_006726816                                                           16    7355
XP_006726815                                                           16    7355
XP_006726814                                                           16    7355
XP_006726813                                                           16    7355
XP_006726812                                                           16    7355
XP_006726811                                                           16    7355
XP_006726810                                                           16    7355
XP_006722325                                                           16    7355
XP_006716590                                                           16    7355
XP_006716589                                                           16    7355
XP_005269407                                                           16    7355
XP_006710099                                                           16    7355
NP_037506                                                              15    9605
NP_001155901                                                           15    9605
NP_001243121                                                           15    9605
NP_057070                                                              15    9605
XP_006722004                                                           15    9605
>NP_722576
          Length = 3080

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    DLKGTMSL 8
            DLKGTMSL
Sbjct: 2938 DLKGTMSL 2945
>XP_005262424
          Length = 2875

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    DLKGTMSL 8
            DLKGTMSL
Sbjct: 2733 DLKGTMSL 2740
>NP_006794
          Length = 418

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   LKGTMSL 8
           LKGTMSL
Sbjct: 354 LKGTMSL 360
>NP_001127768
          Length = 418

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   LKGTMSL 8
           LKGTMSL
Sbjct: 354 LKGTMSL 360
>XP_006716339
          Length = 418

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   LKGTMSL 8
           LKGTMSL
Sbjct: 354 LKGTMSL 360
>NP_073617
          Length = 391

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DLKGTMSLI 9
          +L GT+SL+
Sbjct: 52 ELSGTLSLV 60
>NP_001248755
          Length = 1215

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LKGTMSLI 9
            LKGT+S I
Sbjct: 1079 LKGTLSFI 1086
>NP_003929
          Length = 1153

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LKGTMSLI 9
            LKGT+S I
Sbjct: 1017 LKGTLSFI 1024
>NP_001245240
          Length = 524

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LKGTMSLI 9
           +KG MSLI
Sbjct: 397 MKGNMSLI 404
>NP_689757
          Length = 757

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DLKGTMSLI 9
           D K T+SLI
Sbjct: 289 DFKSTLSLI 297
>XP_006722995
          Length = 1203

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LKGTMSLI 9
            LKGT+S I
Sbjct: 1067 LKGTLSFI 1074
>XP_005248007
          Length = 516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DLKGTMSLI 9
          D K T+SLI
Sbjct: 48 DFKSTLSLI 56
>XP_006713925
          Length = 685

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DLKGTMSLI 9
           D K T+SLI
Sbjct: 289 DFKSTLSLI 297
>NP_777582
          Length = 580

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DLKGTMS 7
           DLKGT++
Sbjct: 267 DLKGTLA 273
>XP_006726821
          Length = 408

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 157 KGTMSL 162
>XP_006726820
          Length = 415

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 285 KGTMSL 290
>XP_006726819
          Length = 468

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 285 KGTMSL 290
>XP_006726818
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 263 KGTMSL 268

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 369 KGTMSL 374
>XP_006726817
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 157 KGTMSL 162

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 369 KGTMSL 374
>XP_006726816
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 157 KGTMSL 162
>XP_006726815
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 263 KGTMSL 268

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 369 KGTMSL 374
>XP_006726814
          Length = 483

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 285 KGTMSL 290
>XP_006726813
          Length = 483

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 285 KGTMSL 290

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 391 KGTMSL 396
>XP_006726812
          Length = 499

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 285 KGTMSL 290

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 391 KGTMSL 396
>XP_006726811
          Length = 499

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 157 KGTMSL 162
>XP_006726810
          Length = 499

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 157 KGTMSL 162
>XP_006722325
          Length = 408

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 157 KGTMSL 162
>XP_006716590
          Length = 473

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DLKGTMS 7
           DLKGT++
Sbjct: 160 DLKGTLA 166
>XP_006716589
          Length = 568

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DLKGTMS 7
           DLKGT++
Sbjct: 267 DLKGTLA 273
>XP_005269407
          Length = 144

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  LKGTMS 7
          LKGTMS
Sbjct: 31 LKGTMS 36
>XP_006710099
          Length = 441

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KGTMSL 8
           KGTMSL
Sbjct: 210 KGTMSL 215
>NP_037506
          Length = 868

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DLKGTMSLI 9
           D  GT+SLI
Sbjct: 182 DTVGTLSLI 190
>NP_001155901
          Length = 873

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DLKGTMSLI 9
           D  GT+SLI
Sbjct: 182 DTVGTLSLI 190
>NP_001243121
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DLKGTMSLI 9
           D  GT+SLI
Sbjct: 182 DTVGTLSLI 190
>NP_057070
          Length = 442

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  DLKGTM 6
          DLKGT+
Sbjct: 61 DLKGTL 66
>XP_006722004
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  DLKGTM 6
          DLKGT+
Sbjct: 19 DLKGTL 24
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.319    0.138    0.358 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 422,563
Number of extensions: 481
Number of successful extensions: 41
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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