Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              20     390
NP_055717                                                              17    3301
XP_005260386                                                           17    3301
NP_443171                                                              17    4312
NP_112572                                                              17    4312
XP_006726167                                                           17    4312
XP_006726072                                                           17    4312
XP_006725979                                                           17    4312
XP_006725887                                                           17    4312
XP_006725770                                                           17    4312
XP_006725556                                                           17    4312
XP_006715213                                                           17    4312
XP_006711210                                                           17    4312
XP_006711209                                                           17    4312
XP_005244929                                                           17    4312
XP_006711208                                                           17    4312
NP_001073991                                                           16    5631
NP_065836                                                              16    5631
NP_001158192                                                           16    5631
NP_705838                                                              16    5631
NP_612429                                                              16    5631
NP_573420                                                              16    5631
NP_573419                                                              16    5631
NP_573418                                                              16    5631
NP_006042                                                              16    5631
NP_071371                                                              16    5631
NP_060667                                                              16    5631
NP_955458                                                              16    5631
XP_005260506                                                           16    5631
XP_006723884                                                           16    5631
XP_005267356                                                           16    5631
XP_006714033                                                           16    5631
XP_005248236                                                           16    5631
XP_005248234                                                           16    5631
NP_542194                                                              16    7355
NP_689488                                                              16    7355
NP_000290                                                              15    9605
NP_001005337                                                           15    9605
NP_055778                                                              15    9605
NP_001093592                                                           15    9605
XP_005259577                                                           15    9605
XP_005259576                                                           15    9605
>NP_115803
          Length = 1156

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DLGRQSQRS 9
           DLG+QSQRS
Sbjct: 343 DLGQQSQRS 351
>NP_055717
          Length = 989

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LGRQSQRS 9
           LGRQ++RS
Sbjct: 230 LGRQAERS 237
>XP_005260386
          Length = 992

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LGRQSQRS 9
           LGRQ++RS
Sbjct: 230 LGRQAERS 237
>NP_443171
          Length = 734

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LGRQSQRS 9
           LGR++QRS
Sbjct: 331 LGRKTQRS 338
>NP_112572
          Length = 515

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LGRQSQRS 9
           LGR++QRS
Sbjct: 332 LGRKTQRS 339
>XP_006726167
          Length = 156

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2 LGRQSQRS 9
         +GR SQRS
Sbjct: 1 MGRSSQRS 8
>XP_006726072
          Length = 156

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2 LGRQSQRS 9
         +GR SQRS
Sbjct: 1 MGRSSQRS 8
>XP_006725979
          Length = 156

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2 LGRQSQRS 9
         +GR SQRS
Sbjct: 1 MGRSSQRS 8
>XP_006725887
          Length = 156

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2 LGRQSQRS 9
         +GR SQRS
Sbjct: 1 MGRSSQRS 8
>XP_006725770
          Length = 156

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2 LGRQSQRS 9
         +GR SQRS
Sbjct: 1 MGRSSQRS 8
>XP_006725556
          Length = 156

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2 LGRQSQRS 9
         +GR SQRS
Sbjct: 1 MGRSSQRS 8
>XP_006715213
          Length = 156

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2 LGRQSQRS 9
         +GR SQRS
Sbjct: 1 MGRSSQRS 8
>XP_006711210
          Length = 485

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  LGRQSQRS 9
          LGR++QRS
Sbjct: 82 LGRKTQRS 89
>XP_006711209
          Length = 613

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LGRQSQRS 9
           LGR++QRS
Sbjct: 331 LGRKTQRS 338
>XP_005244929
          Length = 647

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LGRQSQRS 9
           LGR++QRS
Sbjct: 244 LGRKTQRS 251
>XP_006711208
          Length = 734

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LGRQSQRS 9
           LGR++QRS
Sbjct: 331 LGRKTQRS 338
>NP_001073991
          Length = 1620

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DLGRQSQRS 9
          D+GRQ++ S
Sbjct: 23 DMGRQNKNS 31
>NP_065836
          Length = 122

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DLGRQSQRS 9
          D+GRQ++ S
Sbjct: 23 DMGRQNKNS 31
>NP_001158192
          Length = 111

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DLGRQSQRS 9
          D+GRQ++ S
Sbjct: 23 DMGRQNKNS 31
>NP_705838
          Length = 4069

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    DLGRQS 6
            DLGRQS
Sbjct: 1644 DLGRQS 1649
>NP_612429
          Length = 5795

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   GRQSQR 8
           GRQSQR
Sbjct: 251 GRQSQR 256
>NP_573420
          Length = 484

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DLGRQS 6
           DLGRQS
Sbjct: 377 DLGRQS 382
>NP_573419
          Length = 486

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DLGRQS 6
           DLGRQS
Sbjct: 379 DLGRQS 384
>NP_573418
          Length = 491

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DLGRQS 6
           DLGRQS
Sbjct: 384 DLGRQS 389
>NP_006042
          Length = 493

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DLGRQS 6
           DLGRQS
Sbjct: 386 DLGRQS 391
>NP_071371
          Length = 627

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   DLGRQSQR 8
           D GRQS R
Sbjct: 353 DTGRQSSR 360
>NP_060667
          Length = 681

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   DLGRQSQR 8
           D GRQS R
Sbjct: 407 DTGRQSSR 414
>NP_955458
          Length = 679

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   DLGRQSQR 8
           D GRQS R
Sbjct: 405 DTGRQSSR 412
>XP_005260506
          Length = 523

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   DLGRQSQR 8
           D GRQS R
Sbjct: 249 DTGRQSSR 256
>XP_006723884
          Length = 625

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   DLGRQSQR 8
           D GRQS R
Sbjct: 351 DTGRQSSR 358
>XP_005267356
          Length = 5695

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   GRQSQR 8
           GRQSQR
Sbjct: 151 GRQSQR 156
>XP_006714033
          Length = 111

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DLGRQSQRS 9
          D+GRQ++ S
Sbjct: 23 DMGRQNKNS 31
>XP_005248236
          Length = 1262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DLGRQSQRS 9
          D+GRQ++ S
Sbjct: 23 DMGRQNKNS 31
>XP_005248234
          Length = 1620

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DLGRQSQRS 9
          D+GRQ++ S
Sbjct: 23 DMGRQNKNS 31
>NP_542194
          Length = 1661

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    GRQSQRS 9
            GRQ QRS
Sbjct: 1235 GRQMQRS 1241
>NP_689488
          Length = 665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  LGRQSQRS 9
          LGR+ QRS
Sbjct: 34 LGRELQRS 41
>NP_000290
          Length = 747

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DLGRQSQRS 9
           D GRQ+ R+
Sbjct: 438 DAGRQTMRN 446
>NP_001005337
          Length = 726

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DLGRQSQRS 9
           D GRQ+ R+
Sbjct: 417 DAGRQTMRN 425
>NP_055778
          Length = 1366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    DLGRQSQ 7
            ++GRQSQ
Sbjct: 1097 NIGRQSQ 1103
>NP_001093592
          Length = 1309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    DLGRQSQ 7
            ++GRQSQ
Sbjct: 1040 NIGRQSQ 1046
>XP_005259577
          Length = 1366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    DLGRQSQ 7
            ++GRQSQ
Sbjct: 1097 NIGRQSQ 1103
>XP_005259576
          Length = 1366

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    DLGRQSQ 7
            ++GRQSQ
Sbjct: 1097 NIGRQSQ 1103
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.123    0.316 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 446,253
Number of extensions: 442
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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