Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     869
NP_001075109                                                           19     869
NP_892018                                                              17    4312
XP_005259608                                                           17    4312
NP_037518                                                              16    5631
NP_998816                                                              16    5631
NP_004955                                                              15    9605
NP_004963                                                              15    9605
NP_001518                                                              15    9605
NP_001122060                                                           15    9605
NP_056949                                                              15    9605
NP_001139508                                                           15    9605
NP_001139509                                                           15    9605
NP_002802                                                              15    9605
XP_005258390                                                           15    9605
XP_005258389                                                           15    9605
XP_005258386                                                           15    9605
XP_006722589                                                           15    9605
XP_005251512                                                           15    9605

>NP_008835
          Length = 4128

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    DLAVLELMR 9
            DLAVLELM+
Sbjct: 1576 DLAVLELMQ 1584



>NP_001075109
          Length = 4097

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    DLAVLELMR 9
            DLAVLELM+
Sbjct: 1576 DLAVLELMQ 1584



>NP_892018
          Length = 1059

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   DLAVLEL 7
           DLAVLEL
Sbjct: 592 DLAVLEL 598




 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   DLAVLEL 7
           D+AVLEL
Sbjct: 292 DVAVLEL 298



>XP_005259608
          Length = 1093

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   DLAVLEL 7
           DLAVLEL
Sbjct: 626 DLAVLEL 632




 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   DLAVLEL 7
           D+AVLEL
Sbjct: 326 DVAVLEL 332



>NP_037518
          Length = 477

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   DLAVLELMR 9
           DLAV EL++
Sbjct: 361 DLAVYELLK 369



>NP_998816
          Length = 458

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   DLAVLELMR 9
           DLAV EL++
Sbjct: 342 DLAVYELLK 350



>NP_004955
          Length = 482

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LAVLELMR 9
           LA+LEL++
Sbjct: 158 LAILELLK 165



>NP_004963
          Length = 1132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LAVLELMR 9
           +AVL++MR
Sbjct: 181 MAVLDMMR 188



>NP_001518
          Length = 488

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LAVLELMR 9
           LA+LEL++
Sbjct: 159 LAILELLK 166



>NP_001122060
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DLAVLELMR 9
           DL+ LEL++
Sbjct: 272 DLSALELLK 280



>NP_056949
          Length = 389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DLAVLELMR 9
           DL+ LEL++
Sbjct: 216 DLSALELLK 224



>NP_001139508
          Length = 389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DLAVLELMR 9
           DL+ LEL++
Sbjct: 216 DLSALELLK 224



>NP_001139509
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DLAVLELMR 9
           DL+ LEL++
Sbjct: 272 DLSALELLK 280



>NP_002802
          Length = 324

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DLAVLELMR 9
           D+A+ ELM+
Sbjct: 105 DIAINELMK 113



>XP_005258390
          Length = 257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DLAVLELMR 9
          DL+ LEL++
Sbjct: 84 DLSALELLK 92



>XP_005258389
          Length = 257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DLAVLELMR 9
          DL+ LEL++
Sbjct: 84 DLSALELLK 92



>XP_005258386
          Length = 402

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DLAVLELMR 9
           DL+ LEL++
Sbjct: 272 DLSALELLK 280



>XP_006722589
          Length = 424

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DLAVLELMR 9
           DL+ LEL++
Sbjct: 251 DLSALELLK 259



>XP_005251512
          Length = 1132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LAVLELMR 9
           +AVL++MR
Sbjct: 181 MAVLDMMR 188



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.147    0.366 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 334,182
Number of extensions: 394
Number of successful extensions: 21
Number of sequences better than 10000.0: 19
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)