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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001268421                                                           19     869
NP_000170                                                              19     869
NP_001268422                                                           19     869
NP_001268423                                                           19     869
XP_005264328                                                           19     869
XP_006720393                                                           18    1935
XP_006720392                                                           18    1935
XP_006720391                                                           18    1935
NP_056327                                                              17    4312
NP_001753                                                              17    4312
NP_001009186                                                           17    4312
XP_006713163                                                           17    4312
NP_001095940                                                           16    5631
NP_000663                                                              16    5631
XP_005266602                                                           16    7355
NP_001277002                                                           15    9605
NP_001258709                                                           15    9605
NP_001129628                                                           15    9605
NP_055629                                                              15    9605
NP_001258708                                                           15    9605
NP_001258710                                                           15    9605
NP_067547                                                              15    9605
NP_006575                                                              15    9605
NP_001180459                                                           15    9605
NP_001180458                                                           15    9605
NP_067037                                                              15    9605
NP_001277001                                                           15    9605
NP_055725                                                              15    9605
XP_006721705                                                           15    9605
XP_006721704                                                           15    9605
XP_005249317                                                           15    9605
XP_006713494                                                           15    9605
XP_005265676                                                           15    9605
XP_005265675                                                           15    9605

>NP_001268421
          Length = 1230

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DKISKVVEL 9
           DKIS+VVEL
Sbjct: 673 DKISEVVEL 681



>NP_000170
          Length = 1360

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DKISKVVEL 9
           DKIS+VVEL
Sbjct: 803 DKISEVVEL 811



>NP_001268422
          Length = 1058

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DKISKVVEL 9
           DKIS+VVEL
Sbjct: 501 DKISEVVEL 509



>NP_001268423
          Length = 1058

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DKISKVVEL 9
           DKIS+VVEL
Sbjct: 501 DKISEVVEL 509



>XP_005264328
          Length = 1261

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DKISKVVEL 9
           DKIS+VVEL
Sbjct: 704 DKISEVVEL 712



>XP_006720393
          Length = 465

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KISKVVEL 9
           K+SKVVEL
Sbjct: 457 KVSKVVEL 464



>XP_006720392
          Length = 465

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KISKVVEL 9
           K+SKVVEL
Sbjct: 457 KVSKVVEL 464



>XP_006720391
          Length = 465

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   KISKVVEL 9
           K+SKVVEL
Sbjct: 457 KVSKVVEL 464



>NP_056327
          Length = 4265

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    DKISKVVEL 9
            +K+S++VEL
Sbjct: 2094 EKLSRIVEL 2102



>NP_001753
          Length = 531

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   DKISKVVEL 9
           D++ K++EL
Sbjct: 269 DRVKKIIEL 277



>NP_001009186
          Length = 486

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   DKISKVVEL 9
           D++ K++EL
Sbjct: 224 DRVKKIIEL 232



>XP_006713163
          Length = 4265

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    DKISKVVEL 9
            +K+S++VEL
Sbjct: 2094 EKLSRIVEL 2102



>NP_001095940
          Length = 375

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   DKISKVVEL 9
           DKI++ VEL
Sbjct: 354 DKINEAVEL 362



>NP_000663
          Length = 368

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   DKISKVVEL 9
           DKI++ VEL
Sbjct: 354 DKINEAVEL 362



>XP_005266602
          Length = 922

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  DKISKVVEL 9
          D+I KVV+L
Sbjct: 11 DEIRKVVDL 19



>NP_001277002
          Length = 383

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 1  DKISKVVE 8
          D+++K++E
Sbjct: 71 DRVAKIIE 78



>NP_001258709
          Length = 351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   DKISKVVE 8
           DK++++VE
Sbjct: 310 DKVNEIVE 317



>NP_001129628
          Length = 953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  KISKVVE 8
          K+SK+VE
Sbjct: 50 KLSKIVE 56



>NP_055629
          Length = 389

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   DKISKVVE 8
           DK++++VE
Sbjct: 348 DKVNEIVE 355



>NP_001258708
          Length = 442

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   DKISKVVE 8
           DK++++VE
Sbjct: 401 DKVNEIVE 408



>NP_001258710
          Length = 350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   DKISKVVE 8
           DK++++VE
Sbjct: 309 DKVNEIVE 316



>NP_067547
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 1   DKISKVVE 8
           D+++K++E
Sbjct: 165 DRVAKIIE 172



>NP_006575
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/9 (33%), Positives = 8/9 (88%)

Query: 1   DKISKVVEL 9
           D++ K+++L
Sbjct: 269 DRVQKIIDL 277



>NP_001180459
          Length = 485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/9 (33%), Positives = 8/9 (88%)

Query: 1   DKISKVVEL 9
           D++ K+++L
Sbjct: 224 DRVQKIIDL 232



>NP_001180458
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/9 (33%), Positives = 8/9 (88%)

Query: 1   DKISKVVEL 9
           D++ K+++L
Sbjct: 232 DRVQKIIDL 240



>NP_067037
          Length = 329

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DKISKV 6
           DKISKV
Sbjct: 259 DKISKV 264



>NP_001277001
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 1   DKISKVVE 8
           D+++K++E
Sbjct: 165 DRVAKIIE 172



>NP_055725
          Length = 953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  KISKVVE 8
          K+SK+VE
Sbjct: 50 KLSKIVE 56



>XP_006721705
          Length = 464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/9 (33%), Positives = 8/9 (88%)

Query: 1   DKISKVVEL 9
           D++ K+++L
Sbjct: 203 DRVQKIIDL 211



>XP_006721704
          Length = 516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/9 (33%), Positives = 8/9 (88%)

Query: 1   DKISKVVEL 9
           D++ K+++L
Sbjct: 269 DRVQKIIDL 277



>XP_005249317
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 1   DKISKVVE 8
           D+++K++E
Sbjct: 165 DRVAKIIE 172



>XP_006713494
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   DKISKVVE 8
           DK++++VE
Sbjct: 299 DKVNEIVE 306



>XP_005265676
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   DKISKVVE 8
           DK++++VE
Sbjct: 299 DKVNEIVE 306



>XP_005265675
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   DKISKVVE 8
           DK++++VE
Sbjct: 369 DKVNEIVE 376



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.133    0.308 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 268,399
Number of extensions: 395
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)