Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_036557                                                              18    1135
XP_005252609                                                           18    1135
NP_620686                                                              16    7355
NP_001765                                                              16    7355
NP_689801                                                              16    7355
NP_006527                                                              16    7355
XP_005259492                                                           16    7355
XP_005271476                                                           16    7355
XP_006718836                                                           16    7355
NP_005996                                                              15    9605
NP_001139325                                                           15    9605
NP_001193865                                                           15    9605
NP_001193895                                                           15    9605
NP_001193858                                                           15    9605
NP_001193869                                                           15    9605
NP_001193861                                                           15    9605
NP_001193883                                                           15    9605
NP_001193863                                                           15    9605
NP_001127                                                              15    9605
NP_751947                                                              15    9605
NP_003391                                                              15    9605
NP_001004339                                                           15    9605
NP_057445                                                              15    9605
XP_006712160                                                           15    9605
XP_006712159                                                           15    9605
XP_006712158                                                           15    9605
XP_005264603                                                           15    9605
XP_005264601                                                           15    9605
XP_006712157                                                           15    9605
XP_006711427                                                           15    9605
XP_005277457                                                           15    9605
XP_006711426                                                           15    9605
XP_006710709                                                           15    9605
XP_001718723                                                           15    9605
NP_940857                                                              15    9605
>NP_036557
          Length = 277

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  DITGLFTMS 9
          D+ GLFT+S
Sbjct: 32 DVNGLFTLS 40
>XP_005252609
          Length = 277

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  DITGLFTMS 9
          D+ GLFT+S
Sbjct: 32 DVNGLFTLS 40
>NP_620686
          Length = 950

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ITGLFT 7
           +TGLFT
Sbjct: 685 VTGLFT 690
>NP_001765
          Length = 281

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  ITGLFTM 8
          I+G+FTM
Sbjct: 63 ISGIFTM 69
>NP_689801
          Length = 836

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ITGLFT 7
           +TGLFT
Sbjct: 98  VTGLFT 103
>NP_006527
          Length = 943

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   DITGLF 6
           DITG+F
Sbjct: 199 DITGIF 204
>XP_005259492
          Length = 365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  ITGLFTM 8
          I+G+FTM
Sbjct: 63 ISGIFTM 69
>XP_005271476
          Length = 619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ITGLFT 7
           +TGLFT
Sbjct: 354 VTGLFT 359
>XP_006718836
          Length = 642

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ITGLFT 7
           +TGLFT
Sbjct: 377 VTGLFT 382
>NP_005996
          Length = 890

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ITGLFTMS 9
           ITG FT++
Sbjct: 435 ITGFFTVT 442
>NP_001139325
          Length = 890

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ITGLFTMS 9
           ITG FT++
Sbjct: 435 ITGFFTVT 442
>NP_001193865
          Length = 355

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TGLFTM 8
           TGLFT+
Sbjct: 183 TGLFTL 188
>NP_001193895
          Length = 347

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TGLFTM 8
           TGLFT+
Sbjct: 183 TGLFTL 188
>NP_001193858
          Length = 420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TGLFTM 8
           TGLFT+
Sbjct: 199 TGLFTL 204
>NP_001193869
          Length = 347

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TGLFTM 8
           TGLFT+
Sbjct: 183 TGLFTL 188
>NP_001193861
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TGLFTM 8
           TGLFT+
Sbjct: 169 TGLFTL 174
>NP_001193883
          Length = 325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TGLFTM 8
           TGLFT+
Sbjct: 183 TGLFTL 188
>NP_001193863
          Length = 363

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TGLFTM 8
           TGLFT+
Sbjct: 199 TGLFTL 204
>NP_001127
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TGLFTM 8
           TGLFT+
Sbjct: 183 TGLFTL 188
>NP_751947
          Length = 342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TGLFTM 8
           TGLFT+
Sbjct: 169 TGLFTL 174
>NP_003391
          Length = 1071

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    ITGLFTMS 9
            +TGLF+++
Sbjct: 998  VTGLFSLN 1005
>NP_001004339
          Length = 759

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DITGLFTM 8
           D+T LFT+
Sbjct: 530 DVTFLFTL 537
>NP_057445
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2 ITGLFTM 8
         ITG+F+M
Sbjct: 2 ITGVFSM 8
>XP_006712160
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   ITGLFTMS 9
           +TGLF+++
Sbjct: 559 VTGLFSLN 566
>XP_006712159
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   ITGLFTMS 9
           +TGLF+++
Sbjct: 831 VTGLFSLN 838
>XP_006712158
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   ITGLFTMS 9
           +TGLF+++
Sbjct: 831 VTGLFSLN 838
>XP_005264603
          Length = 940

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   ITGLFTMS 9
           +TGLF+++
Sbjct: 867 VTGLFSLN 874
>XP_005264601
          Length = 1056

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   ITGLFTMS 9
           +TGLF+++
Sbjct: 983 VTGLFSLN 990
>XP_006712157
          Length = 1071

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    ITGLFTMS 9
            +TGLF+++
Sbjct: 998  VTGLFSLN 1005
>XP_006711427
          Length = 228

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2 ITGLFTM 8
         ITG+F+M
Sbjct: 2 ITGVFSM 8
>XP_005277457
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2 ITGLFTM 8
         ITG+F+M
Sbjct: 2 ITGVFSM 8
>XP_006711426
          Length = 385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2 ITGLFTM 8
         ITG+F+M
Sbjct: 2 ITGVFSM 8
>XP_006710709
          Length = 417

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DITGLFTM 8
           D+T LFT+
Sbjct: 188 DVTFLFTL 195
>XP_001718723
          Length = 1091

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DITGLFTMS 9
           D+T  FT+S
Sbjct: 732 DLTSTFTVS 740
>NP_940857
          Length = 5147

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    DITGLFTMS 9
            + +GLFT+S
Sbjct: 1043 EASGLFTVS 1051
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.140    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 339,604
Number of extensions: 507
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package