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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              19     869
NP_061753                                                              17    4312
NP_001888                                                              16    5631
NP_001376                                                              16    7355
NP_003881                                                              16    7355
NP_057715                                                              15    9605
NP_054722                                                              15    9605
NP_001163935                                                           15    9605
NP_002586                                                              15    9605
XP_006724129                                                           15    9605
XP_005256326                                                           15    9605
XP_006719507                                                           15    9605

>NP_002430
          Length = 1137

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DIKGSQTIS 9
           DIKGSQ IS
Sbjct: 482 DIKGSQIIS 490



>NP_061753
          Length = 800

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   DIKGSQTIS 9
           D++G++T+S
Sbjct: 740 DVRGAETLS 748



>NP_001888
          Length = 2322

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    IKGSQTIS 9
            +KGSQT++
Sbjct: 1584 LKGSQTLT 1591



>NP_001376
          Length = 519

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   DIKGSQTIS 9
           D KG++TIS
Sbjct: 407 DPKGTRTIS 415



>NP_003881
          Length = 5405

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KGSQTIS 9
           +GSQT+S
Sbjct: 912 RGSQTVS 918



>NP_057715
          Length = 917

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DIKGSQTIS 9
           ++KG +TIS
Sbjct: 839 NLKGERTIS 847



>NP_054722
          Length = 1227

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KGSQTI 8
           KGSQT+
Sbjct: 446 KGSQTV 451



>NP_001163935
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  IKGSQTI 8
          ++GSQTI
Sbjct: 12 LRGSQTI 18



>NP_002586
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  IKGSQTI 8
          ++GSQTI
Sbjct: 12 LRGSQTI 18



>XP_006724129
          Length = 882

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DIKGSQTIS 9
           ++KG +TIS
Sbjct: 804 NLKGERTIS 812



>XP_005256326
          Length = 1192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KGSQTI 8
           KGSQT+
Sbjct: 411 KGSQTV 416



>XP_006719507
          Length = 523

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  IKGSQTI 8
          ++GSQTI
Sbjct: 12 LRGSQTI 18



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.301    0.122    0.299 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 499,548
Number of extensions: 582
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)