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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005912                                                              19     665
XP_005248577                                                           19     665
XP_005248576                                                           19     665
XP_006722097                                                           17    2528
NP_001093122                                                           17    4312
XP_005267599                                                           17    4312
NP_680481                                                              16    5631
NP_680480                                                              16    5631
NP_060117                                                              16    5631
NP_001013649                                                           16    5631
XP_006724707                                                           16    5631
XP_005261505                                                           16    5631
XP_006724260                                                           16    5631
XP_005261504                                                           16    5631
NP_055152                                                              16    5631
NP_003283                                                              16    7355
XP_005245528                                                           16    7355
NP_000160                                                              15    9605
NP_001129496                                                           15    9605
NP_938011                                                              15    9605
NP_852003                                                              15    9605
NP_938010                                                              15    9605
NP_938009                                                              15    9605
NP_689909                                                              15    9605
NP_003794                                                              15    9605
NP_062830                                                              15    9605
NP_203127                                                              15    9605
XP_005274596                                                           15    9605
XP_005268366                                                           15    9605
XP_005270670                                                           15    9605
XP_005270669                                                           15    9605
XP_006710506                                                           15    9605
XP_005270668                                                           15    9605
XP_005270667                                                           15    9605
XP_005270666                                                           15    9605

>NP_005912
          Length = 1512

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DIITIQQDT 9
            DII IQQDT
Sbjct: 1215 DIIIIQQDT 1223



>XP_005248577
          Length = 1349

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DIITIQQDT 9
            DII IQQDT
Sbjct: 1052 DIIIIQQDT 1060



>XP_005248576
          Length = 1375

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DIITIQQDT 9
            DII IQQDT
Sbjct: 1078 DIIIIQQDT 1086



>XP_006722097
          Length = 1862

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    DIITIQQDT 9
            DI+T Q+DT
Sbjct: 1390 DILTTQRDT 1398



>NP_001093122
          Length = 429

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IITIQQDT 9
           ++TI QDT
Sbjct: 267 VVTISQDT 274



>XP_005267599
          Length = 230

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  IITIQQDT 9
          ++TI QDT
Sbjct: 68 VVTISQDT 75



>NP_680481
          Length = 527

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DIITIQQDT 9
           DIIT+Q  T
Sbjct: 154 DIITLQDPT 162



>NP_680480
          Length = 520

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DIITIQQDT 9
           DIIT+Q  T
Sbjct: 154 DIITLQDPT 162



>NP_060117
          Length = 847

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  DIITIQQD 8
          +++T+QQD
Sbjct: 85 NVVTLQQD 92



>NP_001013649
          Length = 1225

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    IITIQQDT 9
            IIT+++DT
Sbjct: 1036 IITLEEDT 1043



>XP_006724707
          Length = 1337

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    IITIQQDT 9
            IIT+++DT
Sbjct: 1148 IITLEEDT 1155



>XP_005261505
          Length = 520

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DIITIQQDT 9
           DIIT+Q  T
Sbjct: 154 DIITLQDPT 162



>XP_006724260
          Length = 520

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DIITIQQDT 9
           DIIT+Q  T
Sbjct: 154 DIITLQDPT 162



>XP_005261504
          Length = 520

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DIITIQQDT 9
           DIIT+Q  T
Sbjct: 154 DIITLQDPT 162



>NP_055152
          Length = 520

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DIITIQQDT 9
           DIIT+Q  T
Sbjct: 154 DIITLQDPT 162



>NP_003283
          Length = 2363

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IITIQQDT 9
            I+T+QQ T
Sbjct: 1763 ILTVQQQT 1770



>XP_005245528
          Length = 2439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    IITIQQDT 9
            I+T+QQ T
Sbjct: 1839 ILTVQQQT 1846



>NP_000160
          Length = 429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   DIITIQQD 8
           D+I I QD
Sbjct: 315 DVIAINQD 322



>NP_001129496
          Length = 1474

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    DIITIQQDT 9
            D+IT Q D+
Sbjct: 1144 DVITSQSDS 1152



>NP_938011
          Length = 1630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    DIITIQQDT 9
            D+IT Q D+
Sbjct: 1300 DVITSQSDS 1308



>NP_852003
          Length = 816

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IITIQQDT 9
           +ITI+ DT
Sbjct: 511 VITIRSDT 518



>NP_938010
          Length = 836

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IITIQQDT 9
           +ITI+ DT
Sbjct: 531 VITIRSDT 538



>NP_938009
          Length = 1210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IITIQQDT 9
           +ITI+ DT
Sbjct: 905 VITIRSDT 912



>NP_689909
          Length = 1075

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IITIQQDT 9
           +ITI+ DT
Sbjct: 905 VITIRSDT 912



>NP_003794
          Length = 1685

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    DIITIQQ 7
            D++TIQ+
Sbjct: 1580 DVVTIQE 1586



>NP_062830
          Length = 1589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    DIITIQQ 7
            D++TIQ+
Sbjct: 1484 DVVTIQE 1490



>NP_203127
          Length = 236

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   DIITIQQDT 9
           D+++ Q+DT
Sbjct: 152 DLVSAQKDT 160



>XP_005274596
          Length = 1651

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    DIITIQQDT 9
            D+IT Q D+
Sbjct: 1321 DVITSQSDS 1329



>XP_005268366
          Length = 1180

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  DIITIQQDT 9
          D+I + QDT
Sbjct: 40 DVIRLVQDT 48



>XP_005270670
          Length = 1210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IITIQQDT 9
           +ITI+ DT
Sbjct: 905 VITIRSDT 912



>XP_005270669
          Length = 1210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IITIQQDT 9
           +ITI+ DT
Sbjct: 905 VITIRSDT 912



>XP_006710506
          Length = 1247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IITIQQDT 9
           +ITI+ DT
Sbjct: 942 VITIRSDT 949



>XP_005270668
          Length = 1256

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IITIQQDT 9
           +ITI+ DT
Sbjct: 976 VITIRSDT 983



>XP_005270667
          Length = 1280

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IITIQQDT 9
           +ITI+ DT
Sbjct: 975 VITIRSDT 982



>XP_005270666
          Length = 1281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IITIQQDT 9
           +ITI+ DT
Sbjct: 976 VITIRSDT 983



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.137    0.348 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 475,950
Number of extensions: 1146
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)