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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           19     665
NP_000242                                                              19     665
XP_005264389                                                           19     665
NP_055847                                                              17    2528
NP_443148                                                              17    2528
XP_006721725                                                           17    2528
XP_005257064                                                           17    2528
XP_006721724                                                           17    2528
XP_006721723                                                           17    2528
XP_005266355                                                           17    2528
XP_005266354                                                           17    2528
XP_005266353                                                           17    2528
XP_005266352                                                           17    2528
NP_003923                                                              16    7355
NP_001265518                                                           16    7355
NP_001129061                                                           16    7355
NP_060156                                                              16    7355
XP_005271016                                                           16    7355
NP_004456                                                              15    9605
NP_001093869                                                           15    9605
NP_001093870                                                           15    9605
NP_006575                                                              15    9605
NP_001180459                                                           15    9605
NP_001180458                                                           15    9605
NP_665803                                                              15    9605
XP_006721704                                                           15    9605
XP_005248321                                                           15    9605
XP_005262709                                                           15    9605

>NP_001245210
          Length = 868

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DIAAVKALN 9
           DIAAV+ALN
Sbjct: 237 DIAAVRALN 245



>NP_000242
          Length = 934

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DIAAVKALN 9
           DIAAV+ALN
Sbjct: 303 DIAAVRALN 311



>XP_005264389
          Length = 921

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DIAAVKALN 9
           DIAAV+ALN
Sbjct: 303 DIAAVRALN 311



>NP_055847
          Length = 1447

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   DIAAVKALN 9
           D+ AVKALN
Sbjct: 485 DLNAVKALN 493



>NP_443148
          Length = 679

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   DIAAVKALN 9
           DIA+VKAL+
Sbjct: 622 DIASVKALD 630



>XP_006721725
          Length = 613

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   DIAAVKALN 9
           DIA+VKAL+
Sbjct: 556 DIASVKALD 564



>XP_005257064
          Length = 653

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   DIAAVKALN 9
           DIA+VKAL+
Sbjct: 622 DIASVKALD 630



>XP_006721724
          Length = 654

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   DIAAVKALN 9
           DIA+VKAL+
Sbjct: 623 DIASVKALD 631



>XP_006721723
          Length = 680

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   DIAAVKALN 9
           DIA+VKAL+
Sbjct: 623 DIASVKALD 631



>XP_005266355
          Length = 1448

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   DIAAVKALN 9
           D+ AVKALN
Sbjct: 485 DLNAVKALN 493



>XP_005266354
          Length = 1411

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   DIAAVKALN 9
           D+ AVKALN
Sbjct: 485 DLNAVKALN 493



>XP_005266353
          Length = 1445

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   DIAAVKALN 9
           D+ AVKALN
Sbjct: 485 DLNAVKALN 493



>XP_005266352
          Length = 1446

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   DIAAVKALN 9
           D+ AVKALN
Sbjct: 485 DLNAVKALN 493



>NP_003923
          Length = 369

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   IAAVKALN 9
           +AA++ALN
Sbjct: 119 VAAIEALN 126



>NP_001265518
          Length = 359

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   IAAVKALN 9
           +AA++ALN
Sbjct: 109 VAAIEALN 116



>NP_001129061
          Length = 496

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   DIAAVKAL 8
           DIA++KAL
Sbjct: 333 DIASLKAL 340



>NP_060156
          Length = 497

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   DIAAVKAL 8
           DIA++KAL
Sbjct: 333 DIASLKAL 340



>XP_005271016
          Length = 519

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   DIAAVKAL 8
           DIA++KAL
Sbjct: 333 DIASLKAL 340



>NP_004456
          Length = 208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IAAVKALN 9
           + AVKA+N
Sbjct: 120 VVAVKAIN 127



>NP_001093869
          Length = 1337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   DIAAVKALN 9
           D  AVKALN
Sbjct: 495 DPNAVKALN 503



>NP_001093870
          Length = 600

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   DIAAVKALN 9
           D  AVKALN
Sbjct: 495 DPNAVKALN 503



>NP_006575
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2 IAAVKALN 9
         +AA+KA+N
Sbjct: 1 MAAIKAVN 8



>NP_001180459
          Length = 485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2 IAAVKALN 9
         +AA+KA+N
Sbjct: 1 MAAIKAVN 8



>NP_001180458
          Length = 493

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2 IAAVKALN 9
         +AA+KA+N
Sbjct: 1 MAAIKAVN 8



>NP_665803
          Length = 1025

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IAAVKALN 9
           +AAVKAL+
Sbjct: 176 LAAVKALS 183



>XP_006721704
          Length = 516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2 IAAVKALN 9
         +AA+KA+N
Sbjct: 1 MAAIKAVN 8



>XP_005248321
          Length = 208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IAAVKALN 9
           + AVKA+N
Sbjct: 120 VVAVKAIN 127



>XP_005262709
          Length = 1337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   DIAAVKALN 9
           D  AVKALN
Sbjct: 495 DPNAVKALN 503



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.127    0.309 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 209,405
Number of extensions: 381
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)