Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              22     103
NP_001075109                                                           22     103
NP_663782                                                              17    4312
NP_001118                                                              17    4312
NP_001159491                                                           17    4312
NP_112224                                                              16    5631
NP_060626                                                              16    5631
NP_056193                                                              16    5631
NP_056415                                                              16    5631
NP_001035937                                                           16    5631
XP_005253212                                                           16    5631
XP_005253211                                                           16    5631
NP_003195                                                              15    9605
NP_078863                                                              15    9605
NP_055178                                                              15    9605
NP_003244                                                              15    9605
NP_001264984                                                           15    9605
NP_006043                                                              15    9605
NP_078881                                                              15    9605
XP_005261097                                                           15    9605
XP_005261096                                                           15    9605
XP_005261095                                                           15    9605
XP_005261094                                                           15    9605
XP_005260968                                                           15    9605
XP_005260966                                                           15    9605
XP_005257472                                                           15    9605
XP_005257470                                                           15    9605
XP_005257469                                                           15    9605
XP_005257468                                                           15    9605
XP_005257467                                                           15    9605
XP_005257995                                                           15    9605
XP_005257994                                                           15    9605
XP_005266395                                                           15    9605
XP_005263274                                                           15    9605
XP_005263272                                                           15    9605
XP_005263271                                                           15    9605
XP_005271253                                                           15    9605
XP_005271252                                                           15    9605
XP_005271251                                                           15    9605
NP_001273                                                              15    9605
NP_001025177                                                           15    9605
>NP_008835
          Length = 4128

 Score = 21.9 bits (45), Expect =   103
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    DFKVEFMRN 9
            DFKVEF+RN
Sbjct: 3689 DFKVEFLRN 3697
>NP_001075109
          Length = 4097

 Score = 21.9 bits (45), Expect =   103
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    DFKVEFMRN 9
            DFKVEF+RN
Sbjct: 3689 DFKVEFLRN 3697
>NP_663782
          Length = 939

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DFKVEFMRN 9
           DF ++F RN
Sbjct: 754 DFAIQFNRN 762
>NP_001118
          Length = 949

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DFKVEFMRN 9
           DF ++F RN
Sbjct: 761 DFAIQFNRN 769
>NP_001159491
          Length = 919

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DFKVEFMRN 9
           DF ++F RN
Sbjct: 734 DFAIQFNRN 742
>NP_112224
          Length = 1849

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    DFKVEFMRN 9
            +FKVEF  N
Sbjct: 1327 NFKVEFALN 1335
>NP_060626
          Length = 4363

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    DFKVEFMRN 9
            DF+VEF ++
Sbjct: 1649 DFEVEFSKD 1657
>NP_056193
          Length = 4388

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    DFKVEFMRN 9
            DF+VEF ++
Sbjct: 1649 DFEVEFSKD 1657
>NP_056415
          Length = 404

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DFKVEFM 7
           DFKV+F+
Sbjct: 356 DFKVQFV 362
>NP_001035937
          Length = 398

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DFKVEFM 7
           DFKV+F+
Sbjct: 356 DFKVQFV 362
>XP_005253212
          Length = 1838

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    DFKVEFMRN 9
            +FKVEF  N
Sbjct: 1316 NFKVEFALN 1324
>XP_005253211
          Length = 1881

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    DFKVEFMRN 9
            +FKVEF  N
Sbjct: 1359 NFKVEFALN 1367
>NP_003195
          Length = 772

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KVEFMRN 9
           KVEF+R+
Sbjct: 700 KVEFLRS 706
>NP_078863
          Length = 277

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VEFMRN 9
           V+FMRN
Sbjct: 268 VDFMRN 273
>NP_055178
          Length = 4579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    VEFMRN 9
            VEF+RN
Sbjct: 3583 VEFLRN 3588
>NP_003244
          Length = 1591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    DFKVEFMR 8
            +F+VEF++
Sbjct: 1093 EFQVEFLK 1100
>NP_001264984
          Length = 4432

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    VEFMRN 9
            VEF+RN
Sbjct: 3436 VEFLRN 3441
>NP_006043
          Length = 297

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DFKVEFMRN 9
           +FKVEF  N
Sbjct: 267 NFKVEFEVN 275
>NP_078881
          Length = 786

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DFKVEFM 7
          DFK EF+
Sbjct: 66 DFKFEFI 72
>XP_005261097
          Length = 1452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    DFKVEFMR 8
            +F+VEF++
Sbjct: 1093 EFQVEFLK 1100
>XP_005261096
          Length = 1591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    DFKVEFMR 8
            +F+VEF++
Sbjct: 1093 EFQVEFLK 1100
>XP_005261095
          Length = 1591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    DFKVEFMR 8
            +F+VEF++
Sbjct: 1093 EFQVEFLK 1100
>XP_005261094
          Length = 1591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    DFKVEFMR 8
            +F+VEF++
Sbjct: 1093 EFQVEFLK 1100
>XP_005260968
          Length = 172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DFKVEFMRN 9
           +FKVEF  N
Sbjct: 142 NFKVEFEVN 150
>XP_005260966
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DFKVEFMRN 9
           +FKVEF  N
Sbjct: 240 NFKVEFEVN 248
>XP_005257472
          Length = 731

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KVEFMRN 9
           KVEF+R+
Sbjct: 659 KVEFLRS 665
>XP_005257470
          Length = 742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KVEFMRN 9
           KVEF+R+
Sbjct: 670 KVEFLRS 676
>XP_005257469
          Length = 742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KVEFMRN 9
           KVEF+R+
Sbjct: 670 KVEFLRS 676
>XP_005257468
          Length = 761

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KVEFMRN 9
           KVEF+R+
Sbjct: 689 KVEFLRS 695
>XP_005257467
          Length = 772

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KVEFMRN 9
           KVEF+R+
Sbjct: 700 KVEFLRS 706
>XP_005257995
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   DFKVEFMRN 9
           DF ++F +N
Sbjct: 766 DFAIQFNKN 774
>XP_005257994
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   DFKVEFMRN 9
           DF ++F +N
Sbjct: 766 DFAIQFNKN 774
>XP_005266395
          Length = 4588

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    VEFMRN 9
            VEF+RN
Sbjct: 3592 VEFLRN 3597
>XP_005263274
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DFKVEFM 7
          DFK EF+
Sbjct: 66 DFKFEFI 72
>XP_005263272
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DFKVEFM 7
          DFK EF+
Sbjct: 66 DFKFEFI 72
>XP_005263271
          Length = 762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  DFKVEFM 7
          DFK EF+
Sbjct: 66 DFKFEFI 72
>XP_005271253
          Length = 204

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VEFMRN 9
           V+FMRN
Sbjct: 195 VDFMRN 200
>XP_005271252
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VEFMRN 9
           V+FMRN
Sbjct: 237 VDFMRN 242
>XP_005271251
          Length = 247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VEFMRN 9
           V+FMRN
Sbjct: 238 VDFMRN 243
>NP_001273
          Length = 937

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   DFKVEFMRN 9
           DF ++F +N
Sbjct: 752 DFAIQFNKN 760
>NP_001025177
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   DFKVEFMRN 9
           DF ++F +N
Sbjct: 766 DFAIQFNKN 774
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.147    0.433 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 419,710
Number of extensions: 713
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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