Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     299
XP_005262424                                                           20     299
XP_006723519                                                           18    1935
XP_006723518                                                           18    1935
XP_006723517                                                           18    1935
NP_149074                                                              17    3301
NP_002227                                                              17    4312
NP_000103                                                              16    7355
NP_061992                                                              16    7355
NP_003861                                                              16    7355
NP_598376                                                              16    7355
NP_839943                                                              16    7355
NP_001080                                                              15    9605
NP_653223                                                              15    9605
NP_003310                                                              15    9605
NP_597676                                                              15    9605
NP_596870                                                              15    9605
NP_001243779                                                           15    9605
NP_596869                                                              15    9605
NP_001240841                                                           15    9605
NP_055988                                                              15    9605
NP_001077086                                                           15    9605
NP_055596                                                              15    9605
XP_005257995                                                           15    9605
XP_005257994                                                           15    9605
XP_006716556                                                           15    9605
XP_006716555                                                           15    9605
XP_006716554                                                           15    9605
XP_005262708                                                           15    9605
XP_005262706                                                           15    9605
XP_006714063                                                           15    9605
XP_005262705                                                           15    9605
XP_006714062                                                           15    9605
XP_005262704                                                           15    9605
XP_005262703                                                           15    9605
XP_006712787                                                           15    9605
XP_006712786                                                           15    9605
NP_001273                                                              15    9605
NP_001025177                                                           15    9605
NP_001254479                                                           15    9605
NP_597681                                                              15    9605
>NP_722576
          Length = 3080

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DEISMTLTH 9
            DEISM LTH
Sbjct: 2504 DEISMNLTH 2512
>XP_005262424
          Length = 2875

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DEISMTLTH 9
            DEISM LTH
Sbjct: 2299 DEISMNLTH 2307
>XP_006723519
          Length = 223

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DEISMTLTH 9
           D +SMT TH
Sbjct: 182 DTLSMTFTH 190
>XP_006723518
          Length = 277

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DEISMTLTH 9
           D +SMT TH
Sbjct: 236 DTLSMTFTH 244
>XP_006723517
          Length = 327

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DEISMTLTH 9
           D +SMT TH
Sbjct: 286 DTLSMTFTH 294
>NP_149074
          Length = 268

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DEISMTLTH 9
          DE+++TL H
Sbjct: 56 DEVTITLPH 64
>NP_002227
          Length = 494

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ISMTLTH 9
           +S+TLTH
Sbjct: 268 VSLTLTH 274
>NP_000103
          Length = 739

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DEISMTLTH 9
           DE+S+ L+H
Sbjct: 633 DEMSVQLSH 641
>NP_061992
          Length = 797

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   DEISMTLT 8
           +E+S+TLT
Sbjct: 206 EELSLTLT 213
>NP_003861
          Length = 1657

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    EISMTLTH 9
            E+S+TLT+
Sbjct: 1360 EVSLTLTN 1367
>NP_598376
          Length = 717

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DEISMTLTH 9
           DE+ +TL H
Sbjct: 117 DEMDLTLGH 125
>NP_839943
          Length = 1631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    EISMTLTH 9
            E+S+TLT+
Sbjct: 1341 EVSLTLTN 1348
>NP_001080
          Length = 1704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    DEISMTLTH 9
            +E+S TL H
Sbjct: 1239 EELSKTLDH 1247
>NP_653223
          Length = 773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   EISMTLTH 9
           E +MT TH
Sbjct: 282 EFNMTATH 289
>NP_003310
          Length = 26926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    DEISMTLT 8
            D++S TLT
Sbjct: 2912 DQVSATLT 2919
>NP_597676
          Length = 27051

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    DEISMTLT 8
            D++S TLT
Sbjct: 2912 DQVSATLT 2919
>NP_596870
          Length = 5604

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    DEISMTLT 8
            D++S TLT
Sbjct: 2958 DQVSATLT 2965
>NP_001243779
          Length = 34350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    DEISMTLT 8
            D++S TLT
Sbjct: 2958 DQVSATLT 2965
>NP_596869
          Length = 33423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    DEISMTLT 8
            D++S TLT
Sbjct: 2958 DQVSATLT 2965
>NP_001240841
          Length = 1159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   DEISMTL 7
           DEI MTL
Sbjct: 361 DEIMMTL 367
>NP_055988
          Length = 1168

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   DEISMTL 7
           DEI MTL
Sbjct: 361 DEIMMTL 367
>NP_001077086
          Length = 1591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DEISMTLT 8
           DE+S T+T
Sbjct: 626 DEMSQTIT 633
>NP_055596
          Length = 1594

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DEISMTLT 8
           DE+S T+T
Sbjct: 626 DEMSQTIT 633
>XP_005257995
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EISMTLTH 9
           EIS T TH
Sbjct: 738 EISGTFTH 745
>XP_005257994
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EISMTLTH 9
           EIS T TH
Sbjct: 738 EISGTFTH 745
>XP_006716556
          Length = 1048

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  DEISMTLT 8
          DE+S T+T
Sbjct: 83 DEMSQTIT 90
>XP_006716555
          Length = 1539

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DEISMTLT 8
           DE+S T+T
Sbjct: 574 DEMSQTIT 581
>XP_006716554
          Length = 1591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DEISMTLT 8
           DE+S T+T
Sbjct: 626 DEMSQTIT 633
>XP_005262708
          Length = 1039

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   DEISMTL 7
           DEI MTL
Sbjct: 232 DEIMMTL 238
>XP_005262706
          Length = 1146

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   DEISMTL 7
           DEI MTL
Sbjct: 232 DEIMMTL 238
>XP_006714063
          Length = 1168

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   DEISMTL 7
           DEI MTL
Sbjct: 361 DEIMMTL 367
>XP_005262705
          Length = 1181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   DEISMTL 7
           DEI MTL
Sbjct: 361 DEIMMTL 367
>XP_006714062
          Length = 1262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   DEISMTL 7
           DEI MTL
Sbjct: 361 DEIMMTL 367
>XP_005262704
          Length = 1262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   DEISMTL 7
           DEI MTL
Sbjct: 361 DEIMMTL 367
>XP_005262703
          Length = 1275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   DEISMTL 7
           DEI MTL
Sbjct: 361 DEIMMTL 367
>XP_006712787
          Length = 33978

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    DEISMTLT 8
            D++S TLT
Sbjct: 2959 DQVSATLT 2966
>XP_006712786
          Length = 35484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    DEISMTLT 8
            D++S TLT
Sbjct: 2959 DQVSATLT 2966
>NP_001273
          Length = 937

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EISMTLTH 9
           EIS T TH
Sbjct: 724 EISGTFTH 731
>NP_001025177
          Length = 951

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EISMTLTH 9
           EIS T TH
Sbjct: 738 EISGTFTH 745
>NP_001254479
          Length = 35991

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    DEISMTLT 8
            D++S TLT
Sbjct: 2958 DQVSATLT 2965
>NP_597681
          Length = 27118

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    DEISMTLT 8
            D++S TLT
Sbjct: 2912 DQVSATLT 2919
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.122    0.327 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 407,267
Number of extensions: 897
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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