Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001243398                                                           21     229
NP_036462                                                              21     229
NP_001243397                                                           21     229
XP_005269721                                                           21     229
NP_000386                                                              18    1935
XP_005261397                                                           18    1935
NP_000085                                                              17    2528
NP_003820                                                              17    2528
NP_001248335                                                           17    2528
NP_001248336                                                           17    2528
XP_005251680                                                           17    2528
XP_006716954                                                           17    2528
XP_006716953                                                           17    2528
XP_006716952                                                           17    2528
XP_006716951                                                           17    2528
XP_006716950                                                           17    2528
XP_006716949                                                           17    2528
XP_006716948                                                           17    2528
XP_005251679                                                           17    2528
NP_005090                                                              17    4312
NP_001230675                                                           17    4312
NP_060035                                                              17    4312
XP_006722023                                                           17    4312
XP_006722022                                                           17    4312
XP_006722021                                                           17    4312
XP_006711698                                                           17    4312
NP_005535                                                              16    5631
XP_006712575                                                           16    5631
XP_005246591                                                           16    5631
NP_620686                                                              16    7355
XP_005271476                                                           16    7355
XP_006718836                                                           16    7355
NP_008919                                                              15    9605
NP_001153059                                                           15    9605
NP_001153060                                                           15    9605
NP_598000                                                              15    9605
NP_064608                                                              15    9605
NP_001157968                                                           15    9605
XP_005249871                                                           15    9605
XP_005248716                                                           15    9605
XP_006713762                                                           15    9605
XP_006713759                                                           15    9605
NP_060808                                                              15    9605
>NP_001243398
          Length = 1781

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DEGSMSAFT 9
           DEGSM+AFT
Sbjct: 359 DEGSMNAFT 367
>NP_036462
          Length = 2073

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DEGSMSAFT 9
           DEGSM+AFT
Sbjct: 359 DEGSMNAFT 367
>NP_001243397
          Length = 1890

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DEGSMSAFT 9
           DEGSM+AFT
Sbjct: 359 DEGSMNAFT 367
>XP_005269721
          Length = 2073

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DEGSMSAFT 9
           DEGSM+AFT
Sbjct: 359 DEGSMNAFT 367
>NP_000386
          Length = 897

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   GSMSAFT 9
           GSMSAFT
Sbjct: 498 GSMSAFT 504
>XP_005261397
          Length = 903

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   GSMSAFT 9
           GSMSAFT
Sbjct: 504 GSMSAFT 510
>NP_000085
          Length = 2944

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    DEGSMSAFT 9
            DEGS +A+T
Sbjct: 2881 DEGSCTAYT 2889
>NP_003820
          Length = 2041

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    EGSMSAFT 9
            EGS+S+FT
Sbjct: 1796 EGSLSSFT 1803
>NP_001248335
          Length = 2037

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    EGSMSAFT 9
            EGS+S+FT
Sbjct: 1792 EGSLSSFT 1799
>NP_001248336
          Length = 2008

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    EGSMSAFT 9
            EGS+S+FT
Sbjct: 1763 EGSLSSFT 1770
>XP_005251680
          Length = 2000

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    EGSMSAFT 9
            EGS+S+FT
Sbjct: 1755 EGSLSSFT 1762
>XP_006716954
          Length = 2008

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    EGSMSAFT 9
            EGS+S+FT
Sbjct: 1763 EGSLSSFT 1770
>XP_006716953
          Length = 2031

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    EGSMSAFT 9
            EGS+S+FT
Sbjct: 1786 EGSLSSFT 1793
>XP_006716952
          Length = 2037

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    EGSMSAFT 9
            EGS+S+FT
Sbjct: 1792 EGSLSSFT 1799
>XP_006716951
          Length = 2041

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    EGSMSAFT 9
            EGS+S+FT
Sbjct: 1796 EGSLSSFT 1803
>XP_006716950
          Length = 2070

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    EGSMSAFT 9
            EGS+S+FT
Sbjct: 1825 EGSLSSFT 1832
>XP_006716949
          Length = 2070

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    EGSMSAFT 9
            EGS+S+FT
Sbjct: 1825 EGSLSSFT 1832
>XP_006716948
          Length = 2070

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    EGSMSAFT 9
            EGS+S+FT
Sbjct: 1825 EGSLSSFT 1832
>XP_005251679
          Length = 2070

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    EGSMSAFT 9
            EGS+S+FT
Sbjct: 1825 EGSLSSFT 1832
>NP_005090
          Length = 837

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DEGSMSAFT 9
           D+G  SAFT
Sbjct: 350 DDGLQSAFT 358
>NP_001230675
          Length = 403

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EGSMSAF 8
           EGS+SAF
Sbjct: 199 EGSLSAF 205
>NP_060035
          Length = 541

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EGSMSAF 8
           EGS+SAF
Sbjct: 337 EGSLSAF 343
>XP_006722023
          Length = 386

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EGSMSAF 8
           EGS+SAF
Sbjct: 337 EGSLSAF 343
>XP_006722022
          Length = 403

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EGSMSAF 8
           EGS+SAF
Sbjct: 199 EGSLSAF 205
>XP_006722021
          Length = 418

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   EGSMSAF 8
           EGS+SAF
Sbjct: 337 EGSLSAF 343
>XP_006711698
          Length = 846

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DEGSMSAFT 9
           D+G  SAFT
Sbjct: 350 DDGLQSAFT 358
>NP_005535
          Length = 1242

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    GSMSAFT 9
            G MSAFT
Sbjct: 1067 GGMSAFT 1073
>XP_006712575
          Length = 1242

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    GSMSAFT 9
            G MSAFT
Sbjct: 1067 GGMSAFT 1073
>XP_005246591
          Length = 1242

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    GSMSAFT 9
            G MSAFT
Sbjct: 1067 GGMSAFT 1073
>NP_620686
          Length = 950

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DEGSMSAFT 9
           D+G  SAFT
Sbjct: 350 DDGLPSAFT 358
>XP_005271476
          Length = 619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DEGSMSAFT 9
          D+G  SAFT
Sbjct: 19 DDGLPSAFT 27
>XP_006718836
          Length = 642

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DEGSMSAFT 9
          D+G  SAFT
Sbjct: 42 DDGLPSAFT 50
>NP_008919
          Length = 967

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DEGSMSAFT 9
           D+G  +AFT
Sbjct: 390 DDGLQAAFT 398
>NP_001153059
          Length = 1428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DEGSMSAF 8
           ++GS SAF
Sbjct: 970 EDGSFSAF 977
>NP_001153060
          Length = 1368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DEGSMSAF 8
           ++GS SAF
Sbjct: 893 EDGSFSAF 900
>NP_598000
          Length = 1445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DEGSMSAF 8
           ++GS SAF
Sbjct: 970 EDGSFSAF 977
>NP_064608
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   EGSMSAF 8
           EGS++AF
Sbjct: 385 EGSLAAF 391
>NP_001157968
          Length = 1854

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  EGSMSAF 8
          EGS+S+F
Sbjct: 69 EGSLSSF 75
>XP_005249871
          Length = 252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  EGSMSAF 8
          EGS++AF
Sbjct: 53 EGSLAAF 59
>XP_005248716
          Length = 1445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DEGSMSAF 8
           ++GS SAF
Sbjct: 970 EDGSFSAF 977
>XP_006713762
          Length = 1197

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   EGSMSAF 8
           EGS+S+F
Sbjct: 113 EGSLSSF 119
>XP_006713759
          Length = 1898

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   EGSMSAF 8
           EGS+S+F
Sbjct: 113 EGSLSSF 119
>NP_060808
          Length = 982

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  EGSMSAF 8
          EGS+S+F
Sbjct: 69 EGSLSSF 75
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.304    0.116    0.303 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 497,274
Number of extensions: 773
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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