Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              19     665
NP_653080                                                              19     665
XP_005267292                                                           19     665
XP_005267291                                                           19     665
XP_005267290                                                           19     665
NP_061130                                                              17    3301
NP_055093                                                              17    4312
NP_001182325                                                           17    4312
NP_997625                                                              17    4312
NP_001032241                                                           17    4312
NP_005729                                                              17    4312
NP_001073280                                                           16    5631
NP_005210                                                              16    5631
XP_005268441                                                           16    5631
NP_000060                                                              16    7355
NP_001231118                                                           16    7355
NP_001231121                                                           16    7355
NP_055564                                                              16    7355
NP_001231119                                                           16    7355
NP_001231120                                                           16    7355
NP_001231122                                                           16    7355
NP_004378                                                              16    7355
NP_056355                                                              16    7355
NP_004609                                                              16    7355
NP_006746                                                              16    7355
XP_005256833                                                           16    7355
XP_006720533                                                           16    7355
XP_006720532                                                           16    7355
XP_005254354                                                           16    7355
XP_006720406                                                           16    7355
XP_006720405                                                           16    7355
XP_006720404                                                           16    7355
XP_006720403                                                           16    7355
XP_006720402                                                           16    7355
XP_006720400                                                           16    7355
XP_006720399                                                           16    7355
XP_005265877                                                           16    7355
XP_005245535                                                           16    7355
XP_005245161                                                           16    7355
NP_037485                                                              16    7355
NP_055136                                                              15    9605
>NP_005091
          Length = 1782

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DAVEMEKME 9
           DAVE EKME
Sbjct: 853 DAVEREKME 861
>NP_653080
          Length = 1684

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DAVEMEKME 9
           DAVE EKME
Sbjct: 755 DAVEREKME 763
>XP_005267292
          Length = 1677

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DAVEMEKME 9
           DAVE EKME
Sbjct: 748 DAVEREKME 756
>XP_005267291
          Length = 1684

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DAVEMEKME 9
           DAVE EKME
Sbjct: 755 DAVEREKME 763
>XP_005267290
          Length = 1782

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DAVEMEKME 9
           DAVE EKME
Sbjct: 853 DAVEREKME 861
>NP_061130
          Length = 513

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   DAVEMEKM 8
           DAVEM++M
Sbjct: 161 DAVEMDEM 168
>NP_055093
          Length = 839

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   DAVEMEKME 9
           D  EMEK+E
Sbjct: 730 DPTEMEKVE 738
>NP_001182325
          Length = 200

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   DAVEMEKME 9
           D+V++E+ME
Sbjct: 101 DSVDVERME 109
>NP_997625
          Length = 200

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   DAVEMEKME 9
           D+V++E+ME
Sbjct: 101 DSVDVERME 109
>NP_001032241
          Length = 200

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   DAVEMEKME 9
           D+V++E+ME
Sbjct: 101 DSVDVERME 109
>NP_005729
          Length = 200

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   DAVEMEKME 9
           D+V++E+ME
Sbjct: 101 DSVDVERME 109
>NP_001073280
          Length = 1263

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VEMEKME 9
           VEM+KME
Sbjct: 491 VEMKKME 497
>NP_005210
          Length = 1272

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VEMEKME 9
           VEM+KME
Sbjct: 500 VEMKKME 506
>XP_005268441
          Length = 1250

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VEMEKME 9
           VEM+KME
Sbjct: 500 VEMKKME 506
>NP_000060
          Length = 1873

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    VEMEKME 9
            +EME+ME
Sbjct: 1633 IEMEEME 1639
>NP_001231118
          Length = 1644

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    DAVEME 6
            DAVEME
Sbjct: 1335 DAVEME 1340
>NP_001231121
          Length = 1504

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    DAVEME 6
            DAVEME
Sbjct: 1238 DAVEME 1243
>NP_055564
          Length = 1472

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    DAVEME 6
            DAVEME
Sbjct: 1206 DAVEME 1211
>NP_001231119
          Length = 1533

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    DAVEME 6
            DAVEME
Sbjct: 1267 DAVEME 1272
>NP_001231120
          Length = 1463

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    DAVEME 6
            DAVEME
Sbjct: 1197 DAVEME 1202
>NP_001231122
          Length = 1347

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    DAVEME 6
            DAVEME
Sbjct: 1142 DAVEME 1147
>NP_004378
          Length = 324

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AVEMEKME 9
           AVE+EK E
Sbjct: 119 AVELEKTE 126
>NP_056355
          Length = 1393

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DAVEMEKM 8
           D VE+EK+
Sbjct: 330 DTVELEKL 337
>NP_004609
          Length = 1001

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DAVEMEKM 8
           D VE+EK+
Sbjct: 328 DTVELEKL 335
>NP_006746
          Length = 337

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   DAVEMEKM 8
           DAV++E+M
Sbjct: 318 DAVKLERM 325
>XP_005256833
          Length = 906

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DAVEMEKM 8
           D VE+EK+
Sbjct: 233 DTVELEKL 240
>XP_006720533
          Length = 1098

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  DAVEMEKM 8
          D VE+EK+
Sbjct: 35 DTVELEKL 42
>XP_006720532
          Length = 1333

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DAVEMEKM 8
           D VE+EK+
Sbjct: 270 DTVELEKL 277
>XP_005254354
          Length = 1393

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DAVEMEKM 8
           D VE+EK+
Sbjct: 330 DTVELEKL 337
>XP_006720406
          Length = 1591

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    DAVEME 6
            DAVEME
Sbjct: 1282 DAVEME 1287
>XP_006720405
          Length = 1347

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    DAVEME 6
            DAVEME
Sbjct: 1282 DAVEME 1287
>XP_006720404
          Length = 1477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    DAVEME 6
            DAVEME
Sbjct: 1282 DAVEME 1287
>XP_006720403
          Length = 1486

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    DAVEME 6
            DAVEME
Sbjct: 1282 DAVEME 1287
>XP_006720402
          Length = 1487

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    DAVEME 6
            DAVEME
Sbjct: 1282 DAVEME 1287
>XP_006720400
          Length = 1526

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    DAVEME 6
            DAVEME
Sbjct: 1282 DAVEME 1287
>XP_006720399
          Length = 1548

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1    DAVEME 6
            DAVEME
Sbjct: 1282 DAVEME 1287
>XP_005265877
          Length = 286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AVEMEKME 9
          AVE+EK E
Sbjct: 81 AVELEKTE 88
>XP_005245535
          Length = 1854

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    VEMEKME 9
            +EME+ME
Sbjct: 1614 IEMEEME 1620
>XP_005245161
          Length = 306

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DAVEME 6
           DAVEME
Sbjct: 257 DAVEME 262
>NP_037485
          Length = 345

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DAVEME 6
           DAVEME
Sbjct: 296 DAVEME 301
>NP_055136
          Length = 252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AVEMEKME 9
           A EMEK+E
Sbjct: 209 AKEMEKVE 216
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.298    0.115    0.266 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 457,172
Number of extensions: 866
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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