Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              20     509
NP_444254                                                              20     509
NP_444253                                                              20     509
NP_444255                                                              20     509
XP_005247551                                                           20     509
XP_005247550                                                           20     509
XP_006713712                                                           20     509
XP_005247549                                                           20     509
NP_001238981                                                           17    3301
NP_001238978                                                           17    3301
NP_001238979                                                           17    3301
NP_001238980                                                           17    3301
NP_001238982                                                           17    3301
NP_005380                                                              17    3301
NP_065744                                                              16    5631
NP_004490                                                              16    5631
NP_112556                                                              16    5631
NP_079321                                                              16    7355
NP_001257475                                                           16    7355
NP_061330                                                              16    7355
NP_443122                                                              16    7355
NP_619636                                                              16    7355
XP_005261808                                                           16    7355
XP_005254913                                                           16    7355
NP_733751                                                              15    9605
NP_001071107                                                           15    9605
NP_060148                                                              15    9605
XP_006716142                                                           15    9605
XP_005250088                                                           15    9605
XP_006716141                                                           15    9605
XP_005250085                                                           15    9605
XP_006716140                                                           15    9605
XP_005250084                                                           15    9605
XP_005250083                                                           15    9605
XP_005250082                                                           15    9605
NP_997204                                                              15    9605
>NP_444256
          Length = 1794

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DATVKMKPA 9
            DATVK KPA
Sbjct: 1150 DATVKKKPA 1158
>NP_444254
          Length = 1845

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DATVKMKPA 9
            DATVK KPA
Sbjct: 1150 DATVKKKPA 1158
>NP_444253
          Length = 1914

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DATVKMKPA 9
            DATVK KPA
Sbjct: 1219 DATVKKKPA 1227
>NP_444255
          Length = 1863

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DATVKMKPA 9
            DATVK KPA
Sbjct: 1219 DATVKKKPA 1227
>XP_005247551
          Length = 1855

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DATVKMKPA 9
            DATVK KPA
Sbjct: 1160 DATVKKKPA 1168
>XP_005247550
          Length = 1856

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DATVKMKPA 9
            DATVK KPA
Sbjct: 1229 DATVKKKPA 1237
>XP_006713712
          Length = 1923

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DATVKMKPA 9
            DATVK KPA
Sbjct: 1229 DATVKKKPA 1237
>XP_005247549
          Length = 1924

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DATVKMKPA 9
            DATVK KPA
Sbjct: 1229 DATVKKKPA 1237
>NP_001238981
          Length = 251

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DATVKMKP 8
           D ++KMKP
Sbjct: 223 DGSIKMKP 230
>NP_001238978
          Length = 286

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DATVKMKP 8
           D ++KMKP
Sbjct: 258 DGSIKMKP 265
>NP_001238979
          Length = 250

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DATVKMKP 8
           D ++KMKP
Sbjct: 223 DGSIKMKP 230
>NP_001238980
          Length = 250

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DATVKMKP 8
           D ++KMKP
Sbjct: 223 DGSIKMKP 230
>NP_001238982
          Length = 285

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DATVKMKP 8
           D ++KMKP
Sbjct: 258 DGSIKMKP 265
>NP_005380
          Length = 285

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DATVKMKP 8
           D ++KMKP
Sbjct: 258 DGSIKMKP 265
>NP_065744
          Length = 2113

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ATVKMKPA 9
           +T+K+KPA
Sbjct: 910 STLKLKPA 917
>NP_004490
          Length = 285

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   DATVKMKP 8
           D T+KM P
Sbjct: 98  DCTIKMDP 105
>NP_112556
          Length = 332

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   DATVKMKP 8
           D T+KM P
Sbjct: 98  DCTIKMDP 105
>NP_079321
          Length = 529

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ATVKMKP 8
           ATVK++P
Sbjct: 511 ATVKLRP 517
>NP_001257475
          Length = 1434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ATVKMKP 8
           A VKMKP
Sbjct: 461 AKVKMKP 467
>NP_061330
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ATVKMKP 8
           ATVK++P
Sbjct: 493 ATVKLRP 499
>NP_443122
          Length = 576

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ATVKMKPA 9
           ATVK +PA
Sbjct: 547 ATVKPRPA 554
>NP_619636
          Length = 760

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ATVKMKP 8
           ATVK KP
Sbjct: 104 ATVKCKP 110
>XP_005261808
          Length = 529

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ATVKMKP 8
           ATVK++P
Sbjct: 511 ATVKLRP 517
>XP_005254913
          Length = 1441

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   ATVKMKP 8
           A VKMKP
Sbjct: 461 AKVKMKP 467
>NP_733751
          Length = 4911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    TVKMKP 8
            TVK+KP
Sbjct: 4328 TVKLKP 4333
>NP_001071107
          Length = 544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   ATVKMK 7
           ATVKMK
Sbjct: 333 ATVKMK 338
>NP_060148
          Length = 260

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  ATVKMK 7
          ATVKMK
Sbjct: 49 ATVKMK 54
>XP_006716142
          Length = 4927

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    TVKMKP 8
            TVK+KP
Sbjct: 4344 TVKLKP 4349
>XP_005250088
          Length = 4928

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    TVKMKP 8
            TVK+KP
Sbjct: 4345 TVKLKP 4350
>XP_006716141
          Length = 4959

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    TVKMKP 8
            TVK+KP
Sbjct: 4376 TVKLKP 4381
>XP_005250085
          Length = 4979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    TVKMKP 8
            TVK+KP
Sbjct: 4400 TVKLKP 4405
>XP_006716140
          Length = 4982

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    TVKMKP 8
            TVK+KP
Sbjct: 4399 TVKLKP 4404
>XP_005250084
          Length = 4982

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    TVKMKP 8
            TVK+KP
Sbjct: 4399 TVKLKP 4404
>XP_005250083
          Length = 4982

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    TVKMKP 8
            TVK+KP
Sbjct: 4399 TVKLKP 4404
>XP_005250082
          Length = 4983

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    TVKMKP 8
            TVK+KP
Sbjct: 4400 TVKLKP 4405
>NP_997204
          Length = 480

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   TVKMKPA 9
           T+K+KP+
Sbjct: 382 TIKLKPS 388
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.308    0.117    0.314 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 548,072
Number of extensions: 802
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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