Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004110                                                              21     229
NP_116279                                                              17    3301
XP_006711658                                                           17    3301
XP_005273393                                                           17    3301
XP_006711657                                                           17    3301
XP_006711656                                                           17    3301
XP_005273392                                                           17    3301
XP_006711655                                                           17    3301
NP_001005514                                                           17    4312
NP_001005479                                                           17    4312
NP_000072                                                              16    7355
NP_060842                                                              16    7355
NP_001006946                                                           16    7355
XP_006711794                                                           16    7355
XP_005273086                                                           16    7355
XP_005273085                                                           16    7355
XP_005273084                                                           16    7355
XP_006710826                                                           16    7355
XP_005271086                                                           16    7355
>NP_004110
          Length = 993

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   DAQFTKMF 8
           DAQFTKMF
Sbjct: 424 DAQFTKMF 431
>NP_116279
          Length = 1524

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DAQFTKMFM 9
           DA++ K+FM
Sbjct: 765 DAEYKKLFM 773
>XP_006711658
          Length = 1235

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DAQFTKMFM 9
           DA++ K+FM
Sbjct: 476 DAEYKKLFM 484
>XP_005273393
          Length = 1330

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DAQFTKMFM 9
           DA++ K+FM
Sbjct: 571 DAEYKKLFM 579
>XP_006711657
          Length = 1330

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DAQFTKMFM 9
           DA++ K+FM
Sbjct: 571 DAEYKKLFM 579
>XP_006711656
          Length = 1524

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DAQFTKMFM 9
           DA++ K+FM
Sbjct: 765 DAEYKKLFM 773
>XP_005273392
          Length = 1524

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DAQFTKMFM 9
           DA++ K+FM
Sbjct: 765 DAEYKKLFM 773
>XP_006711655
          Length = 1524

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DAQFTKMFM 9
           DA++ K+FM
Sbjct: 765 DAEYKKLFM 773
>NP_001005514
          Length = 310

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQFTKMF 8
           A FTKMF
Sbjct: 299 ASFTKMF 305
>NP_001005479
          Length = 325

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   AQFTKMF 8
           A FTKMF
Sbjct: 315 ASFTKMF 321
>NP_000072
          Length = 3801

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    FTKMFM 9
            FTKMF+
Sbjct: 3253 FTKMFL 3258
>NP_060842
          Length = 769

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DAQFTKMF 8
           DA+F K+F
Sbjct: 567 DAEFKKIF 574
>NP_001006946
          Length = 233

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  DAQFTKMF 8
          DA+F K+F
Sbjct: 31 DAEFKKIF 38
>XP_006711794
          Length = 3022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    FTKMFM 9
            FTKMF+
Sbjct: 2474 FTKMFL 2479
>XP_005273086
          Length = 3264

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    FTKMFM 9
            FTKMF+
Sbjct: 3253 FTKMFL 3258
>XP_005273085
          Length = 3755

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    FTKMFM 9
            FTKMF+
Sbjct: 3207 FTKMFL 3212
>XP_005273084
          Length = 3801

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    FTKMFM 9
            FTKMF+
Sbjct: 3253 FTKMFL 3258
>XP_006710826
          Length = 639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DAQFTKMF 8
           DA+F K+F
Sbjct: 567 DAEFKKIF 574
>XP_005271086
          Length = 768

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DAQFTKMF 8
           DA+F K+F
Sbjct: 567 DAEFKKIF 574
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.343    0.136    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 320,255
Number of extensions: 337
Number of successful extensions: 19
Number of sequences better than 10000.0: 19
Number of HSP's gapped: 19
Number of HSP's successfully gapped: 19
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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