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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              20     509
XP_005262424                                                           20     509
NP_000261                                                              17    4312
NP_114142                                                              16    5631
XP_006711003                                                           16    5631
NP_001035829                                                           16    7355
NP_001166996                                                           16    7355
NP_004591                                                              16    7355
NP_001166995                                                           16    7355
NP_001035828                                                           16    7355
XP_006711560                                                           16    7355
XP_006711559                                                           16    7355
XP_006711558                                                           16    7355
NP_078966                                                              15    9605

>NP_722576
          Length = 3080

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DAMAMSSIP 9
            D MAMSSIP
Sbjct: 1933 DVMAMSSIP 1941



>XP_005262424
          Length = 2875

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DAMAMSSIP 9
            D MAMSSIP
Sbjct: 1728 DVMAMSSIP 1736



>NP_000261
          Length = 289

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DAMAMSSIP 9
           DA+ MS++P
Sbjct: 215 DAVGMSTVP 223



>NP_114142
          Length = 451

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AMAMSSIP 9
           A+AM+S+P
Sbjct: 170 ALAMASVP 177



>XP_006711003
          Length = 451

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   AMAMSSIP 9
           A+AM+S+P
Sbjct: 170 ALAMASVP 177



>NP_001035829
          Length = 534

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   MAMSSIP 9
           MAMS IP
Sbjct: 435 MAMSQIP 441



>NP_001166996
          Length = 518

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   MAMSSIP 9
           MAMS IP
Sbjct: 435 MAMSQIP 441



>NP_004591
          Length = 538

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   MAMSSIP 9
           MAMS IP
Sbjct: 435 MAMSQIP 441



>NP_001166995
          Length = 538

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   MAMSSIP 9
           MAMS IP
Sbjct: 435 MAMSQIP 441



>NP_001035828
          Length = 525

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   MAMSSIP 9
           MAMS IP
Sbjct: 435 MAMSQIP 441



>XP_006711560
          Length = 538

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   MAMSSIP 9
           MAMS IP
Sbjct: 435 MAMSQIP 441



>XP_006711559
          Length = 560

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   MAMSSIP 9
           MAMS IP
Sbjct: 457 MAMSQIP 463



>XP_006711558
          Length = 560

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   MAMSSIP 9
           MAMS IP
Sbjct: 457 MAMSQIP 463



>NP_078966
          Length = 14507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    DAMAMSSIP 9
            DA+A  SIP
Sbjct: 4576 DALASISIP 4584



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.115    0.300 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 276,987
Number of extensions: 263
Number of successful extensions: 14
Number of sequences better than 10000.0: 14
Number of HSP's gapped: 14
Number of HSP's successfully gapped: 14
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)